cDNA Clone Cullin 1 (Xenopus)

• Plasmid: pET28a 6His-TEV Xenopus l. Cullin 1 1-412 V367R L371D
• Parent Plasmid: pET28a original
• Expressed: 6His-TEV Xenopus l. Cullin 1 1-412 V367R L371D
• Genbank: NP_001089364.1 with three silent changes
• Tag: 6His
• Species: Xenopus laevis

Sequence of Insert

TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGTTCCTCCCACCACCATCATCACCACGAAAATCTGTACTTCCAAGGTGGCTCTATGAGCAGCAACCGTAGCCAGAACCCGCATGGCCTGAAACAGATCGGTCTGGACCAGATCTGGGATGATCTGCGTGCGGGCATTCAGCAGGTGTACACCCGTCAGTCCATGGCTAAGTCTCGTTACATGGAGCTGTATACGCATGTGTACAACTACTGCACCTCTGTTCATCAATCCAACCAGGCTCGTGGCGCGGGTGTTCCGCCGAGCAAAAGCAAGAAGGGCCAGACCCCGGGCGGCGCGCAGTTCGTTGGTCTGGAACTGTACAAACGTCTGAAAGAATTCCTGAAAAATTACCTGACGAACCTGCTGAAAGATGGTGAAGACCTGATGGATGAATCCGTACTGAAATTCTATACTCAACAGTGGGAAGATTACCGTTTCTCCAGCAAAGTTCTGAACGGTATCTGCGCCTATCTGAACCGTCACTGGGTACGTCGTGAATGTGATGAAGGCCGCAAGGGTATCTACGAAATCTATTCCCTGGCGCTTGTTACCTGGCGTGACTGCCTGTTCAAGCCGCTGAACAAACAAGTTACCAACGCCGTACTGAAACTGATTGAAAAAGAACGCAACGGCGAGACCATCAACACTCGTCTGATTAGCGGTGTAGTGCAGAGCTACGTCGAACTGGGTCTGAACGAAGACGATGCGTTCGCTAAAGGCCCGACCCTGACTGTTTACAAAGAAAGCTTCGAAAGCCAGTTCCTGGCTGACACCGAACGTTTCTACACCCGTGAATCTACCGAATTTCTGCAGCAGAACCCGGTGACCGAATATATGAAAAAAGCCGAAGCGCGCCTGCTGGAAGAACAGCGTCGCGTTCAGGTTTATCTGCACGAATCCACTCAGGACGAGCTGGCGCGTAAATGTGAACAAGTTCTGATTGAAAAACACCTGGAAATCTTCCACACGGAGTTCCAAAACCTGCTGGACGCTGACAAAAACGAAGACCTGGGTCGCATGTATAACCTGGTGTCCCGCATCCAGGATGGCCTGGGTGAACTGAAGAAACTGCTGGAAACTCACATTCATAACCAGGGTCTGGCGGCAATCGAGAAGTGCGGCGAAGCAGCACAGAACGACCCGAAAATGTATCGCCAGACGGTAGATGATGTGCACAAAAAGTATAACGCACTGGTAATGTCCGCTTTCAACAACGATGCGGGCTTCGTCGCAGCACTGGACAAGGCGTGCGGTCGCTTCATTAACAACAACGCGGTGACCAAAATGGCCTAAGAATTCGAGCTCCGTCGAC

Amino Acid Sequence

MGSSHHHHHHENLYFQGGSMSSNRSQNPHGLKQIGLDQIWDDLRAGIQQV
YTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGA
QFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSK
VLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFKPLNKQVTN
AVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKES
FESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHE
STQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQD
GLGELKKLLETHIHNQGLAAIEKCGEAAQNDPKMYRQTVDDVHKKYNALV
MSAFNNDAGFVAALDKACGRFINNNAVTKMA*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU49867
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee