cDNA Clone MYSM1

• Synonyms: 2A-DUB, 2ADUB
• Plasmid: pMK-RQ MYSM1 Nter GFP donor
• Parent Plasmid: pMK-RQ
• Expressed: GFP-MYSM1 (following recombination)
• Genbank: ENSG00000162601
• Tag: GFP
• Species: Human

Sequence of Insert

AATCTCAGAGCTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCGGGAGGTCGGGAGTTCGAGACCAGCTGGGCAACATGGTGAAACCCTGTATCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCATGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCTGAGATACGCGCCATTGCACCCAAGCCTGGGCAACAAGAGTGAAACTCCCTCTCAAAAAAAAAAAGCTCAGGCGTGGGCCACTTCCCAAGGTCTCTACATGTCAACAAGTTAGAAAGGACCAAGCCCCAGGCCTCTGGGGGCGCCTTTGCTCTGGAAACCCCGCCTCCCGGCACGGGAGGGGCCGGACGACGCTTCAATCCCGCCCCGTGACGCGTTAGGCCCTGCCCCCGGGCCATCTTCGTCTCGCGGGATCTCTCGGGAGGACGGACGGGGTCAGGTCCCATCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGCGGCTGAAGAaGCtGAcGTcGAcATtGAgGGGGACGTGGTAGCGGCGGCGGGGGCACAGCCAGGGTGAGGCTTAGAGACCGGGTCTCTCTGGGGCGGAGAGGGGAGTGGATTGAAGGAGCGGGCCCTGGGAAAGCTGCAGGTCCGGCAAGGGAAAAGGGAACAGAAGACTGTCCCCTGGGCCAGGGTCTCTTGTTGGCCTGGGAGGCACCTCGCCCGCCGGCTTGTCCCAAGCCCAGCGGGCAGGGCGGTTACCTGGAGCTCAGGTACTCCTGGGTCGCTTTCACCTTTTGCCGGATGCTGTGAGGGCACCGATTTCGCCTACCACATTGCGTTTGATTTACATTTAGATTCCTTCTTCCGTTCCCTCCTTTTCAGGATCTCATCGACAGTAACTAGAACAAGAGCGGCTACGGACGAGACCTGAGGTCAGGGACTCTTGTTCTATGGCTTGCTAGCCGTGTGACCTTGGGCGAGTCGTTTCATCCCTTCTTCCACATGGGGATAGTTGTAAAACTTCCCGAGAAGCAGGT

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKAAEEADVDIEG
DVVAAAGAQPGSGENTASVLQKDHYLDSSWRTENGLIPWTLDNTISEENR
AVIEKMLLEEEYYLSKKSQPEKVWLDQKEDDKKYMKSLQKTAKIMVHSPT
KPASYSVKWTIEEKELFEQGLAKFGRRWTKISKLIGSRTVLQVKSYARQY
FKNKVKCGLDKETPNQKTGHNLQVKNEDKGTKAWTPSCLRGRADPNLNAV
KIEKLSDDEEVDITDEVDELSSQTPQKNSSSDLLLDFPNSKMHETNQGEF
ITSDSQEALFSKSSRGCLQNEKQDETLSSSEITLWTEKQSNGDKKSIELN
DQKFNELIKNCNKHDGRGIIVDARQLPSPEPCEIQKNLNDNEMLFHSCQM
VEESHEEEELKPPEQEIEIDRNIIQEEEKQAIPEFFEGRQAKTPERYLKI
RNYILDQWEICKPKYLNKTSVRPGLKNCGDVNCIGRIHTYLELIGAINFG
CEQAVYNRPQTVDKVRIRDRKDAVEAYQLAQRLQSMRTRRRRVRDPWGNW
CDAKDLEGQTFEHLSAEELAKRREEEKGRPVKSLKVPRPTKSSFDPFQLI
PCNFFSEEKQEPFQVKVASEALLIMDLHAHVSMAEVIGLLGGRYSEVDKV
VEVCAAEPCNSLSTGLQCEMDPVSQTQASETLAVRGFSVIGWYHSHPAFD
PNPSLRDIDTQAKYQSYFSRGGAKFIGMIVSPYNRNNPLPYSQITCLVIS
EEISPDGSYRLPYKFEVQQMLEEPQWGLVFEKTRWIIEKYRLSHSSVPMD
KIFRRDSDLTCLQKLLECMRKTLSKVTNCFMAEEFLTEIENLFLSNYKSN
QENGVTEENCTKELLM*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU48234
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to insert an in-frame GFP to the N-terminus of MYSM1 via CRISPR (exon 1). Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Sequence of cassette and amino acid sequence depict the expected fusion product following successful recombination. To be used in conjunction with 48210.