cDNA Clone KLHL3

• Synonyms: PHA2D
• Plasmid: pET15b TEV Halo KLHL3 290-end R528E
• Parent Plasmid: pET15b 6His TEV Halo 3C
• Expressed: 6xHIS-3C-TEV-Halo-3C-KLHL3 290-587 (END) R528E
• Genbank: NM_017415.2
• Tag: 6His
• Species: Human

Sequence of Insert

AGATCTATGAGGACCAAGCCCAGGACTCCAGTCAGCCTTCCCAAGGTCATGATTGTGGTTGGCGGCCAGGCACCCAAGGCAATCCGCAGTGTGGAGTGCTATGATTTCGAGGAGGACCGGTGGGATCAGATTGCTGAGCTTCCTTCCAGAAGATGCAGAGCAGGTGTGGTGTTCATGGCTGGCCACGTGTATGCCGTGGGAGGGTTTAATGGCTCACTGCGGGTGCGGACAGTGGATGTGTATGACGGCGTGAAGGACCAGTGGACGTCCATTGCCAGCATGCAGGAGCGCCGGAGCACACTGGGCGCAGCGGTGCTCAATGACTTGCTCTACGCAGTGGGAGGCTTTGATGGCAGTACTGGCCTAGCATCGGTGGAAGCCTACAGCTACAAGACCAACGAGTGGTTCTTTGTGGCCCCGATGAACACGCGGCGGAGCAGTGTGGGTGTGGGCGTTGTGGAGGGGAAGCTATATGCTGTTGGGGGTTATGATGGAGCTTCCCGCCAGTGTCTGAGCACTGTGGAGCAGTACAACCCAGCGACCAATGAATGGATATACGTGGCGGACATGAGCACCCGCCGCAGTGGCGCAGGGGTTGGAGTGCTTAGCGGACAGCTGTACGCCACAGGTGGGCATGATGGGCCTTTGGTGAGGAAGAGCGTTGAGGTTTACGATCCTGGAACAAATACCTGGAAGCAAGTGGCAGACATGAACATGTGCCGGGAGAACGCAGGGGTCTGTGCAGTAAATGGGCTCCTGTATGTGGTTGGAGGGGATGATGGATCCTGCAACTTGGCTTCGGTGGAGTACTACAATCCTGTCACTGACAAATGGACGCTGCTTCCAACGAACATGAGCACGGGGCGGAGCTATGCAGGTGTTGCCGTGATTCACAAGTCCTTGTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSENLYFQGMAEIGTGFPFDPHYVEVLGE
RMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNP
ERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVA
LVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGLEVLFQGPGSMRTKPRTPVSLPK
VMIVVGGQAPKAIRSVECYDFEEDRWDQIAELPSRRCRAGVVFMAGHVYA
VGGFNGSLRVRTVDVYDGVKDQWTSIASMQERRSTLGAAVLNDLLYAVGG
FDGSTGLASVEAYSYKTNEWFFVAPMNTRRSSVGVGVVEGKLYAVGGYDG
ASRQCLSTVEQYNPATNEWIYVADMSTRRSGAGVGVLSGQLYATGGHDGP
LVRKSVEVYDPGTNTWKQVADMNMCRENAGVCAVNGLLYVVGGDDGSCNL
ASVEYYNPVTDKWTLLPTNMSTGRSYAGVAVIHKSL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU48245
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 290-587 (END), R528E. Use TEV to cleave off 6xHIS, precission may be used downstream to remove the HALO tag if necessary.