cDNA Clone OSR1

• Plasmid: pEBG HA OSR1 L473A
• Parent Plasmid: pEBG6P
• Expressed: GST-HA-OSR1 L473A
• Genbank: NM_145260.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCATGTACCCATACGATGTGCCAGATTACGCCATGTCCGAAGACTCGAGCGCCCTGCCCTGGTCCATCAACAGGGACGATTACGAGCTGCAGGAGGTGATCGGGAGTGGAGCAACTGCTGTAGTCCAAGCAGCTTATTGTGCCCCTAAAAAGGAGAAAGTGGCAATCAAACGGATAAACCTTGAGAAATGTCAAACTAGCATGGATGAACTCCTGAAAGAAATTCAAGCCATGAGTCAATGCCATCATCCTAATATTGTATCTTACTACACATCTTTTGTGGTAAAAGATGAGCTGTGGCTTGTCATGAAGCTGCTAAGTGGAGGTTCTGTTCTGGATATTATTAAGCACATTGTGGCAAAAGGGGAACACAAAAGTGGAGTCCTAGATGAATCTACCATTGCTACGATACTCCGAGAAGTACTGGAAGGGCTGGAATATCTGCATAAAAATGGACAGATCCACAGAGATGTGAAAGCTGGAAACATTCTTCTTGGAGAAGATGGCTCAGTACAGATTGCAGACTTTGGGGTTAGTGCTTTTTTAGCAACTGGTGGTGATATTACCCGAAATAAAGTGAGAAAGACCTTTGTTGGCACCCCTTGTTGGATGGCACCTGAAGTTATGGAACAGGTCCGTGGTTATGATTTCAAAGCTGATATTTGGAGTTTTGGAATTACAGCAATTGAATTGGCTACAGGGGCGGCTCCTTATCATAAATATCCACCAATGAAGGTTTTAATGCTGACACTGCAGAACGATCCTCCTTCTTTGGAAACTGGTGTTCAAGATAAAGAAATGCTGAAAAAATATGGAAAATCATTTAGAAAAATGATTTCATTGTGCCTTCAAAAAGATCCAGAAAAAAGACCAACAGCAGCAGAACTATTAAGGCACAAATTTTTCCAGAAAGCAAAGAATAAAGAATTTCTTCAAGAAAAAACATTGCAGAGAGCACCAACCATTTCTGAAAGAGCAAAAAAGGTTCGGAGGGTACCAGGTTCCAGTGGGCGTCTTCATAAGACAGAGGATGGAGGCTGGGAGTGGAGTGATGATGAATTTGATGAAGAAAGTGAGGAAGGGAAAGCAGCAATTTCACAACTCAGGTCTCCCCGAGTGAAAGAATCAATATCAAATTCTGAGCTCTTTCCAACAACTGATCCTGTGGGTACTTTGCTCCAAGTTCCAGAACAGATCTCTGCTCATCTACCTCAGCCAGCTGGGCAGATTGCTACACAGCCAACTCAAGTCTCTCTCCCACCCACCGCAGAGCCAGCAAAAACAGCTCAGGCTTTGTCTTCAGGATCAGGTTCACAAGAAACCAAGATCCCAATCAGTCTAGTACTAAGATTAAGGAATTCCAAAAAAGAACTAAATGATATTCGATTTGAATTTACTCCTGGGAGAGATACAGCAGAGGGTGTCTCTCAGGAACTCATTTCTGCTGGCGCGGTCGACGGAAGGGATTTAGTAATAGTGGCAGCTAATTTGCAGAAAATTGTGGAAGAACCTCAGTCAAATCGATCTGTCACTTTCAAACTGGCGTCTGGTGTCGAAGGCTCAGATATTCCTGATGATGGTAAACTGATAGGATTTGCCCAGCTCAGCATCAGCTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAMYPYDVPDYAMS
EDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEK
CQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLD
IIKHIVAKGEHKSGVLDESTIATILREVLEGLEYLHKNGQIHRDVKAGNI
LLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVR
GYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQ
DKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQE
KTLQRAPTISERAKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEGKA
AISQLRSPRVKESISNSELFPTTDPVGTLLQVPEQISAHLPQPAGQIATQ
PTQVSLPPTAEPAKTAQALSSGSGSQETKIPISLVLRLRNSKKELNDIRF
EFTPGRDTAEGVSQELISAGAVDGRDLVIVAANLQKIVEEPQSNRSVTFK
LASGVEGSDIPDDGKLIGFAQLSIS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48574
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: L473A