cDNA Clone Npl4 (yeast)

• Synonyms: HRD4
• Plasmid: pET15 6His 3C R1 yeast Npl4 M1-S222
• Parent Plasmid: pET15b 6His 3C R1
• Expressed: 6His 3C yeast Npl4 M1-S222
• Genbank: NM_001178518.1 with silent change a282c
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCATGCTTATCAGATTTAGATCAAAAAACGGTACACACAGGGTTTCTTGTCAAGAAAACGACCTTTTCGGAACGGTCATTGAAAAGTTGGTGGGTAATCTGGACCCCAATGCCGATGTCGACACATTTACTGTTTGTGAGAAGCCCGGTCAAGGTATTCATGCTGTTTCTGAACTAGCTGATCGAACAGTGATGGATCTAGGACTAAAGCACGGTGACATGCTGATCCTTAACTATTCGGACAAGCCCGCTAATGAGAAAGATGGTGTCAATGTTGAAATCGGCTCCGTAGGTATTGACAGCAAGGGAATTCGTCAACACAGGTACGGTCCACTAAGGATCAAAGAACTCGCTGTAGATGAGGAACTAGAGAAAGAGGACGGATTAATTCCTCGTCAAAAATCAAAACTATGCAAGCACGGTGATAGAGGTATGTGTGAATACTGCTCGCCTTTACCTCCTTGGGACAAAGAATATCATGAGAAGAATAAGATCAAACATATATCGTTTCATTCATATCTTAAAAAGCTAAATGAAAATGCTAATAAGAAGGAGAACGGCAGCTCTTATATTTCCCCTCTTTCAGAACCTGATTTTAGAATCAATAAGCGTTGCCATAATGGCCATGAACCATGGCCTCGGGGGATATGTTCTAAATGTCAACCATCGTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMLIRFRSKNGTHRVSCQENDLFGTVIE
KLVGNLDPNADVDTFTVCEKPGQGIHAVSELADRTVMDLGLKHGDMLILN
YSDKPANEKDGVNVEIGSVGIDSKGIRQHRYGPLRIKELAVDEELEKEDG
LIPRQKSKLCKHGDRGMCEYCSPLPPWDKEYHEKNKIKHISFHSYLKKLN
ENANKKENGSSYISPLSEPDFRINKRCHNGHEPWPRGICSKCQPS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU49736
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee