cDNA Clone Notch1

• Synonyms: TAN1, hN1
• Plasmid: pMK-RQ NOTCH1 1754-end no stop Bam Not
• Parent Plasmid: pMK-RQ
• Genbank: P46531 (codon optimised)
• Species: Human

Sequence of Insert

GGATCCGCCATGGTGCTGCTGAGCCGGAAGAGAAGAAGGCAGCACGGCCAGCTGTGGTTCCCCGAGGGCTTTAAGGTGTCCGAGGCCAGCAAGAAGAAGCGGAGAGAGCCCCTGGGCGAGGATAGCGTGGGACTGAAGCCCCTGAAGAACGCCTCTGACGGCGCCCTGATGGACGACAACCAGAACGAATGGGGCGACGAGGACCTGGAAACAAAGAAGTTCAGATTCGAGGAACCCGTGGTGCTGCCCGACCTGGACGATCAGACCGACCACAGACAGTGGACCCAGCAGCATCTGGACGCCGCCGACCTGAGAATGTCTGCCATGGCCCCTACACCCCCTCAGGGCGAAGTGGACGCCGACTGCATGGATGTGAACGTGCGGGGACCCGACGGCTTCACCCCTCTGATGATCGCCAGCTGTTCTGGCGGCGGACTGGAAACCGGCAACAGCGAGGAAGAAGAGGACGCCCCTGCCGTGATCAGCGACTTCATATATCAGGGGGCCAGCCTGCACAACCAGACCGACAGAACAGGCGAGACAGCCCTGCATCTGGCCGCTCGGTACAGCAGATCTGATGCCGCCAAGCGGCTGCTGGAAGCCAGCGCCGATGCCAACATCCAGGACAACATGGGCAGAACCCCTCTGCATGCCGCCGTGTCTGCTGATGCTCAGGGCGTGTTCCAGATCCTGATCCGGAACAGAGCCACCGATCTGGATGCCCGGATGCACGATGGCACCACCCCACTGATTCTGGCCGCCAGACTGGCCGTGGAAGGCATGCTGGAAGATCTGATCAACAGCCACGCCGACGTGAACGCCGTGGACGATCTGGGAAAGAGCGCCCTGCATTGGGCAGCCGCCGTGAACAATGTGGACGCTGCTGTGGTGCTGCTGAAGAACGGCGCCAACAAGGACATGCAGAACAACAGAGAGGAAACCCCCCTGTTCCTGGCCGCTAGAGAGGGCAGCTACGAAACAGCCAAAGTGCTGCTGGATCACTTCGCCAACCGGGACATCACCGATCACATGGACAGACTGCCCCGGGATATCGCCCAGGAACGGATGCACCACGACATCGTGCGCCTGCTGGACGAGTACAACCTCGTGCGCTCTCCCCAGCTGCATGGCGCTCCTCTGGGAGGCACACCTACACTGAGCCCTCCACTGTGCAGCCCCAATGGCTACCTGGGCTCTCTGAAGCCTGGCGTGCAGGGCAAGAAAGTGCGGAAGCCTAGCAGCAAGGGCCTGGCCTGTGGCAGCAAAGAGGCCAAGGACCTGAAGGCCAGACGGAAGAAGTCCCAGGACGGCAAGGGATGTCTGCTGGACAGCAGCGGCATGCTGAGCCCCGTGGATAGCCTGGAAAGCCCTCACGGCTACCTGAGCGACGTGGCCTCTCCACCTCTGCTGCCTAGCCCTTTCCAGCAGAGCCCTAGCGTGCCCCTGAACCATCTGCCCGGCATGCCTGATACCCACCTGGGAATCGGCCACCTGAACGTGGCCGCCAAACCTGAAATGGCCGCACTGGGAGGCGGAGGCAGACTGGCTTTTGAGACAGGCCCCCCTCGGCTGTCCCATCTGCCAGTGGCAAGCGGCACAAGCACAGTGCTGGGCTCTAGCTCTGGCGGAGCCCTGAATTTCACAGTGGGCGGCAGCACCTCCCTGAACGGACAGTGTGAATGGCTGAGCAGACTGCAGAGCGGCATGGTGCCCAACCAGTACAACCCCCTGAGAGGCTCTGTGGCCCCTGGCCCACTGTCTACACAGGCCCCATCTCTGCAGCACGGAATGGTGGGACCTCTGCACAGCAGCCTGGCTGCCTCTGCCCTGAGCCAGATGATGAGCTACCAGGGCCTGCCCAGCACAAGACTGGCCACACAGCCACACCTGGTGCAGACACAGCAGGTGCAGCCTCAGAACCTGCAGATGCAGCAGCAGAATCTGCAGCCCGCCAACATTCAACAGCAGCAGTCCCTGCAGCCCCCTCCACCTCCTCCACAGCCTCATCTGGGAGTGTCTAGCGCCGCCTCTGGCCACCTGGGCAGAAGCTTTCTGAGCGGCGAACCCTCCCAGGCCGATGTGCAGCCACTGGGACCATCTTCCCTGGCTGTGCACACCATCCTGCCTCAGGAAAGCCCAGCCCTGCCTACCAGCCTGCCTAGCTCTCTGGTGCCTCCTGTGACAGCCGCCCAGTTCCTGACACCTCCTAGCCAGCACAGCTACAGCTCCCCCGTGGACAATACCCCCAGCCACCAGCTGCAGGTGCCCGAGCACCCATTTCTGACCCCTAGCCCCGAGAGCCCCGATCAGTGGTCTAGCAGCAGCCCCCACAGCAACGTGTCCGATTGGAGCGAAGGCGTGTCCAGCCCCCCAACCAGCATGCAGTCTCAGATCGCCAGAATCCCCGAGGCCTTCAAGGCGGCCGC

Amino Acid Sequence

MVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASD
GALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAAD
LRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEE
EEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASA
DANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILA
ARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGA
NKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQ
ERMHHDIVRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPG
VQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDS
LESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAK
PEMAALGGGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGST
SLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHS
SLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANI
QQQQSLQPPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLA
VHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQL
QVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPE
AFK

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU48379
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 1754-end. Holding vector for synthesised fragment.; Codon optimised sequence