cDNA Clone DHX57

• Synonyms: DDX57
• Plasmid: pSC-B DHX57
• Parent Plasmid: pSC-B
• Genbank: NM_198963.1
• Species: Human

Sequence of Insert

GCTAGCATGAGTTCTTCAGTAAGAAGAAAAGGCAAGCCAGGCAAAGGAGGTGGAAAAGGGTCTTCTAGAGGAGGAAGAGGAGGCAGGAGTCACGCCAGTAAATCTCATGGGAGTGGTGGCGGTGGCGGTGGTGGTGGTGGTGGAGGTGGCGGCAACAGAAAGGCCTCCAGTAGAATATGGGATGATGGAGATGACTTTTGTATCTTCAGTGAATCAAGGCGCCCTTCCAGACCTAGCAACAGTAACATAAGCAAAGGAGAGTCACGCCCAAAATGGAAACCCAAAGCCAAAGTACCCCTTCAGACTCTACATATGACTTCTGAGAATCAAGAGAAAGTGAAAGCTCTTCTCCGAGACCTGCAAGAACAAGATGCTGATGCTGGATCTGAAAGAGGCCTTTCTGGGGAGGAGGAAGATGATGAGCCTGATTGCTGTAACGATGAGCGGTACTGGCCAGCTGGACAGGAACCTTCCCTCGTTCCCGACTTGGATCCTTTGGAATATGCTGGCTTAGCCTCAGTGGAGCCTTATGTTCCAGAATTTACAGTCTCCCCATTTGCAGTGCAAAAACTTTCCAGGTATGGTTTCAATACTGAACGCTGTCAAGCGGTCCTGAGGATGTGTGATGGAGATGTGGGAGCATCACTAGAGCATCTCCTTACCCAGTGTTTTTCAGAGACATTTGGAGAGAGGATGAAGATCTCTGAGGCAGTCAACCAGATAAGCTTGGATGAGTGTATGGAACAGCGACAGGAAGAGGCATTTGCTCTCAAGTCCATCTGTGGAGAAAAATTTATAGAAAGAATTCAGAACAGAGTCTGGACCATTGGGTTAGAACTGGAGTATCTGACAAGTAGATTCCGCAAATCCAAGCCAAAAGAAAGTACCAAAAATGTACAAGAGAATTCACTTGAAATCTGTAAATTTTACCTCAAAGGAAATTGTAAATTTGGATCAAAATGCAGATTCAAACATGAAGTGCCCCCAAATCAAATTGTTGGAAGAATAGAAAGAAGTGTAGATGATTCTCATCTTAATGCTATTGAAGATGCATCTTTTTTATATGAACTTGAAATTCGATTTTCTAAAGACCACAAATATCCCTACCAAGCTCCGCTCGTGGCATTTTATTCCACCAATGAGAACCTACCTCTGGCTTGTCGTTTACATATTTCTGAGTTTCTTTATGACAAGGCCTTGACATTTGCGGAAACTTCGGAACCTGTCGTATATTCTTTGATAACCCTTTTAGAGGAAGAGTCGGAAATAGTCAAGTTACTAACGAATACCCACCACAAGTATAGTGACCCTCCTGTGAACTTTCTGCCAGTACCCTCTAGGACCAGAATAAATAATCCTGCCTGTCATAAAACAGTGATTCCAAATAATTCTTTTGTTTCTAATCAAATTCCAGAAGTTGAAAAAGCATCAGAATCTGAGGAGTCAGATGAGGATGACGGTCCTGCACCTGTTATAGTAGAGAATGAAAGCTATGTGAACCTTAAGAAAAAGATTTCCAAAAGATATGACTGGCAGGCAAAGTCAGTACATGCTGAAAATGGTAAAATCTGCAAGCAGTTCCGAATGAAACAGGCTTCCAGACAGTTCCAGTCCATTCTGCAAGAGAGGCAATCACTCCCTGCTTGGGAAGAAAGAGAAACCATTCTTAACTTATTGCGTAAGCACCAGGTGGTTGTCATAAGTGGTATGACTGGATGTGGGAAAACCACACAAATTCCGCAGTTTATTCTGGATGATTCTCTGAATGGACCACCTGAGAAGGTAGCCAACATCATCTGTACCCAACCCCGACGAATCTCTGCAATCTCTGTTGCTGAACGCGTTGCTAAAGAAAGAGCAGAGAGGGTGGGTCTGACCGTGGGATACCAGATTCGGTTAGAAAGTGTCAAGTCCTCAGCCACCAGACTGTTATACTGCACCACGGGAGTGCTGCTGAGAAGGCTAGAAGGAGATACAGCTCTACAAGGAGTTTCCCATATCATTGTTGATGAAGTTCATGAGAGGACAGAAGAAAGTGACTTCTTGCTGCTAGTTTTGAAGGACATTGTATCGCAGAGGCCAGGTCTTCAAGTTATTTTAATGAGTGCAACTCTAAACGCTGAGCTTTTTTCAGACTATTTTAATTCCTGCCCCGTTATTACTATACCAGGTCGTACATTTCCTGTTGATCAATTTTTTTTGGAAGATGCAATTGCTGTGACAAGGTATGTATTACAGGATGGGAGCCCATATATGCGGTCCATGAAACAGATTTCAAAGGAAAAGCTTAAAGCAAGGCGGAACAGAACTGCATTTGAAGAAGTGGAAGAAGACCTAAGGCTCTCCCTTCACCTCCAGGATCAGGATTCTGTCAAAGATGCAGTGCCAGATCAACAGTTAGATTTTAAGCAGCTCCTGGCCCGCTATAAAGGGGTTAGCAAGTCAGTCATCAAAACAATGTCCATCATGGATTTTGAAAAGGTGAATCTTGAATTAATAGAGGCCTTATTAGAGTGGATTGTGGATGGAAAGCACTCCTACCCTCCAGGTGCTATACTTGTATTTTTACCAGGACTAGCAGAAATCAAAATGCTTTATGAACAGCTACAGTCTAATTCTCTTTTCAACAACAGACGTAGTAATCGATGTGTTATTCACCCACTTCATTCATCTTTATCCAGTGAAGAGCAGCAGGCTGTGTTTGTAAAACCTCCTGCAGGAGTAACTAAGATTATAATTTCCACCAACATTGCTGAGACATCCATAACCATCGATGATGTTGTCTATGTTATCGATTCTGGGAAAATGAAAGAAAAGAGATATGATGCCAGCAAAGGGATGGAAAGTCTAGAGGACACCTTTGTATCTCAAGCTAATGCTCTACAAAGGAAAGGCCGAGCAGGCCGTGTTGCATCTGGGGTCTGCTTCCATTTATTCACTAGCCATCACTACAATCACCAGCTTTTAAAACAACAGCTACCAGAAATACAAAGAGTGCCATTGGAACAGCTGTGTCTAAGAATTAAAATTTTAGAGATGTTTAGTGCTCATAATCTCCAGTCTGTGTTCTCTCGGCTCATTGAACCTCCACACACCGATTCTCTTCGTGCCTCAAAAATACGATTACGAGACTTAGGAGCATTAACTCCAGATGAAAGATTGACCCCTCTTGGGTATCATTTGGCCTCTCTGCCCGTGGATGTGAGAATTGGCAAACTAATGTTGTTTGGGTCTATCTTCCGCTGTTTGGATCCTGCTCTCACCATTGCTGCCAGTTTGGCTTTTAAGTCGCCGTTTGTATCTCCCTGGGATAAAAAAGAAGAAGCTAACCAGAAAAAGCTGGAATTTGCATTCGCAAACAGTGATTATCTGGCCCTTCTACAAGCGTATAAGGGATGGCAGCTAAGTACAAAAGAAGGCGTGCGTGCAAGTTATAATTACTGCAGACAAAACTTCTTGTCTGGAAGAGTTCTGCAGGAAATGGCCAGCCTCAAACGACAATTCACGGAACTGTTATCGGATATAGGGTTTGCAAGGGAAGGGCTCAGAGCAAGGGAAATTGAGAAAAGGGCCCAAGGAGGAGATGGTGTCTTAGATGCCACAGGAGAAGAGGCAAACTCAAATGCTGAGAACCCCAAGCTGATATCAGCAATGCTGTGTGCTGCTTTGTATCCAAATGTAGTGCAGGTGAAAAGCCCAGAAGGAAAATTTCAGAAGACCAGTACTGGAGCTGTCAGAATGCAACCAAAATCAGCTGAGTTGAAGTTTGTCACCAAGAACGATGGATATGTACACATTCACCCTTCATCAGTGAACTATCAGGTGAGACACTTTGACAGCCCCTACCTGTTGTACCACGAGAAGATAAAAACTAGTCGAGTATTCATCCGAGACTGCAGCATGGTGTCTGTGTACCCGCTGGTCTTGTTTGGAGGAGGCCAAGTGAATGTGCAGCTTCAAAGAGGAGAGTTCGTTGTCTCCCTGGATGATGGTTGGATCCGTTTTGTAGCTGCTTCCCATCAGGTGGCTGAACTGGTAAAGGAGCTTCGTTGTGAACTTGATCAGCTTCTCCAGGATAAAATTAAAAACCCAAGCATTGATCTGTGTACGTGTCCTCGAGGATCCCGGATCATCAGCACAATTGTGAAACTTGTCACCACACAAGCGGCCGC

Amino Acid Sequence

MSSSVRRKGKPGKGGGKGSSRGGRGGRSHASKSHGSGGGGGGGGGGGGGNRKASSRIWDDGDDFCIFSESRRPSRPSNSNISKGESRPKWKPKAKVPLQTLHMTSENQEKVKALLRDLQEQDADAGSERGLSGEEEDDEPDCCNDERYWPAGQEPSLVPDLDPLEYAGLASVEPYVPEFTVSPFAVQKLSRYGFNTERCQAVLRMCDGDVGASLEHLLTQCFSETFGERMKISEAVNQISLDECMEQRQEEAFALKSICGEKFIERIQNRVWTIGLELEYLTSRFRKSKPKESTKNVQENSLEICKFYLKGNCKFGSKCRFKHEVPPNQIVGRIERSVDDSHLNAIEDASFLYELEIRFSKDHKYPYQAPLVAFYSTNENLPLACRLHISEFLYDKALTFAETSEPVVYSLITLLEEESEIVKLLTNTHHKYSDPPVNFLPVPSRTRINNPACHKTVIPNNSFVSNQIPEVEKASESEESDEDDGPAPVIVENESYVNLKKKISKRYDWQAKSVHAENGKICKQFRMKQASRQFQSILQERQSLPAWEERETILNLLRKHQVVVISGMTGCGKTTQIPQFILDDSLNGPPEKVANIICTQPRRISAISVAERVAKERAERVGLTVGYQIRLESVKSSATRLLYCTTGVLLRRLEGDTALQGVSHIIVDEVHERTEESDFLLLVLKDIVSQRPGLQVILMSATLNAELFSDYFNSCPVITIPGRTFPVDQFFLEDAIAVTRYVLQDGSPYMRSMKQISKEKLKARRNRTAFEEVEEDLRLSLHLQDQDSVKDAVPDQQLDFKQLLARYKGVSKSVIKTMSIMDFEKVNLELIEALLEWIVDGKHSYPPGAILVFLPGLAEIKMLYEQLQSNSLFNNRRSNRCVIHPLHSSLSSEEQQAVFVKPPAGVTKIIISTNIAETSITIDDVVYVIDSGKMKEKRYDASKGMESLEDTFVSQANALQRKGRAGRVASGVCFHLFTSHHYNHQLLKQQLPEIQRVPLEQLCLRIKILEMFSAHNLQSVFSRLIEPPHTDSLRASKIRLRDLGALTPDERLTPLGYHLASLPVDVRIGKLMLFGSIFRCLDPALTIAASLAFKSPFVSPWDKKEEANQKKLEFAFANSDYLALLQAYKGWQLSTKEGVRASYNYCRQNFLSGRVLQEMASLKRQFTELLSDIGFAREGLRAREIEKRAQGGDGVLDATGEEANSNAENPKLISAMLCAALYPNVVQVKSPEGKFQKTSTGAVRMQPKSAELKFVTKNDGYVHIHPSSVNYQVRHFDSPYLLYHEKIKTSRVFIRDCSMVSVYPLVLFGGGQVNVQLQRGEFVVSLDDGWIRFVAASHQVAELVKELRCELDQLLQDKIKNPSIDLCTCPRGSRIISTIVKLVTTQ

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU25809
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: NO STOP for c terminal tagging. Has 2 silent mutations as compared to NCBI ref seq S1095, C1349; Final construct pcDNA5-FRT-TO-DHX57-GFP