cDNA Clone LRRC8A

• Synonyms: AGM5, LRRC8
• Plasmid: pCMV5D GFP LRRC8A R389A
• Parent Plasmid: pCMV5D GFP
• Expressed: GFP-LRRC8A R389A
• Genbank: NM_001127244.1
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCGCCATGATTCCGGTGACAGAGCTCCGCTACTTTGCGGACACGCAGCCAGCATACCGGATCCTGAAGCCGTGGTGGGATGTGTTCACAGACTACATCTCTATCGTCATGCTGATGATTGCCGTCTTCGGGGGGACGCTGCAGGTCACCCAAGACAAGATGATCTGCCTGCCTTGTAAGTGGGTCACCAAGGACTCCTGCAATGATTCGTTCCGGGGCTGGGCAGCCCCTGGCCCGGAGCCCACCTACCCCAACTCCACCATTCTGCCGACCCCTGACACGGGCCCCACAGGCATCAAGTATGACCTGGACCGGCACCAGTACAACTACGTGGACGCTGTGTGCTATGAGAACCGACTGCACTGGTTTGCCAAGTACTTCCCCTACCTGGTGCTTCTGCACACGCTCATCTTCCTGGCCTGCAGCAACTTCTGGTTCAAATTCCCGCGCACCAGCTCGAAGCTGGAGCACTTTGTGTCTATCCTGCTGAAGTGCTTCGACTCGCCCTGGACCACGAGGGCCCTGTCGGAGACAGTGGTGGAGGAGAGCGACCCCAAGCCGGCCTTCAGCAAGATGAATGGGTCCATGGACAAAAAGTCATCGACCGTCAGTGAGGACGTGGAGGCCACCGTGCCCATGCTGCAGCGGACCAAGTCACGGATCGAGCAGGGTATCGTGGACCGCTCAGAGACGGGCGTGCTGGACAAGAAGGAGGGGGAGCAAGCCAAGGCGCTGTTTGAGAAGGTGAAGAAGTTCCGGACCCATGTGGAGGAGGGGGACATTGTGTACCGCCTCTACATGCGGCAGACCATCATCAAGGTGATCAAGTTCATCCTCATCATCTGCTACACCGTCTACTACGTGCACAACATCAAGTTCGACGTGGACTGCACCGTGGACATTGAGAGCCTGACGGGCTACCGCACCTACCGCTGTGCCCACCCCCTGGCCACACTCTTCAAGATCCTGGCGTCCTTCTACATCAGCCTAGTCATCTTCTACGGCCTCATCTGCATGTATACACTGTGGTGGATGCTACGGCGCTCCCTCAAGAAGTACTCGTTTGAGTCGATCCGTGAGGAGAGCAGCTACAGCGACATCCCCGACGTCAAGAACGACTTCGCCTTCATGCTGCACCTCATTGACCAATACGACCCGCTCTACTCCAAGGCATTCGCCGTCTTCCTGTCGGAGGTGAGTGAGAACAAGCTGCGGCAGCTGAACCTCAACAACGAGTGGACGCTGGACAAGCTCCGGCAGCGGCTCACCAAGAACGCGCAGGACAAGCTGGAGCTGCACCTGTTCATGCTCAGTGGCATCCCTGACACTGTGTTTGACCTGGTGGAGCTGGAGGTCCTCAAGCTGGAGCTGATCCCCGACGTGACCATCCCGCCCAGCATTGCCCAGCTCACGGGCCTCAAGGAGCTGTGGCTCTACCACACAGCGGCCAAGATTGAAGCGCCCGCGCTGGCCTTCCTGCGCGAGAACCTGCGGGCGCTGCACATCAAGTTCACCGACATCAAGGAGATCCCGCTGTGGATCTATAGCCTGAAGACACTGGAGGAGCTGCACCTGACGGGCAACCTGAGCGCGGAGAACAACCGCTACATCGTCATCGACGGGCTGCGGGAGCTCAAACGCCTCAAGGTGCTGCGGCTCAAGAGCAACCTAAGCAAGCTGCCACAGGTGGTCACAGATGTGGGCGTGCACCTGCAGAAGCTGTCCATCAACAATGAGGGCACCAAGCTCATCGTCCTCAACAGCCTCAAGAAGATGGCGAACCTGACTGAGCTGGAGCTGATCCGCTGTGACCTGGAGCGCATCCCCCACTCCATCTTCAGCCTCCACAACCTGCAGGAGATTGACCTCAAGGACAACAACCTCAAGACCATCGAGGAGATCATCAGCTTCCAGCACCTGCACCGCCTCACCTGCCTTAAGCTGTGGTACAACCACATCGCCTACATCCCCATCCAGATCGGCAACCTCACCAACCTGGAGCGCCTCTACCTGAACCGCAACAAGATCGAGAAGATCCCCACCCAGCTCTTCTACTGCCGCAAGCTGCGCTACCTGGACCTCAGCCACAACAACCTGACCTTCCTCCCTGCCGACATCGGCCTCCTGCAGAACCTCCAGAACCTAGCCATCACGGCCAACCGGATCGAGACGCTCCCTCCGGAGCTCTTCCAGTGCCGGAAGCTGCGGGCCCTGCACCTGGGCAACAACGTGCTGCAGTCACTGCCCTCCAGGGTGGGCGAGCTGACCAACCTGACGCAGATCGAGCTGCGGGGCAACCGGCTGGAGTGCCTGCCTGTGGAGCTGGGCGAGTGCCCACTGCTCAAGCGCAGCGGCTTGGTGGTGGAGGAGGACCTGTTCAACACACTGCCACCCGAGGTGAAGGAGCGGCTGTGGAGGGCTGACAAGGAGCAGGCCTGAGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSFEEFAMIP
VTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMI
CLPCKWVTKDSCNDSFRGWAAPGPEPTYPNSTILPTPDTGPTGIKYDLDR
HQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKL
EHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSE
DVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTH
VEEGDIVYRLYMRQTIIKVIKFILIICYTVYYVHNIKFDVDCTVDIESLT
GYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSF
ESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKAFAVFLSEVSENKLR
QLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKL
ELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFT
DIKEIPLWIYSLKTLEELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKS
NLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMANLTELELIRCDL
ERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAY
IPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPAD
IGLLQNLQNLAITANRIETLPPELFQCRKLRALHLGNNVLQSLPSRVGEL
TNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFNTLPPEVKERLW
RADKEQA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48266
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: R389A