cDNA Clone FYCO1

• Synonyms: CATC2; RUFY3; ZFYVE7
• Plasmid: pBabeD hygro FLAG FYCO1
• Parent Plasmid: pBabeD hygro FLAG
• Expressed: FLAG-FYCO1
• Genbank: NM_024513.3 GI:364502962 with silent change c924a
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCTATGGCCTCCACCAATGCAGAGAGCCAGCTCCAGAGAATCATCCGAGACTTGCAAGATGCTGTGACAGAACTAAGCAAAGAATTTCAGGAAGCAGGGGAACCCATCACGGATGACAGCACCAGCTTGCATAAATTTTCTTATAAACTTGAGTATCTCCTGCAATTTGATCAGAAAGAGAAGGCCACCCTCCTGGGCAACAAGAAGGACTACTGGGATTACTTCTGTGCCTGCCTGGCCAAGGTGAAAGGAGCCAATGATGGGATCCGCTTTGTCAAGTCTATCTCAGAGCTCCGAACATCCTTGGGGAAAGGAAGAGCATTTATTCGCTACTCCTTGGTGCACCAGAGGTTGGCAGACACCTTACAGCAGTGCTTCATGAACACCAAAGTGACCAGTGACTGGTACTATGCAAGAAGCCCCTTTCTGCAGCCAAAGCTGAGCTCGGACATTGTGGGCCAACTCTATGAGCTGACTGAGGTTCAGTTTGACCTGGCGTCGAGGGGCTTTGACTTGGATGCTGCCTGGCCAACATTTGCCAGGAGGACGCTGACCACTGGCTCTTCTGCTTACCTGTGGAAACCCCCTAGCCGCAGCTCCAGCATGAGCAGCTTGGTGAGCAGCTACCTGCAGACTCAAGAGATGGTGTCCAACTTTGACCTGAACAGCCCCCTAAACAACGAGGCATTGGAGGGCTTTGATGAGATGCGACTAGAGCTGGACCAGTTGGAGGTGCGGGAGAAGCAGCTACGGGAGCGCATGCAGCAGCTGGACAGAGAGAACCAGGAGCTGAGGGCAGCTGTCAGCCAGCAAGGGGAGCAACTGCAGACAGAGAGGGAGAGGGGGCGCACTGCAGCGGAGGACAACGTTCGCCTCACTTGCTTGGTAGCTGAGCTCCAGAAGCAGTGGGAGGTCACCCAGGCCACACAGAACACTGTGAAGGAGCTGCAGACATGCCTGCAGGGCCTGGAGCTAGGAGCAGCAGAGAAGGAGGAGGACTACCACACAGCCCTGCGGCGGCTGGAGTCCATGCTGCAGCCCTTGGCACAGGAGCTTGAGGCCACACGGGACTCACTGGACAAGAAAAACCAGCATTTAGCCAGCTTCCCAGGCTGGCTAGCCATGGCTCAGCAGAAGGCAGATACGGCATCAGACACAAAGGGCCGGCAAGAACCTATTCCCAGTGATGCGGCCCAGGAGATGCAGGAGCTAGGGGAGAAGCTTCAAGCCCTAGAAAGGGAGAGAACCAAGGTCGAGGAGGTCAACAGACAGCAGAGTGCCCAACTGGAACAGCTGGTCAAGGAGCTTCAGCTGAAAGAGGATGCCCGGGCCAGCCTGGAGCGCCTGGTGAAGGAGATGGCCCCACTCCAGGAGGAGTTGTCTGGGAAGGGACAGGAGGCAGACCAGCTCTGGCGACGGCTGCAGGAGTTGCTGGCCCACACGAGCTCCTGGGAGGAGGAGCTAGCAGAGTTGAGGCGGGAGAAAAAACAGCAACAGGAGGAGAAGGAGCTGCTGGAGCAGGAGGTCAGGTCTCTGACCCGGCAGCTGCAGTTCCTGGAGACCCAGCTGGCACAGGTGAGCCAACATGTGAGTGACCTGGAGGAGCAGAAGAAGCAGCTCATTCAGGACAAAGACCACCTCAGCCAGCAGGTGGGTATGCTCGAGCGGCTTGCTGGGCCGCCTGGCCCAGAACTGCCAGTGGCAGGTGAGAAGAATGAGGCCCTGGTCCCTGTGAACTCCAGTCTGCAAGAGGCCTGGGGGAAGCCAGAGGAGGAGCAGAGGGGCCTGCAGGAGGCACAGTTAGACGATACCAAGGTGCAAGAGGGCAGCCAGGAGGAAGAGCTCCGGCAGGCCAACAGGGAGCTGGAGAAGGAGCTACAGAATGTGGTCGGGCGTAACCAGCTCCTGGAGGGCAAGCTGCAAGCCCTGCAGGCCGATTACCAGGCTTTGCAGCAGCGGGAATCAGCCATCCAGGGCTCCTTGGCCTCCCTGGAGGCCGAGCAGGCCAGCATCCGGCACTTGGGTGACCAGATGGAGGCGAGCTTGCTGGCTGTAAGGAAGGCCAAGGAGGCCATGAAAGCCCAGATGGCAGAGAAGGAGGCCATTCTACAGAGCAAGGAGGGCGAGTGTCAGCAGCTGCGGGAGGAGGTGGAGCAGTGCCAGCAACTGGCAGAAGCCCGGCACAGAGAGCTTAGGGCTCTCGAGAGCCAGTGCCAGCAGCAGACCCAGCTGATTGAGGTCCTCACAGCAGAGAAAGGCCAACAGGGAGTTGGCCCACCCACTGACAATGAAGCCCGTGAGCTGGCTGCCCAGCTAGCCCTGTCTCAGGCGCAGCTGGAAGTCCATCAGGGGGAGGTCCAACGGCTGCAGGCTCAGGTGGTGGACCTCCAGGCCAAGATGCGGGCAGCCCTGGATGACCAGGACAAGGTGCAGAGCCAGCTAAGCATGGCTGAGGCCGTCCTGAGGGAGCACAAAACCCTTGTGCAGCAGCTGAAGGAGCAGAATGAAGCCCTTAACAGAGCCCATGTCCAGGAGCTGCTGCAATGCTCGGAGCGTGAAGGGGCACTGCAGGAGGAGAGGGCCGATGAGGCCCAGCAGAGGGAGGAGGAGCTGCGGGCCCTGCAGGAGGAGCTGTCCCAGGCCAAATGCAGCTCCGAGGAAGCACAGCTGGAGCACGCTGAGCTGCAAGAGCAGCTGCACCGGGCCAACACAGACACAGCTGAGCTGGGCATCCAGGTTTGCGCACTGACCGTGGAAAAGGAGCGAGTGGAGGAGGCACTGGCCTGTGCTGTCCAGGAGCTCCAGGACGCCAAAGAGGCAGCCTCAAGGGAGCGAGAGGGCCTGGAGCGCCAAGTAGCTGGGCTGCAGCAAGAGAAGGAGAGCTTGCAGGAGAAGCTGAAGGCGGCCAAGGCAGCAGCCGGCTCACTGCCTGGCCTGCAGGCCCAGCTCGCCCAGGCAGAGCAGCGGGCCCAGAGCCTCCAAGAGGCTGCACACCAGGAGCTCAACACCCTCAAGTTCCAGCTGAGTGCTGAAATCATGGACTACCAGAGCAGACTTAAGAATGCTGGTGAAGAGTGCAAGAGCCTCAGGGGCCAGCTTGAGGAGCAAGGCCGGCAGCTGCAGGCTGCTGAGGAAGCTGTGGAGAAGCTGAAGGCCACCCAAGCAGACATGGGAGAGAAGCTGAGCTGCACTAGCAACCATCTTGCAGAGTGCCAGGCGGCCATGCTGAGGAAGGACAAGGAGGGGGCTGCCCTGCGTGAAGACCTAGAAAGGACCCAGAAGGAACTCGAAAAAGCCACAACAAAAATCCAAGAGTATTACAACAAACTCTGCCAGGAGGTGACAAATCGTGAGAGGAATGACCAGAAGATGCTTGCTGACCTGGATGACCTCAACAGAACCAAGAAGTATCTCGAGGAGCGGCTGATAGAGCTGCTCAGGGACAAGGATGCTCTCTGGCAGAAGTCAGATGCCCTGGAATTCCAGCAGAAGCTCAGTGCTGAGGAGAGATGGCTCGGAGACACAGAGGCAAACCACTGCCTCGACTGTAAGCGGGAGTTCAGCTGGATGGTGCGGCGGCACCACTGCAGGATATGTGGCCGCATCTTCTGTTACTACTGCTGCAACAACTACGTCCTGAGCAAGCACGGTGGCAAAAAGGAGCGCTGCTGCCGAGCCTGTTTCCAGAAGCTCAGTGAAGGCCCTGGCTCCCCTGATAGCAGTGGCTCAGGCACTAGCCAGGGAGAGCCCAGCCCTGCACTGTCACCAGCCTCACCTGGGCCCCAGGCCACAGGAGGCCAAGGAGCAAATACAGACTACAGGCCACCGGACGACGCTGTGTTTGATATCATCACAGATGAGGAATTGTGCCAGATACAGGAGTCCGGCTCCTCTTTGCCTGAAACACCCACTGAAACTGATTCTCTCGACCCAAATGCGGCTGAACAGGATACTACATCAACCTCGCTAACGCCTGAGGACACTGAAGACATGCCCGTGGGGCAGGATTCGGAAATCTGCCTGCTGAAGTCTGGAGAACTGATGATCAAAGTACCCCTCACAGTGGATGAGATCGCCAGCTTCGGGGAGGGTAGCAGGGAGCTGTTTGTGAGGTCCAGCACCTACAGCCTGATCCCCATCACTGTGGCCGAGGCAGGCCTCACCATCAGCTGGGTCTTCTCCTCTGACCCCAAGAGCATCTCCTTCAGTGTGGTCTTCCAGGAGGCCGAGGACACACCGCTGGATCAGTGTAAGGTCCTCATTCCCACGACCCGATGCAACTCCCACAAGGAGAACATCCAGGGCCAGCTCAAGGTTCGCACACCCGGCATCTACATGCTCATCTTCGACAATACCTTCTCAAGGTTTGTCTCTAAAAAGGTATTTTATCACTTGACGGTTGATCGGCCTGTGATCTACGATGGAAGTGATTTCCTGTAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMASTNAESQLQRIIRDLQDAVTELSKEFQEAGEPITDDS
TSLHKFSYKLEYLLQFDQKEKATLLGNKKDYWDYFCACLAKVKGANDGIR
FVKSISELRTSLGKGRAFIRYSLVHQRLADTLQQCFMNTKVTSDWYYARS
PFLQPKLSSDIVGQLYELTEVQFDLASRGFDLDAAWPTFARRTLTTGSSA
YLWKPPSRSSSMSSLVSSYLQTQEMVSNFDLNSPLNNEALEGFDEMRLEL
DQLEVREKQLRERMQQLDRENQELRAAVSQQGEQLQTERERGRTAAEDNV
RLTCLVAELQKQWEVTQATQNTVKELQTCLQGLELGAAEKEEDYHTALRR
LESMLQPLAQELEATRDSLDKKNQHLASFPGWLAMAQQKADTASDTKGRQ
EPIPSDAAQEMQELGEKLQALERERTKVEEVNRQQSAQLEQLVKELQLKE
DARASLERLVKEMAPLQEELSGKGQEADQLWRRLQELLAHTSSWEEELAE
LRREKKQQQEEKELLEQEVRSLTRQLQFLETQLAQVSQHVSDLEEQKKQL
IQDKDHLSQQVGMLERLAGPPGPELPVAGEKNEALVPVNSSLQEAWGKPE
EEQRGLQEAQLDDTKVQEGSQEEELRQANRELEKELQNVVGRNQLLEGKL
QALQADYQALQQRESAIQGSLASLEAEQASIRHLGDQMEASLLAVRKAKE
AMKAQMAEKEAILQSKEGECQQLREEVEQCQQLAEARHRELRALESQCQQ
QTQLIEVLTAEKGQQGVGPPTDNEARELAAQLALSQAQLEVHQGEVQRLQ
AQVVDLQAKMRAALDDQDKVQSQLSMAEAVLREHKTLVQQLKEQNEALNR
AHVQELLQCSEREGALQEERADEAQQREEELRALQEELSQAKCSSEEAQL
EHAELQEQLHRANTDTAELGIQVCALTVEKERVEEALACAVQELQDAKEA
ASREREGLERQVAGLQQEKESLQEKLKAAKAAAGSLPGLQAQLAQAEQRA
QSLQEAAHQELNTLKFQLSAEIMDYQSRLKNAGEECKSLRGQLEEQGRQL
QAAEEAVEKLKATQADMGEKLSCTSNHLAECQAAMLRKDKEGAALREDLE
RTQKELEKATTKIQEYYNKLCQEVTNRERNDQKMLADLDDLNRTKKYLEE
RLIELLRDKDALWQKSDALEFQQKLSAEERWLGDTEANHCLDCKREFSWM
VRRHHCRICGRIFCYYCCNNYVLSKHGGKKERCCRACFQKLSEGPGSPDS
SGSGTSQGEPSPALSPASPGPQATGGQGANTDYRPPDDAVFDIITDEELC
QIQESGSSLPETPTETDSLDPNAAEQDTTSTSLTPEDTEDMPVGQDSEIC
LLKSGELMIKVPLTVDEIASFGEGSRELFVRSSTYSLIPITVAEAGLTIS
WVFSSDPKSISFSVVFQEAEDTPLDQCKVLIPTTRCNSHKENIQGQLKVR
TPGIYMLIFDNTFSRFVSKKVFYHLTVDRPVIYDGSDFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49762
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee