cDNA Clone Notch1

• Plasmid: pCMV5D GFP Notch1 V1754-K2555(end)-HA
• Parent Plasmid: pCMV5D GFP
• Expressed: GFP Notch1 V1754-K2555(end)-HA
• Genbank: P46531 (codon optimised)
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCATGGTGCTGCTGAGCCGGAAGAGAAGAAGGCAGCACGGCCAGCTGTGGTTCCCCGAGGGCTTTAAGGTGTCCGAGGCCAGCAAGAAGAAGCGGAGAGAGCCCCTGGGCGAGGATAGCGTGGGACTGAAGCCCCTGAAGAACGCCTCTGACGGCGCCCTGATGGACGACAACCAGAACGAATGGGGCGACGAGGACCTGGAAACAAAGAAGTTCAGATTCGAGGAACCCGTGGTGCTGCCCGACCTGGACGATCAGACCGACCACAGACAGTGGACCCAGCAGCATCTGGACGCCGCCGACCTGAGAATGTCTGCCATGGCCCCTACACCCCCTCAGGGCGAAGTGGACGCCGACTGCATGGATGTGAACGTGCGGGGACCCGACGGCTTCACCCCTCTGATGATCGCCAGCTGTTCTGGCGGCGGACTGGAAACCGGCAACAGCGAGGAAGAAGAGGACGCCCCTGCCGTGATCAGCGACTTCATATATCAGGGGGCCAGCCTGCACAACCAGACCGACAGAACAGGCGAGACAGCCCTGCATCTGGCCGCTCGGTACAGCAGATCTGATGCCGCCAAGCGGCTGCTGGAAGCCAGCGCCGATGCCAACATCCAGGACAACATGGGCAGAACCCCTCTGCATGCCGCCGTGTCTGCTGATGCTCAGGGCGTGTTCCAGATCCTGATCCGGAACAGAGCCACCGATCTGGATGCCCGGATGCACGATGGCACCACCCCACTGATTCTGGCCGCCAGACTGGCCGTGGAAGGCATGCTGGAAGATCTGATCAACAGCCACGCCGACGTGAACGCCGTGGACGATCTGGGAAAGAGCGCCCTGCATTGGGCAGCCGCCGTGAACAATGTGGACGCTGCTGTGGTGCTGCTGAAGAACGGCGCCAACAAGGACATGCAGAACAACAGAGAGGAAACCCCCCTGTTCCTGGCCGCTAGAGAGGGCAGCTACGAAACAGCCAAAGTGCTGCTGGATCACTTCGCCAACCGGGACATCACCGATCACATGGACAGACTGCCCCGGGATATCGCCCAGGAACGGATGCACCACGACATCGTGCGCCTGCTGGACGAGTACAACCTCGTGCGCTCTCCCCAGCTGCATGGCGCTCCTCTGGGAGGCACACCTACACTGAGCCCTCCACTGTGCAGCCCCAATGGCTACCTGGGCTCTCTGAAGCCTGGCGTGCAGGGCAAGAAAGTGCGGAAGCCTAGCAGCAAGGGCCTGGCCTGTGGCAGCAAAGAGGCCAAGGACCTGAAGGCCAGACGGAAGAAGTCCCAGGACGGCAAGGGATGTCTGCTGGACAGCAGCGGCATGCTGAGCCCCGTGGATAGCCTGGAAAGCCCTCACGGCTACCTGAGCGACGTGGCCTCTCCACCTCTGCTGCCTAGCCCTTTCCAGCAGAGCCCTAGCGTGCCCCTGAACCATCTGCCCGGCATGCCTGATACCCACCTGGGAATCGGCCACCTGAACGTGGCCGCCAAACCTGAAATGGCCGCACTGGGAGGCGGAGGCAGACTGGCTTTTGAGACAGGCCCCCCTCGGCTGTCCCATCTGCCAGTGGCAAGCGGCACAAGCACAGTGCTGGGCTCTAGCTCTGGCGGAGCCCTGAATTTCACAGTGGGCGGCAGCACCTCCCTGAACGGACAGTGTGAATGGCTGAGCAGACTGCAGAGCGGCATGGTGCCCAACCAGTACAACCCCCTGAGAGGCTCTGTGGCCCCTGGCCCACTGTCTACACAGGCCCCATCTCTGCAGCACGGAATGGTGGGACCTCTGCACAGCAGCCTGGCTGCCTCTGCCCTGAGCCAGATGATGAGCTACCAGGGCCTGCCCAGCACAAGACTGGCCACACAGCCACACCTGGTGCAGACACAGCAGGTGCAGCCTCAGAACCTGCAGATGCAGCAGCAGAATCTGCAGCCCGCCAACATTCAACAGCAGCAGTCCCTGCAGCCCCCTCCACCTCCTCCACAGCCTCATCTGGGAGTGTCTAGCGCCGCCTCTGGCCACCTGGGCAGAAGCTTTCTGAGCGGCGAACCCTCCCAGGCCGATGTGCAGCCACTGGGACCATCTTCCCTGGCTGTGCACACCATCCTGCCTCAGGAAAGCCCAGCCCTGCCTACCAGCCTGCCTAGCTCTCTGGTGCCTCCTGTGACAGCCGCCCAGTTCCTGACACCTCCTAGCCAGCACAGCTACAGCTCCCCCGTGGACAATACCCCCAGCCACCAGCTGCAGGTGCCCGAGCACCCATTTCTGACCCCTAGCCCCGAGAGCCCCGATCAGTGGTCTAGCAGCAGCCCCCACAGCAACGTGTCCGATTGGAGCGAAGGCGTGTCCAGCCCCCCAACCAGCATGCAGTCTCAGATCGCCAGAATCCCCGAGGCCTTCAAGGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSAMVLLSRK
RRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDN
QNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMA
PTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAV
ISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDN
MGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEG
MLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNN
REETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDI
VRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVR
KPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGY
LSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMAALG
GGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGSTSLNGQCE
WLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASAL
SQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANIQQQQSLQ
PPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLAVHTILPQ
ESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPF
LTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFKAAAY
PYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49637
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee