cDNA Clone NCS2

• Synonyms: YNL119W; TUC2
• Plasmid: pRS413-ADH Pr-2xStrep-FLAG-NCS2-Cyc1 ter
• Parent Plasmid: pRS413 pADH 2xStrep-FLAG
• Expressed: 2xStrep-FLAG-NCS2
• Genbank: NC_001146.8
• Tag: FLAG
• Tag: StrepII
• Species: Saccharomyces cerevisiae

Sequence of Insert

GAATTCATGGAGTGCCAGAGGTGCCCTGCATCTGCCAGAAATCCAGCGAC
AGTTGAGTCTAGAAAGGAAAAGTTTTGCGATGAATGTTTCATCAAGTTTG
TTAGTACTAAACAAAGGAAACAAATGATGAAGGATGAATATTTCAGGAAC
CTATTTAAAGTTATATACCCATTTGAAAAAGAAGGATCCGTCTCTAAGAT
TCTGCTCCCCTTATCACATTCTGATTCAGGATCTCTGGTTATGTTAGATA
TTGTGCACGACTTGCTATTGGAACAAACTAAGCAACACAATAATCGTACA
GGCTTTACCGTCGATGTACTCACGGTATTCACCGAGGAAAATGTTTCTGT
TATCAAAGAACGTATGGAATCCTTGATTAATGAAAAAATGTCACAACTGA
ATAAAATATCCAACATATTTAATGTCCATTTTATTGATGTTAACGAATTT
TTCAACAATGCATCGGAAGTATCGACTTTTATCATTGACAATGAAAATTT
TGAGATTTTCAGCAAGTCAAAGTCAGTAGATGATAGCAATATATTAACAT
TAAAAGAAATATTAGGCAAATACTGTCTCAATAATTCATCCAGGTCTGAT
TTAATATCGATTATAAAAACTCAATTGATCAAGCATTTTGCATACGAAAA
TGGTTATAATGCCATAATGTGGGGACATTCCATGACAAAGCTATCTGAAG
TAATTATTTCTCTTGTGGTAAAAGGTAAAGGTTCTCAAATAGCGACGTTT
CTAGACTCGGAATCATTTGATACTTTGAATAATAAACCATGTAAGTATAA
AAACCTATATCCTATGAAAGATCTATTATCCGTCGAGATTGAAAGTTTTT
TACAAATCAGAAATCTGGCACAGTTCCTCATTAACGTTGAAGAAACTAAT
GTGAAGCCTAATTGCTTGATCGCCAGAAAATCACTGCCGTCACTTGGTCA
ACAAAAATTGGTAAAAAATATGACAATCAACGAAATTACCAACAAATACT
TTCAGGATATTCAGAACGATTATTCAAATATAATATCGACAGTTTTAAGA
ACCGCAGATAAATTGACGCAACCTAAATCAAGTATGGCTAAGCCGTCACA
ATGTCAAATATGTCAATCTAAAATATACACAAATCCATCCAATTGGTTAA
ATAGGATCACAGTTACTTCGCCTTATCCTGTGGAGACCACTGAAGAAAAG
TATTTATTTAAACAGTGGCAAGATTCAAAATTAGGGCAATCTCACACACA
TTATGTTGAATTGTTGAATGAAATCAAACAGGGTGCCAGTAATAGCCTCG
ATGTTGAAGACGGTGATGTCAAATTGTGTTATGGTTGTTTAATCTTATTG
AATACCAGTATCAAGGATAAGAATTTGGTTTGGCCAAAAGTCGATACCAT
GGATATTACGGCAAATGCGACCAATAAAAACAAAGAATTGAGCCAAATAT
TGGATCAGTTCGAGATTAATTCCGATGGGGAGGAATAAgtcgacCATGTA
ATTAGTTATGTCACGCTTACATTCACGCCCTCCTCCCACATCCGCTCTAA
CCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTT
TTTTAATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTT
CTTTTTTTTCTGTACAAACGCGTGTACGCATGTAACATTATACTGAAAAC
CTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTGGGCCC

Amino Acid Sequence

MWSHPQFEKWSHPQFEKGGGGGMDYKDDDDKEFMECQRCPASARNPATVE
SRKEKFCDECFIKFVSTKQRKQMMKDEYFRNLFKVIYPFEKEGSVSKILL
PLSHSDSGSLVMLDIVHDLLLEQTKQHNNRTGFTVDVLTVFTEENVSVIK
ERMESLINEKMSQLNKISNIFNVHFIDVNEFFNNASEVSTFIIDNENFEI
FSKSKSVDDSNILTLKEILGKYCLNNSSRSDLISIIKTQLIKHFAYENGY
NAIMWGHSMTKLSEVIISLVVKGKGSQIATFLDSESFDTLNNKPCKYKNL
YPMKDLLSVEIESFLQIRNLAQFLINVEETNVKPNCLIARKSLPSLGQQK
LVKNMTINEITNKYFQDIQNDYSNIISTVLRTADKLTQPKSSMAKPSQCQ
ICQSKIYTNPSNWLNRITVTSPYPVETTEEKYLFKQWQDSKLGQSHTHYV
ELLNEIKQGASNSLDVEDGDVKLCYGCLILLNTSIKDKNLVWPKVDTMDI
TANATNKNKELSQILDQFEINSDGEE*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU24542
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: parental 24514