cDNA Clone NCS2

• Synonyms: YNL119W; TUC2
• Plasmid: pRS413-ADH Pr-3HA-NCS6-Cyc1 ter; ADH Pr-3HA-NCS2-Cyc1 ter
• Parent Plasmid: pRS413 pADH 3HA
• Expressed: 3HA-NCS2
• Genbank: NC_001146.8
• Tag: 3HA
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGGCCCggtggaggcggtggaggcGCCGGGATCGAAGAAATGATGGTAAA
TGAAATAGGAAATCAAGGAGCATGAAGGCAAAAGACAAATATAAGGGTCG
AACGAAAAATAAAGTGAAAAGTGTTGATATGATGTATTTGGCTTTGCGGC
GCCGAAAAAACGAGTTTACGCAATTGCACAATCATGCTGACTCTGTGGCG
GACCCGCGCTCTTGCCGGCCCGGCGATAACGCTGGGCGTGAGGCTGTGCC
CGGCGGAGTTTTTTGCGCCTGCATTTTCCAAGGTTTACCCTGCGCTAAGG
GGCGAGATTGGAGAAGCAATAAGAATGCCGGTTGGGGTTGCGATGATGAC
GACCACGACAACTGGTGTCATTATTTAAGTTGCCGAAAGAACCTGAGTGC
ATTTGCAACATGAGTATACTAGAAGAATGAGCCAAGACTTGCGAGACGCG
AGTTTGCCGGTGGTGCGAACAATAGAGCGACCATGACCTTGAAGGTGAGA
CGCGCATAACCGCTAGAGTACTTTGAAGAGGAAACAGCAATAGGGTTGCT
ACCAGTATAAATAGACAGGTACATACAACACTGGAAATGGTTGTCTGTTT
GAGTACGCTTTCAATTCATTTGGGTGTGCACTTTATTATGTTACAATATG
GAAGGGAACTTTACACTTCTCCTATGCACATATATTAATTAAAGTCCAAT
GCTAGTAGAGAAGGGGGGTAACACCCCTCCGCGCTCTTTTCCGATTTTTT
TCTAAACCGTGGAATATTTCGGATATCCTTTTGTTGTTTCCGGGTGTACA
ATATGGACTTCCTCTTTTCTGGCAACCAAACCCATACATCGGGATTCCTA
TAATACCTTCGTTGGTCTCCCTAACATGTAGGTGGCGGAGGGGAGATATA
CAATAGAACAGATACCAGACAAGACATAATGGGCTAAACAAGACTACACC
AATTACACTGCCTCATTGATGGTGGTACATAACGAACTAATACTGTAGCC
CTAGACTTGATAGCCATCATCATATCGAAGTTTCACTACCCTTTTTCCAT
TTGCCATCTATTGAAGTAATAATAGGCGCATGCAACTTCTTTTCTTTTTT
TTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAA
AAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCA
ACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAA
ACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTA
TACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAAGTTTGCTG
TCTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCT
CGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATAT
TTCAAGCTATACCAAGCATACAATCAACTCCAAGCTGGCCGCCTAACCgg
atccATGTACCCATACGATGTGCCAGATTACGCCATGTACCCATACGATG
TGCCAGATTACGCCATGTACCCATACGATGTGCCAGATTACGCCgaattc
ATGGAGTGCCAGAGGTGCCCTGCATCTGCCAGAAATCCAGCGACAGTTGA
GTCTAGAAAGGAAAAGTTTTGCGATGAATGTTTCATCAAGTTTGTTAGTA
CTAAACAAAGGAAACAAATGATGAAGGATGAATATTTCAGGAACCTATTT
AAAGTTATATACCCATTTGAAAAAGAAGGATCCGTCTCTAAGATTCTGCT
CCCCTTATCACATTCTGATTCAGGATCTCTGGTTATGTTAGATATTGTGC
ACGACTTGCTATTGGAACAAACTAAGCAACACAATAATCGTACAGGCTTT
ACCGTCGATGTACTCACGGTATTCACCGAGGAAAATGTTTCTGTTATCAA
AGAACGTATGGAATCCTTGATTAATGAAAAAATGTCACAACTGAATAAAA
TATCCAACATATTTAATGTCCATTTTATTGATGTTAACGAATTTTTCAAC
AATGCATCGGAAGTATCGACTTTTATCATTGACAATGAAAATTTTGAGAT
TTTCAGCAAGTCAAAGTCAGTAGATGATAGCAATATATTAACATTAAAAG
AAATATTAGGCAAATACTGTCTCAATAATTCATCCAGGTCTGATTTAATA
TCGATTATAAAAACTCAATTGATCAAGCATTTTGCATACGAAAATGGTTA
TAATGCCATAATGTGGGGACATTCCATGACAAAGCTATCTGAAGTAATTA
TTTCTCTTGTGGTAAAAGGTAAAGGTTCTCAAATAGCGACGTTTCTAGAC
TCGGAATCATTTGATACTTTGAATAATAAACCATGTAAGTATAAAAACCT
ATATCCTATGAAAGATCTATTATCCGTCGAGATTGAAAGTTTTTTACAAA
TCAGAAATCTGGCACAGTTCCTCATTAACGTTGAAGAAACTAATGTGAAG
CCTAATTGCTTGATCGCCAGAAAATCACTGCCGTCACTTGGTCAACAAAA
ATTGGTAAAAAATATGACAATCAACGAAATTACCAACAAATACTTTCAGG
ATATTCAGAACGATTATTCAAATATAATATCGACAGTTTTAAGAACCGCA
GATAAATTGACGCAACCTAAATCAAGTATGGCTAAGCCGTCACAATGTCA
AATATGTCAATCTAAAATATACACAAATCCATCCAATTGGTTAAATAGGA
TCACAGTTACTTCGCCTTATCCTGTGGAGACCACTGAAGAAAAGTATTTA
TTTAAACAGTGGCAAGATTCAAAATTAGGGCAATCTCACACACATTATGT
TGAATTGTTGAATGAAATCAAACAGGGTGCCAGTAATAGCCTCGATGTTG
AAGACGGTGATGTCAAATTGTGTTATGGTTGTTTAATCTTATTGAATACC
AGTATCAAGGATAAGAATTTGGTTTGGCCAAAAGTCGATACCATGGATAT
TACGGCAAATGCGACCAATAAAAACAAAGAATTGAGCCAAATATTGGATC
AGTTCGAGATTAATTCCGATGGGGAGGAATAAgtcgacCATGTAATTAGT
TATGTCACGCTTACATTCACGCCCTCCTCCCACATCCGCTCTAACCGAAA
AGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTTAA
TAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTT
TTTCTGTACAAACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCT
TGAGAAGGTTTTGGGACGCTCGAAGGCTTTggtggaggcggtggaggcGG
GCCC

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFMECQRCPASARNPATVES
RKEKFCDECFIKFVSTKQRKQMMKDEYFRNLFKVIYPFEKEGSVSKILLP
LSHSDSGSLVMLDIVHDLLLEQTKQHNNRTGFTVDVLTVFTEENVSVIKE
RMESLINEKMSQLNKISNIFNVHFIDVNEFFNNASEVSTFIIDNENFEIF
SKSKSVDDSNILTLKEILGKYCLNNSSRSDLISIIKTQLIKHFAYENGYN
AIMWGHSMTKLSEVIISLVVKGKGSQIATFLDSESFDTLNNKPCKYKNLY
PMKDLLSVEIESFLQIRNLAQFLINVEETNVKPNCLIARKSLPSLGQQKL
VKNMTINEITNKYFQDIQNDYSNIISTVLRTADKLTQPKSSMAKPSQCQI
CQSKIYTNPSNWLNRITVTSPYPVETTEEKYLFKQWQDSKLGQSHTHYVE
LLNEIKQGASNSLDVEDGDVKLCYGCLILLNTSIKDKNLVWPKVDTMDIT
ANATNKNKELSQILDQFEINSDGEE*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU24532
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 24515 into 24144 Apa1 to create dual expression vector