cDNA Clone HSN2

• Synonyms: WNK1
• Plasmid: pcDNA5frtTO GFP Wnk1 tv3 714-798 + HSN2 13-425
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-WNK1 tv3 (714-798)-HSN2 (13-425)
• Genbank: NM_020922.4
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCCCTCGTCGGGGCCGTAGCATGTCGGTTTGTGTTCCCATCTTTCTGCTGTTGCCTCTGTGTCCCGCATCTCTCCCAGTGCTCTTCCACCCCACCGCCAGTACTGTCTGCACCTCTTTCTCCTTCCCTCCTCCGGACTGCCCCGAGGAAACTTTTGCCGAAAAGCTTTCTAAAGCATTGGAGAGTGTCCTGCCTATGCACTCTGCCTCTCAGCGCAAGCACCGACGCTCCAGCCTGCCTTCCCTCTTTGTCAGTACTGGATCCGCCATGCCTCAGTCCATGGCGCATCCGTGTGGGGGGACCCCAACATACCCAGAATCACAGATATTTTTCCCAACTATTCATGAACGTCCAGTTTCTTTTTCACCACCTCCCACCTGCCCACCGAAAGTAGCCATTTCCCAGCGGCGTAAGAGCACCTCCTTCCTGGAAGCCCAAACTCACCACTTCCAACCCCTGCTGAGGACTGTTGGCCAAAGTCTTCTTCCACCTGGTGGCAGCCCAACTAACTGGACACCAGAGGCCGTAGTTATGTTGGGTACTACAGCCAGTAGAGTAACTGGAGAGTCATGTGAGATACAGGTCCATCCTATGTTTGAACCATCTCAAGTTTACAGTGACTATAGACCTGGACTAGTACTTCCAGAAGAAGCTCACTATTTTATTCCTCAGGAAGCAGTGTATGTAGCTGGGGTACATTACCAGGCCCGGGTGGCAGAACAGTATGAGGGCATTCCATACAACTCATCAGTACTGTCAAGTCCTATGAAACAGATACCTGAACAGAAGCCAGTACAAGGGGGCCCTACTTCAAGTTCTGTCTTTGAATTTCCATCTGGACAGGCTTTCCTGGTAGGACACCTTCAGAATCTAAGATTAGATTCTGGATTGGGTCCGGGATCTCCCCTCTCTAGTATTTCTGCACCTATCAGTACAGATGCTACACGTTTGAAATTTCACCCTGTCTTTGTTCCTCATTCTGCGCCTGCTGTGTTAACTCATAACAATGAGAGCAGAAGCAACTGTGTATTTGAATTTCATGTTCACACACCAAGCTCCTCTTCAGGAGAAGGAGGTGGAATCTTACCTCAGCGTGTTTACCGAAATCGGCAGGTTGCAGTGGACTTGAATCAAGAAGAACTGCCTCCTCAATCAGTTGGATTACATGGCTACTTGCAGCCTGTGACTGAAGAAAAGCATAATTACCATGCCCCAGAATTGACCGTTTCTGTGGTAGAGCCTATCGGACAGAACTGGCCAATAGGAAGCCCAGAATATTCCAGTGATTCCTCACAAATCACTTCTTCAGACCCCAGTGATTTTCAGTCACCTCCCCCTACAGGGGGAGCAGCTGCACCTTTTGGCTCTGACGTCTCAATGCCCTTTATCCATCTGCCTCAGACAGTGTTACAAGAATCCCCACTTTTCTTCTGTTTCCCCCAAGGAACCACATCTCAGCAGGTCTTAACTGCCTCATTTTCTTCAGGAGGATCTGCACTTCATCCACAGTAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGS
PRRGRSMSVCVPIFLLLPLCPASLPVLFHPTASTVCTSFSFPPPDCPEETFAEKLS
KALESVLPMHSASQRKHRRSSLPSLFVSTGSAM
PQSMAHPCGGTPTYPES
QIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTV
GQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSD
YRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMK
QIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSIS
APISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGE
GGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPEL
TVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSD
VSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48466
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: WNK1 tv3 714-798 fused to HSN2 domain 13-425. Contains a silent mutation I285 in HSN2 and silent change in R3 of WNK1 tv3 insert.