cDNA Clone Mms22

• Plasmid: pGADT7 + linker Yeast MMS22 V542-V712
• Parent Plasmid: pGADT7
• Expressed: GAL4 AD-HA-Linker+Yeast Mms22 V542-V712
• Genbank: DAA09629.1
• Tag: HA
• Species: Yeast

Sequence of Insert

GTTCCCACGAACCGATACGCTTTCACAAAGCCAAATGTACGTAATAGGGACAGCATTAATCATGACATGGAATTTGAGGAAGAAGACTCCAATCAAGAGCTTGGACCAATAATGGTCGTACTTGACTCAATTTTACTAAAAAAACCTTTTGAGCCACCTAATTTCTTCAAGATCCAGTTGTCAGACAAGAGTTTCTTGCTATCAAAATTGAATCCTGCAGATATAGCTACATCATTACAGAAAATATTCAGAGTAATCATTGATAAAGGTATCACTGATACTGAACTAGTTCACTTTAACGAAAGCCTTATTGCTTTTTTGGTGCACCTTGACATGCCTGAACTATTCGACTTAATAGGTGAATTTCATCGTGAATTTAGATCGAAGGTCAATAGCTTGAGGAAAAAAGCTAAACCAATCCATTTTTTTCAAATTGCGGCATGCCAATTAATGTTTCTAGAAATATCAAGATATAACAAAATTTCAGCCGCTGCAAAATTCGACATGGATGTCTGA

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAAMEYPYDVPDYAHMAMEASEFHPGGAGAGAGAGAVPTNRYAFTKPNVRNRDSINHDMEFEEEDSNQELGPIMVVLDSILLKKPFEPPNFFKIQLSDKSFLLSKLNPADIATSLQKIFRVIIDKGITDTELVHFNESLIAFLVHLDMPELFDLIGEFHREFRSKVNSLRKKAKPIHFFQIAACQLMFLEISRYNKISAAAKFDMDV*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU25672
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Stop mutated in at aa713 to create fragment