cDNA Clone MCM7

• Plasmid: pRS305-Mcm7 K29A, K59A, K62A, K69A and K92A
• Parent Plasmid: pRS305
• Expressed: pMM33
• Species: Saccharomyces cerevisiae

Amino Acid Sequence

MSAALPSIQLPVDYNNLFNEITDFLVTFAQDTLSSDATRNENEDENLDAE
NIEQHLLEAGPAYMAMLQAVANRELNSVIIDLDDILQYQNEAFLQGTQAD
DLVSAIQQNANHFTELFCRAIDNNMPLPTKEIDYKDDVLDVILNQRRLRN
ERMLSDRTNEIRSENLMDTTMDPPSSMNDALREVVEDETELFPPNLTRRY
FLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLITVRGIITRVSD
VKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVD
VTGIFLPAPYTGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEER
VMELITSGDVYNRLAKSIAPEIYGNLDVKKALLLLLVGGVDKRVGDGMKI
RGDINVCLMGDPGVAKSQLLKAICKISPRGVYTTGKGSSGVGLTAAVMKD
PVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEVMEQQTISISK
AGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRP
VMSEAVNDYVVQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALA
KLRLADMVDIDDVEEALRLVRVSKESLYQETNKSKEDESPTTKIFTIIKK
MLQETGKNTLSYENIVKTVRLRGFTMLQLSNCIQEYSYLNVWHLINEGNT
LKFVDDGTMDTDQEDSLVSTPKLAPQTTASANASAQDSDIDLQDA*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU24460
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: relative to the wild type gene in ‘W303’ strain