cDNA Clone DCUN1D3

• Synonyms: 44M2.4
• Plasmid: pU6 DCUN1D3 gRNA B
• Parent Plasmid: pU6 gRNA BamHI
• Expressed: DCUN1D3 gRNA B
• Genbank: ENSG00000188215
• Species: Human

Sequence of Insert

gAGAATCCCTCATCGACCCT(GGG)

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48395
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Expresses DCUN1D3 guide RNA B, 19mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets (extra g added to aid expression). Targets coding region in exon 2, PAM site is situated on the sense strand. To be used in conjunction with DCUN1D3 guide RNA A, a ssDNA KO donor and Cas9 nickase.