cDNA Clone JAK2

• Plasmid: pSc-B-JAK2 (KO BAMHI)
• Parent Plasmid: pSC-B
• Genbank: NP_004963.1

Sequence of Insert

GGATCCATGGGAATGGCCTGCCTTACGATGACAGAAATGGAGGGAACATC
CACCTCTTCTATATATCAGAATGGTGATATTTCTGGAAATGCCAATTCTA
TGAAGCAAATAGATCCAGTTCTTCAGGTGTATCTTTACCATTCCCTTGGG
AAATCTGAGGCAGATTATCTGACCTTTCCATCTGGGGAGTATGTTGCAGA
AGAAATCTGTATTGCTGCTTCTAAAGCTTGTGGTATCACACCTGTGTATC
ATAATATGTTTGCTTTAATGAGTGAAACAGAAAGGATCTGGTATCCACCC
AACCATGTCTTCCATATAGATGAGTCAACCAGGCATAATGTACTCTACAG
AATAAGATTTTACTTTCCTCGTTGGTATTGCAGTGGCAGCAACAGAGCCT
ATCGGCATGGAATATCTCGAGGTGCTGAAGCTCCTCTTCTTGATGACTTT
GTCATGTCTTACCTCTTTGCTCAGTGGCGGCATGATTTTGTGCACGGATG
GATAAAAGTACCTGTGACTCATGAAACACAGGAAGAATGTCTTGGGATGG
CAGTGTTAGATATGATGAGAATAGCCAAAGAAAACGATCAAACCCCACTG
GCCATCTATAACTCTATCAGCTACAAGACATTCTTACCAAAATGTATTCG
AGCAAAGATCCAAGACTATCATATTTTGACAAGGAAGCGAATAAGGTACA
GATTTCGCAGATTTATTCAGCAATTCAGCCAATGCAAAGCCACTGCCAGA
AACTTGAAACTTAAGTATCTTATAAATCTGGAAACTCTGCAGTCTGCCTT
CTACACAGAGAAATTTGAAGTAAAAGAACCTGGAAGTGGTCCTTCAGGTG
AGGAGATTTTTGCAACCATTATAATAACTGGAAACGGTGGAATTCAGTGG
TCAAGAGGGAAACATAAAGAAAGTGAGACACTGACAGAACAGGATTTACA
GTTATATTGCGATTTTCCTAATATTATTGATGTCAGTATTAAGCAAGCAA
ACCAAGAGGGTTCAAATGAAAGCCGAGTTGTAACTATCCATAAGCAAGAT
GGTAAAAATCTGGAAATTGAACTTAGCTCATTAAGGGAAGCTTTGTCTTT
CGTGTCATTAATTGATGGATATTATAGATTAACTGCAGATGCACATCATT
ACCTCTGTAAAGAAGTAGCACCTCCAGCCGTGCTTGAAAATATACAAAGC
AACTGTCATGGCCCAATTTCGATGGATTTTGCCATTAGTAAACTGAAGAA
AGCAGGTAATCAGACTGGACTGTATGTACTTCGATGCAGTCCTAAGGACT
TTAATAAATATTTTTTGACTTTTGCTGTCGAGCGAGAAAATGTCATTGAA
TATAAACACTGTTTGATTACAAAAAATGAGAATGAAGAGTACAACCTCAG
TGGGACAAAGAAGAACTTCAGCAGTCTTAAAGATCTTTTGAATTGTTACC
AGATGGAAACTGTTCGCTCAGACAATATAATTTTCCAGTTTACTAAATGC
TGTCCCCCAAAGCCAAAAGATAAATCAAACCTTCTAGTCTTCAGAACGAA
TGGTGTTTCTGATGTACCAACCTCACCAACATTACAGAGGCCTACTCATA
TGAACCAAATGGTGTTTCACAAAATCAGAAATGAAGATTTGATATTTAAT
GAAAGCCTTGGCCAAGGCACTTTTACAAAGATTTTTAAAGGCGTACGAAG
AGAAGTAGGAGACTACGGTCAACTGCATGAAACAGAAGTTCTTTTAAAAG
TTCTGGATAAAGCACACAGAAACTATTCAGAGTCTTTCTTTGAAGCAGCA
AGTATGATGAGCAAGCTTTCTCACAAGCATTTGGTTTTAAATTATGGAGT
ATGTGTCTGTGGAGACGAGAATATTCTGGTTCAGGAGTTTGTAAAATTTG
GATCACTAGATACATATCTGAAAAAGAATAAAAATTGTATAAATATATTA
TGGAAACTTGAAGTTGCTAAACAGTTGGCATGGGCCATGCATTTTCTAGA
AGAAAACACCCTTATTCATGGGAATGTATGTGCCAAAAATATTCTGCTTA
TCAGAGAAGAAGACAGGAAGACAGGAAATCCTCCTTTCATCAAACTTAGT
GATCCTGGCATTAGTATTACAGTTTTGCCAAAGGACATTCTTCAGGAGAG
AATACCATGGGTACCACCTGAATGCATTGAAAATCCTAAAAATTTAAATT
TGGCAACAGACAAATGGAGTTTTGGTACCACTTTGTGGGAAATCTGCAGT
GGAGGAGATAAACCTCTAAGTGCTCTGGATTCTCAAAGAAAGCTACAATT
TTATGAAGATAGGCATCAGCTTCCTGCACCAAAGTGGGCAGAATTAGCAA
ACCTTATAAATAATTGTATGGATTATGAACCAGATTTCAGGCCTTCTTTC
AGAGCCATCATACGAGATCTTAACAGTTTGTTTACTCCAGATTATGAACT
ATTAACAGAAAATGACATGTTACCAAATATGAGGATAGGTGCCCTGGGGT
TTTCTGGTGCCTTTGAAGACCGGGACCCTACACAGTTTGAAGAGAGACAT
TTGAAATTTCTACAGCAACTTGGCAAGGGTAATTTTGGGAGTGTGGAGAT
GTGCCGGTATGACCCTCTACAGGACAACACTGGGGAGGTGGTCGCTGTAA
AAAAGCTTCAGCATAGTACTGAAGAGCACCTAAGAGACTTTGAAAGGGAA
ATTGAAATCCTGAAATCCCTACAGCATGACAACATTGTAAAGTACAAGGG
AGTGTGCTACAGTGCTGGTCGGCGTAATCTAAAATTAATTATGGAATATT
TACCATATGGAAGTTTACGAGACTATCTTCAAAAACATAAAGAACGGATA
GATCACATAAAACTTCTGCAGTACACATCTCAGATATGCAAGGGTATGGA
GTATCTTGGTACAAAAAGGTATATCCACAGGGATCTGGCAACGAGAAATA
TATTGGTGGAGAACGAGAACAGAGTTAAAATTGGAGATTTTGGGTTAACC
AAAGTCTTGCCACAAGACAAAGAATACTATAAAGTAAAAGAACCTGGTGA
AAGTCCCATATTCTGGTATGCTCCAGAATCACTGACAGAGAGCAAGTTTT
CTGTGGCCTCAGATGTTTGGAGCTTTGGAGTGGTTCTGTATGAACTTTTC
ACATACATTGAGAAGAGTAAAAGTCCACCAGCGGAATTTATGCGTATGAT
TGGCAATGACAAACAAGGACAGATGATCGTGTTCCATTTGATAGAACTTT
TGAAGAATAATGGAAGATTACCAAGACCAGATGGATGCCCAGATGAGATC
TATATGATCATGACAGAATGCTGGAACAATAATGTAAATCAACGCCCCTC
CTTTAGGGATCTAGCTCTTCGAGTGGATCAAATAAGGGATAACATGGCTG
GATGAGCGGCCGC

Amino Acid Sequence

MGMACLTMTEMEGTSTSSIYQNGDISGNANSMKQIDPVLQVYLYHSLGKS
EADYLTFPSGEYVAEEICIAASKACGITPVYHNMFALMSETERIWYPPNH
VFHIDESTRHNVLYRIRFYFPRWYCSGSNRAYRHGISRGAEAPLLDDFVM
SYLFAQWRHDFVHGWIKVPVTHETQEECLGMAVLDMMRIAKENDQTPLAI
YNSISYKTFLPKCIRAKIQDYHILTRKRIRYRFRRFIQQFSQCKATARNL
KLKYLINLETLQSAFYTEKFEVKEPGSGPSGEEIFATIIITGNGGIQWSR
GKHKESETLTEQDLQLYCDFPNIIDVSIKQANQEGSNESRVVTIHKQDGK
NLEIELSSLREALSFVSLIDGYYRLTADAHHYLCKEVAPPAVLENIQSNC
HGPISMDFAISKLKKAGNQTGLYVLRCSPKDFNKYFLTFAVERENVIEYK
HCLITKNENEEYNLSGTKKNFSSLKDLLNCYQMETVRSDNIIFQFTKCCP
PKPKDKSNLLVFRTNGVSDVPTSPTLQRPTHMNQMVFHKIRNEDLIFNES
LGQGTFTKIFKGVRREVGDYGQLHETEVLLKVLDKAHRNYSESFFEAASM
MSKLSHKHLVLNYGVCVCGDENILVQEFVKFGSLDTYLKKNKNCINILWK
LEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGNPPFIKLSDP
GISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGG
DKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRA
IIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLK
FLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIE
ILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDH
IKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKV
LPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTY
IEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYM
IMTECWNNNVNQRPSFRDLALRVDQIRDNMAG*

• Vector Type: Other
• DU Number: DU24643
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: internal BamHI at nt2526 KILLED