cDNA Clone Fancd2 (mouse)

• Synonyms: 2410150O07Rik; AU015151; BB137857; FA-D2; FA4; FACD; FAD; FANCD
• Plasmid: pBabeD neo mCherry mouse fancd2 K559R
• Parent Plasmid: pBabeD neo mCherry
• Expressed: mCherry mouse fancd2 K559R
• Genbank: NM_001033244.3 GI:282721082 with silent change t4203c to kill internal BamHI
• Tag: mCherry
• Species: Mouse

Sequence of Insert

GGATCCATGATTTCCAAAAGACGTCGGCTAGATTCTGAGGATAAAGAAAACCTGACAGAAGATGCCTCCAAAACCATGCCCCTTTCCAAGCTGGCAAAGAAGTCTCACAATTCTCATGAAGTTGAAGAAAATGGCAGTGTCTTTGTAAAGCTTCTTAAGGCTTCAGGACTCACTCTTAAAACTGGAGAGAACCAAAATCAGCTAGGTGTGGATCAGGTAATCTTCCAAAGGAAGCTCTTTCAGGCCTTGAGGAAGCATCCTGCTTATCCCAAAGTAATAGAAGAGTTTGTTAATGGCCTGGAGTCCTACACTGAGGACAGTGAGAGTCTCAGGAACTGCCTGCTGTCTTGTGAGCGCCTGCAGGATGAGGAAGCCAGCATGGGCACATTTTACTCCAAGAGTCTGATCAAGCTACTTCTGGGGATTGACATTTTACAGCCTGCCATTATCAAAATGTTATTTGAAAAAGTGCCTCAGTTTCTTTTTGAAAGTGAGAACAGAGATGGAATCAACATGGCCAGACTCATTATCAATCAACTAAAATGGCTGGATAGAATTGTGGATGGCAAGGACCTCACGGCCCAGATGATGCAGTTGATCAGTGTTGCTCCCGTGAACTTACAGCATGACTTCATCACGAGCCTTCCTGAAATCCTAGGGGATTCCCAGCATGCTAATGTGGGGAAAGAGCTTGGCGAGCTGCTGGTGCAGAATACTTCCCTGACTGTTCCAATTTTGGATGTCTTTTCCAGTCTCCGACTTGACCCCAACTTCCTGTCCAAGATCCGCCAGTTGGTGATGGGCAAGCTGTCATCTGTCCGTCTAGAGGATTTCCCTGTGATTGTAAAGTTCCTTCTTCATTCTGTAACAGACACCACTTCCCTTGAGGTCATTGCCGAGCTTCGGGAGAACTTGAACGTCCAGCAGTTTATTTTGCCGTCACGAATTCAGGCTTCCCAAAGCAAATTGAAAAGTAAAGGACTAGCAAGCTCTTCAGGAAATCAAGAGAACAGTGATAAAGACTGTATTGTTCTTGTCTTTGATGTAATAAAGTCAGCCATTAGATATGAGAAAACCATTTCAGAGGCCTGGTTTAAGGCAATTGAACGCATTGAGTCCGCGGCTGAACATAAGGCTTTGGACGTGGTCATGCTGCTCATCATCTACAGCACCAGCACGCAGACCAAGAAGGGCGTGGAGAAGCTGCTGAGAAACAAGATTCAGTCAGACTGCATTCAAGAACAGCTGCTTGACAGTGCGTTCTCTACACATTACCTGGTTCTTAAGGATATTTGCCCATCTATTCTTTTGCTGGCTCAGACTTTGTTTCACTCTCAAGACCAGAGGATCATTTTGTTTGGCAGTCTTCTGTACAAATATGCTTTTAAGTTTTTTGATACTTACTGCCAGCAGGAAGTGGTTGGTGCCCTAGTCACCCATGTCTGCAGTGGGACTGAGGCTGAAGTCGACACTGCACTGGATGTCCTCCTGGAGCTGATTGTGCTAAACGCCTCTGCTATGAGGCTCAATGCTGCTTTTGTTAAGGGCATCTTAGATTATTTGGAAAATATGTCCCCTCAGCAAATACGAAAAATCTTCTGTATTCTCAGCACTCTTGCATTTAGCCAACAGCCCGGAACCAGCAACCATATCCAGGACGACATGCACCTGGTGATCCGGAGGCAGCTCTCTAGCACTGTGTTCAAGTACAAGCTCATTGGGATCATTGGTGCAGTCACCATGGCCGGCATCATGGCGGAAGACAGAAGTGTACCATCTAACTCATCCCAGAGGAGCGCCAATGTGAGCAGTGAGCAGCGCACACAGGTGACTTCTTTGCTACAACTAGTTCATTCTTGCACTGAGCACTCTCCTTGGGCCTCTTCTCTGTATTATGATGAATTTGCCAACCTGATCCAAGAAAGGAAGTTGGCTCCAAAAACCTTGGAGTGGGTTGGGCAGACCATCTTCAATGATTTCCAAGATGCCTTTGTGGTAGACTTCTGTGCTGCTCCAGAGGGTGACTTTCCATTTCCTGTGAAAGCGCTCTATGGACTGGAAGAGTACAGCACTCAAGACGGCATTGTCATCAACCTCCTGCCGCTGTTCTATCAGGAATGTGCAAAAGATGCCAGTCGAGCGACATCACAAGAATCGAGCCAGAGATCAATGTCTTCTTTGTGCCTGGCTTCCCATTTCCGGCTGCTGAGACTTTGCGTGGCAAGACAACATGATGGAAACTTGGATGAGATCGATGGTCTCTTAGATTGTCCCCTGTTCCTCCCTGACCTGGAACCTGGAGAGAAACTGGAGTCCATGTCTGCTAAAGACCGTTCGCTTATGTGTTCGCTCACATTCCTAACTTTCAACTGGTTCCGAGAGGTTGTGAATGCCTTCTGCCAACAAACATCTCCTGAGATGAAGGGCAAGGTTCTTAGTCGGCTAAAGGACCTTGTAGAACTTCAGGGAATCCTAGAGAAGTACTTGGCAGTCATCCCAGACTATGTTCCGCCTTTCGCAAGCGTTGACTTGGACACTTTAGATATGATGCCTAGGAGCAGTTCTGCTGTTGCAGCAAAAAACAGAAACAAGGGAAAGACGGGGGGAAAGAAACAAAAAGCTGATAGCAACAAAGCATCCTGTTCGGACACACTTCTAACAGAAGACACTTCAGAGTGTGACATGGCGCCATCTGGGAGAAGCCACGTAGACAAGGAGTCCACAGGGAAGGAAGGAAAGACGTTTGTGTCACTGCAGAATTACCGCGCTTTTTTCCGAGAGCTGGACATTGAGGTCTTCTCTATTCTACATTCTGGACTTGTGACCAAGTTCATCTTAGACACTGAAATGCACACTGAAGCTACAGAGGTCGTACAGCTGGGGCCTGCTGAGCTGCTCTTCTTGCTGGAAGATCTTTCCCAGAAGCTAGAGAATATGCTGACTGCTCCTTTTGCCAAGAGAATCTGCTGCTTTAAGAATAAAGGAAGGCAGAATATTGGCTTCTCACATCTTCATCAGAGATCTGTCCAGGACATTGTGCACTGTGTGGTTCAGCTGCTAACCCCGATGTGTAACCATCTGGAGAACATTCACAACTTCTTTCAGTGCTTAGGTGCTGAGCATCTCAGTGCAGATGACAAGGCGAGAGCGACAGCTCAGGAGCAGCACACCATGGCCTGCTGCTACCAGAAGCTGCTGCAGGTCTTGCACGCGCTCTTTGCGTGGAAGGGATTTACTCACCAATCAAAGCACCGCCTCCTGCACTCAGCCCTTGAGGTCCTCTCGAACCGACTAAAGCAGATGGAACAGGACCAGCCCTTGGAGGAACTGGTCAGCCAGAGCTTCAGTTACTTGCAGAACTTCCACCATAGTGTTCCCAGTTTCCAGTGTGGTCTCTACCTTCTCAGACTTCTGATGGCCCTTCTGGAGAAGTCTGCAGTACCTAACCAGAAGAAAGAAAAACTTGCCTCTCTGGCCAAACAGCTGCTTTGCCGAGCATGGCCTCATGGGGAAAAAGAGAAGAACCCCACTTTTAATGACCACCTGCATGATGTGCTTTACATCTACTTGGAGCACACAGACAATGTTCTGAAGGCCATAGAGGAGATCACTGGTGTTGGTGTCCCAGAACTGGTCAGTGCTCCGAAAGACGCCGCCTCCTCTACATTCCCTACGTTGACCAGGCACACCTTTGTCATATTCTTCCGTGTGATGATGGCTGAACTCGAGAAGACGGTGAAGGGTCTTCAGGCTGGCACAGCAGCAGATTCGCAGCAGGTTCACGAAGAGAAGCTCCTCTATTGGAACATGGCTGTCCGAGATTTCAGCATCCTTCTCAATCTGATGAAAGTATTTGACAGTTATCCTGTTCTGCATGTGTGTTTAAAGTATGGCCGTCGCTTTGTGGAGGCATTTCTGAAGCAATGTATGCCACTCCTCGACTTCAGCTTTAGAAAGCATCGGGAAGATGTTCTGAGCTTGCTGCAAACCCTTCAGTTGAACACGAGGCTACTTCATCACCTTTGTGGACACTCCAAGATTCGCCAGGACACAAGACTCACCAAGCATGTGCCTTTACTCAAAAAGTCACTGGAACTGTTAGTTTGCAGAGTCAAAGCCATGCTTGTCCTCAACAACTGTAGAGAGGCTTTCTGGTTGGGTACTCTCAAAAACCGAGACTTACAGGGTGAAGAAATTATTTCCCAGGACCCCTCTTCCTCAGAGAGCAATGCAGAGGACAGTGAGGATGGCGTGACATCTCACGTCTCCAGGAACAGAGCAACAGAGGATGGGGAAGATGAAGCAAGTGATGAACAGAAGGACCAGGACAGTGATGAAAGTGACGACAGCTCCAGTTAGCGGCCGC

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKGGGSMISKRRRLDS
EDKENLTEDASKTMPLSKLAKKSHNSHEVEENGSVFVKLLKASGLTLKTG
ENQNQLGVDQVIFQRKLFQALRKHPAYPKVIEEFVNGLESYTEDSESLRN
CLLSCERLQDEEASMGTFYSKSLIKLLLGIDILQPAIIKMLFEKVPQFLF
ESENRDGINMARLIINQLKWLDRIVDGKDLTAQMMQLISVAPVNLQHDFI
TSLPEILGDSQHANVGKELGELLVQNTSLTVPILDVFSSLRLDPNFLSKI
RQLVMGKLSSVRLEDFPVIVKFLLHSVTDTTSLEVIAELRENLNVQQFIL
PSRIQASQSKLKSKGLASSSGNQENSDKDCIVLVFDVIKSAIRYEKTISE
AWFKAIERIESAAEHKALDVVMLLIIYSTSTQTKKGVEKLLRNKIQSDCI
QEQLLDSAFSTHYLVLKDICPSILLLAQTLFHSQDQRIILFGSLLYKYAF
KFFDTYCQQEVVGALVTHVCSGTEAEVDTALDVLLELIVLNASAMRLNAA
FVKGILDYLENMSPQQIRKIFCILSTLAFSQQPGTSNHIQDDMHLVIRRQ
LSSTVFKYKLIGIIGAVTMAGIMAEDRSVPSNSSQRSANVSSEQRTQVTS
LLQLVHSCTEHSPWASSLYYDEFANLIQERKLAPKTLEWVGQTIFNDFQD
AFVVDFCAAPEGDFPFPVKALYGLEEYSTQDGIVINLLPLFYQECAKDAS
RATSQESSQRSMSSLCLASHFRLLRLCVARQHDGNLDEIDGLLDCPLFLP
DLEPGEKLESMSAKDRSLMCSLTFLTFNWFREVVNAFCQQTSPEMKGKVL
SRLKDLVELQGILEKYLAVIPDYVPPFASVDLDTLDMMPRSSSAVAAKNR
NKGKTGGKKQKADSNKASCSDTLLTEDTSECDMAPSGRSHVDKESTGKEG
KTFVSLQNYRAFFRELDIEVFSILHSGLVTKFILDTEMHTEATEVVQLGP
AELLFLLEDLSQKLENMLTAPFAKRICCFKNKGRQNIGFSHLHQRSVQDI
VHCVVQLLTPMCNHLENIHNFFQCLGAEHLSADDKARATAQEQHTMACCY
QKLLQVLHALFAWKGFTHQSKHRLLHSALEVLSNRLKQMEQDQPLEELVS
QSFSYLQNFHHSVPSFQCGLYLLRLLMALLEKSAVPNQKKEKLASLAKQL
LCRAWPHGEKEKNPTFNDHLHDVLYIYLEHTDNVLKAIEEITGVGVPELV
SAPKDAASSTFPTLTRHTFVIFFRVMMAELEKTVKGLQAGTAADSQQVHE
EKLLYWNMAVRDFSILLNLMKVFDSYPVLHVCLKYGRRFVEAFLKQCMPL
LDFSFRKHREDVLSLLQTLQLNTRLLHHLCGHSKIRQDTRLTKHVPLLKK
SLELLVCRVKAMLVLNNCREAFWLGTLKNRDLQGEEIISQDPSSSESNAE
DSEDGVTSHVSRNRATEDGEDEASDEQKDQDSDESDDSSS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49535
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee