cDNA Clone DNA2

• Plasmid: pcDNA5 FRT/TO GFP DNA2
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP DNA2
• Genbank: NM_001080449.2
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGAGCAGCTGAACGAACTGGAGCTGCTGATGGAGAAGAGTTTTTGGGAGGAGGCGGAGCTGCCGGCGGAGCTATTTCAGAAGAAAGTGGTAG CTTCCTTTCCAAGAACAGTTCTGAGCACAGGAATGGATAACCGGTACCTGGTGTTGGCAGTCAATACTGTACAGAACAAAGAGGGAAACTGTGAAAAGCG CCTGGTCATCACTGCTTCACAGTCACTAGAAAATAAAGAACTATGCATCCTTAGGAATGACTGGTGTTCTGTTCCAGTAGAGCCAGGAGATATCATTCAT TTGGAGGGAGACTGCACATCTGACACTTGGATAATAGATAAAGATTTTGGATATTTGATTCTATATCCAGACATGCTGATTTCTGGCACCAGCATAGCCA GTAGTATTCGATGTATGAGAAGAGCTGTCCTGAGTGAAACTTTTAGGAGCTCTGATCCAGCCACACGCCAAATGCTAATTGGTACGGTTCTCCATGAGGT GTTTCAAAAAGCAATAAATAATAGCTTTGCCCCAGAAAAGCTACAAGAACTTGCTTTTCAAACAATTCAAGAAATAAGACATTTGAAGGAAATGTACCGC TTAAATCTAAGTCAAGATGAAATAAAACAAGAAGTAGAGGACTATCTTCCTTCGTTTTGTAAATGGGCAGGAGATTTCATGCATAAAAACACTTCGACTG ACTTCCCTCAGATGCAGCTCTCTCTGCCAAGTGATAATAGTAAGGATAATTCAACATGTAACATTGAAGTCGTGAAACCAATGGATATTGAAGAAAGCAT TTGGTCCCCTAGGTTTGGATTGAAAGGCAAAATAGATGTTACAGTTGGTGTGAAAATACATCGAGGGTATAAAACAAAATACAAGATAATGCCGCTGGAA CTTAAAACTGGCAAAGAATCAAATTCTATTGAACACCGTAGTCAGGTTGTTCTGTACACTCTACTAAGCCAAGAGAGAAGAGCTGATCCAGAGGCTGGCT TGCTTCTCTACCTCAAGACTGGTCAGATGTACCCTGTGCCTGCCAACCATCTAGATAAAAGAGAATTATTAAAGCTAAGAAACCAGATGGCATTCTCATT GTTTCACCGTATTAGCAAATCTGCTACTAGACAGAAGACACAGCTTGCTTCTTTGCCACAAATAATTGAGGAAGAGAAAACTTGTAAATATTGTTCACAA ATTGGCAATTGTGCTCTTTATAGCAGAGCAGTTGAACAACAGATGGATTGTAGTTCAGTCCCAATTGTGATGCTGCCCAAAATAGAAGAAGAAACCCAGC ATCTGAAGCAAACACACTTAGAATATTTCAGCCTTTGGTGTCTAATGTTAACCCTGGAGTCACAATCGAAGGATAATAAAAAGAATCACCAAAATATCTG GCTAATGCCTGCTTCGGAAATGGAGAAGAGTGGCAGTTGCATTGGAAACCTGATTAGAATGGAACATGTAAAGATAGTTTGTGATGGGCAATATTTACAT AATTTCCAATGTAAACATGGTGCCATACCTGTCACAAATCTAATGGCAGGTGACAGAGTTATTGTAAGTGGAGAAGAAAGGTCACTGTTTGCTTTGTCTA GAGGATATGTGAAGGAGATTAACATGACAACAGTAACTTGTTTATTAGACAGAAACTTGTCGGTCCTTCCAGAATCAACTTTGTTCAGATTAGACCAAGA AGAAAAAAATTGTGATATAGATACCCCATTAGGAAATCTTTCCAAATTGATGGAAAACACGTTTGTCAGCAAAAAACTTCGAGATTTAATTATTGACTTT CGTGAACCTCAGTTTATATCCTACCTTAGTTCTGTTCTTCCACATGATGCAAAGGATACAGTTGCCTGCATTCTAAAGGGTTTGAATAAGCCTCAGAGGC AAGCGATGAAAAAGGTACTTCTTTCAAAAGACTACACACTCATCGTGGGTATGCCTGGGACAGGAAAAACAACTACGATATGTACTCTCGTAAGAATTCT CTACGCCTGTGGTTTTAGCGTTTTGTTGACCAGCTATACACACTCTGCTGTTGACAATATTCTTTTGAAGTTAGCCAAGTTTAAAATAGGATTTTTGCGT TTGGGTCAGATTCAGAAGGTTCATCCAGCTATCCAGCAATTTACAGAGCAAGAAATTTGCAGATCAAAGTCCATTAAATCCTTAGCTCTTCTAGAAGAAC TCTACAATAGTCAACTTATAGTTGCAACAACATGTATGGGAATAAACCATCCAATATTTTCCCGTAAAATTTTTGATTTTTGTATTGTGGATGAAGCCTC TCAAATTAGCCAACCAATTTGTCTGGGCCCCCTTTTTTTTTCACGGAGATTTGTGTTAGTGGGGGACCATCAGCAGCTTCCTCCCCTGGTGCTAAACCGT GAAGCAAGAGCTCTTGGCATGAGTGAAAGCTTATTCAAGAGGCTGGAGCAGAATAAGAGTGCTGTTGTACAGTTAACCGTGCAGTACAGAATGAACAGTA AAATTATGTCCTTAAGTAATAAGCTGACCTATGAGGGCAAGCTGGAGTGTGGATCAGACAAAGTGGCCAATGCAGTGATAAACCTACGTCACTTTAAAGA TGTGAAGCTGGAACTGGAATTTTATGCTGACTATTCTGATAATCCTTGGTTGATGGGAGTATTTGAACCCAACAATCCTGTTTGTTTCCTTAATACAGAC AAGGTTCCAGCGCCAGAACAAGTTGAAAAAGGTGGTGTGAGCAATGTAACAGAAGCCAAACTCATAGTTTTCCTAACCTCCATTTTTGTTAAGGCTGGAT GCAGTCCCTCTGATATTGGTATTATTGCACCGTACAGGCAGCAATTAAAGATCATCAATGATTTATTGGCACGTTCTATTGGGATGGTCGAAGTTAATAC AGTAGACAAATACCAAGGAAGGGACAAAAGTATTGTCCTAGTATCTTTTGTTAGAAGTAATAAGGATGGAACTGTTGGTGAACTCTTGAAAGATTGGCGA CGTCTTAATGTTGCTATAACCAGAGCCAAACATAAACTGATTCTTCTGGGGTGTGTGCCCTCACTAAATTGCTATCCTCCTTTGGAGAAGCTGCTTAATC ATTTAAACTCAGAAAAATTAATCATTGATCTTCCATCAAGAGAACATGAAAGTCTTTGCCACATATTGGGTGACTTTCAAAGAGAATAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMEQLNELELLMEKSFWEEAELPAELFQKKVVASFPRTVLSTGMDNRYLVLAVNTVQ
NKEGNCEKRLVITASQSLENKELCILRNDWCSVPVEPGDIIHLEGDCTSDTWIIDKDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRSSDPATRQM
LIGTVLHEVFQKAINNSFAPEKLQELAFQTIQEIRHLKEMYRLNLSQDEIKQEVEDYLPSFCKWAGDFMHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVV
KPMDIEESIWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRADPEAGLLLYLKTGQMYPVPANHLDKRELLK
LRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKD
NKKNHQNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPE
STLFRLDQEEKNCDIDTPLGNLSKLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTT
TICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKIF
DFCIVDEASQISQPICLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKSAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANA
VINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKVPAPEQVEKGGVSNVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLAR
SIGMVEVNTVDKYQGRDKSIVLVSFVRSNKDGTVGELLKDWRRLNVAITRAKHKLILLGCVPSLNCYPPLEKLLNHLNSEKLIIDLPSREHESLCHILGD
FQRE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46712
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: pcDNA5-FRT-TO-GFP-DNA2