cDNA Clone C15ORF41

• Synonyms: HH114
• Plasmid: pcDNA5D FRT/TO C15orf41 TEV RFP 2A 2A GFP CDAN1
• Parent Plasmid: pcDNA5D FRT/TO mcs TEV RFP 2A 2A GFP mcs
• Expressed: C15orf41 TEV RFP 2A 2A GFP 3C CDAN1
• Genbank: NM_001130010.2 GI:589811559 (C15orf41) and NM_138477.2 GI:57222569 (CDAN1) with silent change g3660a and residue difference S421F (present in many published sequences for CDAN1)
• Tag: GFP
• Tag: mRFP
• Species: Human

Sequence of Insert

CACCGGTGGCATGGCGGCCGTTTTGGAGTCGCTGCTGCGAGAAGAGGTGTCGGTCGCAGCCGTCGTGCGGTGGATCGCGCGCAGCACCCAGGGTTCGGAGGATAACGCTGGGGAGGCGGCCGCGCTGAGCTCACTCCGGGCCCTGCGGAAAGAATTCGTACCGTTCCTGTTGAACTTCCTGAGGGAGCAGAGCAGCCGCGTCCTCCCGCAGGGGCCCCCGACCCCCGCCAAGACCCCGGGCGCCTCGGCAGCCTTGCCAGGGAGGCCGGGAGGCCCGCCGCGGGGTAGCCGCGGGGCGCGCAGCCAGCTTTTCCCTCCGACCGAGGCCCAGAGCACCGCTGCCGAGGCCCCTCTGGCCCGCCGCGGGGGCAGGAGGCGGGGCCCGGGGCCGGCCCGCGAGCGTGGAGGCCGCGGCCTGGAGGAGGGGGTCAGCGGGGAGAGCCTGCCCGGAGCCGGGGGCCGGAGGCTTAGGGGCTCTGGCAGCCCCAGCCGCCCCAGCCTCACGCTGTCTGATCCGCCAAACCTCAGCAACCTGGAGGAGTTCCCTCCCGTAGGCTCGGTTCCCCCCGGCCCTACAGGGACGAAGCCTTCTCGCAGGATCAACCCAACTCCGGTGAGCGAAGAGCGGTCACTCTCCAAGCCCAAGACCTGCTTCACCTCACCCCCAATCAGCTGTGTCCCCAGTTCCCAACCCTCAGCCCTGGACACTAGCCCTTGGGGCCTTGGCCTTCCCCCAGGGTGCAGAAGTCTGCAAGAGGAGCGAGAGATGCTCAGGAAGGAGCGCTCTAAGCAGCTGCAGCAGTCACCTACCCCCACCTGTCCCACCCCAGAATTGGGGTCGCCCCTCCCCAGCCGGACAGGAAGCCTCACAGATGAACCTGCAGACCCTGCCAGAGTGTCTTCCCGCCAGCGCCTGGAGCTTGTAGCCCTTGTTTACTCCTCGTGCATTGCTGAGAACCTGGTACCAAACCTCTTCTTGGAGCTTTTCTTCGTCTTTCAGCTCCTCACTGCCCGGAGGATGGTGACTGCCAAGGACAGCGACCCTGAACTAAGTCCAGCTGTCCTAGATTCCCTGGAAAGTCCACTGTTCCAAAGCATCCACGATTGTGTCTTCTTTGCAGTGCAGGTTTTGGAGTGTCACTTTCAGGTTCTTTCCAACCTGGACAAAGGGACCTTGAAGCTGCTGGCTGAGAATGAGCGGCTGCTGTGCTTCTCACCAGCTCTGCAAGGCCGCCTTCGAGCTGCCTATGAGGGCAGTGTTGCCAAGGTCTTTCTGGTGATGCCTCCCTCTACTCAAGCTGTCTCCTTTCAGCCAGAGACTGACAATCGTGCCAACTTCTCCAGTGACCGAGCCTTTCATACTTTTAAGAAACAGAGGGATGTGTTTTATGAGGTGCTGCGAGAGTGGGAAGATCACCATGAGGAGCCTGGCTGGGATTTTGAGAAGGGCTTGGGCAGCAGAATCAGGGCCATGATGGGTCAGCTCTCCGCAGCCTGCAGCCACAGCCACTTTGTTCGGCTTTTCCAAAAACAACTACTCCAGATGTGTCAGAGCCCTGGTGGTGCTGGGGGCACCGTCTTGGGCGAGGCCCCAGATGTGTTGAGTATGCTGGGAGCTGACAAGCTGGGGCGGTTGTGGCGCCTACAGGAACGGCTTATGGCTCCTCAGAGCAGTGGGGGGCCCTGCCCACCCCCCACCTTCCCAGGCTGTCAAGGCTTCTTTAGGGACTTCATCCTTAGTGCCAGCAGCTTCCAGTTTAACCAGCATCTCATGGACAGTCTGAGCTTGAAGATCCAGGAGCTCAATGGTCTTGCCCTGCCCCAGCATGAGCCCAATGATGAAGACGGGGAGTCAGACGTAGACTGGCAGGGTGAGCGGAAGCAATTTGCTGTGGTGCTTCTTAGCCTGAGACTTTTGGCTAAATTCCTGGGCTTTGTGGCTTTCCTGCCATACCGGGGGCCTGAACCTCCCCCGACCGGTGAGCTTCAGGACTCCATTCTGGCCCTCAGGAGCCAGGTCCCTCCGGTCCTGGATGTGCGGACTCTGCTGCAGCGAGGGCTGCAGGCCCGCCGGGCGGTGCTCACCGTGCCCTGGCTGGTGGAGTTTCTCTCCTTTGCTGACCATGTTGTTCCCTTGCTGGAATATTACCGGGACATCTTCACTCTCCTGCTGCGCCTGCACCGGAGCTTGGTGTTGTCGCAGGAGAGTGAGGGGAAGATGTGTTTCCTGAACAAGCTGCTGCTACTTGCTGTCCTGGGCTGGCTTTTCCAGATTCCCACAGTCCCTGAGGACTTGTTCTTTCTGGAAGAGGGTCCCTCATATGCCTTTGAGGTGGACACAGTAGCCCCAGAGCATGGCTTGGACAATGCGCCTGTGGTGGACCAGCAGCTGCTCTACACCTGCTGCCCCTACATCGGAGAGCTCCGGAAACTGCTCGCTTCGTGGGTGTCAGGCAGTAGTGGACGGAGTGGGGGCTTCATGAGGAAAATCACCCCCACCACTACCACCAGCCTGGGAGCCCAGCCTTCCCAGACCAGCCAGGGGCTGCAGGCACAGCTCGCCCAGGCCTTTTTCCACAACCAGCCGCCCTCCTTGCGCCGGACCGTAGAGTTCGTGGCAGAAAGAATTGGATCAAACTGTGTCAAACATATCAAGGCTACACTGGTGGCAGATCTGGTGCGCCAGGCAGAGTCACTTCTCCAAGAGCAGCTGGTGACACAGGGAGAGGAAGGGGGAGACCCAGCCCAGCTGTTGGAGATCTTGTGTTCCCAGCTGTGCCCTCACGGGGCCCAGGCATTGGCCCTGGGGCGGGAGTTCTGTCAAAGGAAGAGCCCTGGGGCTGTGCGGGCGCTGCTTCCAGAGGAGACCCCGGCAGCCGTTCTGAGCAGTGCAGAGAACATTGCTGTGGGGCTTGCAACAGAGAAAGCCTGTGCTTGGCTGTCAGCCAACATCACAGCACTGATCAGGAGGGAGGTGAAAGCAGCAGTGAGTCGCACACTTCGAGCCCAGGGTCCTGAACCTGCTGCCCGGGGGGAGCGGAGGGGCTGCTCCCGCGCCTGTGAGCACCATGCTCCCCTCCCCTCCCACCTCATCTCCGAGATAAAAGACGTGCTCTCCTTGGCCGTGGGGCCACGGGACCCTGACGAGGGAGTCTCCCCAGAGCATCTGGAACAGCTCCTAGGCCAGCTGGGCCAGACGCTGCGGTGCCGCCAGTTCCTGTGCCCACCTGCTGAGCAGCATCTGGCAAAGTGCTCTGTGGAGTTAGCTTCCCTCCTCGTTGCAGATCAAATTCCTATCCTAGGGCCCCCGGCACAGTACAGGCTGGAGAGAGGGCAGGCTCGAAGGCTTCTGCACATGCTGCTTTCCTTGTGGAAGGAAGACTTTCAGGGGCCGGTTCCGCTGCAGCTGCTGCTGAGCCCAAGAAATGTGGGGCTTCTGGCAGACACAAGGCCAAGGGAGTGGGACTTGCTGCTATTCTTGCTACGGGAGCTGGTGGAGAAGGGTCTGATGGGACGGATGGAGATAGAGGCCTGCCTGGGCAGCCTCCACCAGGCCCAGTGGCCAGGGGACTTTGCTGAAGAATTAGCAACACTGTCTAATCTGTTTCTAGCCGAGCCCCACCTGCCAGAACCCCAGCTAAGAGCCTGTGAGTTGGTGCAGCCAAACCGGGGCACTGTGCTGGCCCAGAGCTAGATGCAT

Amino Acid Sequence

MILTKAQYDEIAQCLVSVPPTRQSLRKLKQRFPSQSQATLLSIFSQEYQK
HIKRTHAKHHTSEAIESYYQRYLNGVVKNGAAPVLLDLANEVDYAPSLMA
RLILERFLQEHEETPPSKSIINSMLRDPSQIPDGVLANQVYQCIVNDCCY
GPLVDCIKHAIGHEHEVLLRDLLLEKNLSFLDEDQLRAKGYDKTPDFILQ
VPVAVEGHIIHWIESKASFGDECSHHAYLHDQFWSYWNRFGPGLVIYWYG
FIQELDCNRERGILLKACFPTNIVTLCHSIAAAAENLYFQMDNTEDVIKE
FMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAKLQVTKGGPLPFAWDI
LSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGVVEVQQD
SSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGE
ISHALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTV
VEQYEHAEARHSGSQEGRGSLLTCGDVEENPGPEGRGSLLTCGDVEENPG
PMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKLEVLFQGPTG
GMAAVLESLLREEVSVAAVVRWIARSTQGSEDNAGEAAALSSLRALRKEF
VPFLLNFLREQSSRVLPQGPPTPAKTPGASAALPGRPGGPPRGSRGARSQ
LFPPTEAQSTAAEAPLARRGGRRRGPGPARERGGRGLEEGVSGESLPGAG
GRRLRGSGSPSRPSLTLSDPPNLSNLEEFPPVGSVPPGPTGTKPSRRINP
TPVSEERSLSKPKTCFTSPPISCVPSSQPSALDTSPWGLGLPPGCRSLQE
EREMLRKERSKQLQQSPTPTCPTPELGSPLPSRTGSLTDEPADPARVSSR
QRLELVALVYSSCIAENLVPNLFLELFFVFQLLTARRMVTAKDSDPELSP
AVLDSLESPLFQSIHDCVFFAVQVLECHFQVLSNLDKGTLKLLAENERLL
CFSPALQGRLRAAYEGSVAKVFLVMPPSTQAVSFQPETDNRANFSSDRAF
HTFKKQRDVFYEVLREWEDHHEEPGWDFEKGLGSRIRAMMGQLSAACSHS
HFVRLFQKQLLQMCQSPGGAGGTVLGEAPDVLSMLGADKLGRLWRLQERL
MAPQSSGGPCPPPTFPGCQGFFRDFILSASSFQFNQHLMDSLSLKIQELN
GLALPQHEPNDEDGESDVDWQGERKQFAVVLLSLRLLAKFLGFVAFLPYR
GPEPPPTGELQDSILALRSQVPPVLDVRTLLQRGLQARRAVLTVPWLVEF
LSFADHVVPLLEYYRDIFTLLLRLHRSLVLSQESEGKMCFLNKLLLLAVL
GWLFQIPTVPEDLFFLEEGPSYAFEVDTVAPEHGLDNAPVVDQQLLYTCC
PYIGELRKLLASWVSGSSGRSGGFMRKITPTTTTSLGAQPSQTSQGLQAQ
LAQAFFHNQPPSLRRTVEFVAERIGSNCVKHIKATLVADLVRQAESLLQE
QLVTQGEEGGDPAQLLEILCSQLCPHGAQALALGREFCQRKSPGAVRALL
PEETPAAVLSSAENIAVGLATEKACAWLSANITALIRREVKAAVSRTLRA
QGPEPAARGERRGCSRACEHHAPLPSHLISEIKDVLSLAVGPRDPDEGVS
PEHLEQLLGQLGQTLRCRQFLCPPAEQHLAKCSVELASLLVADQIPILGP
PAQYRLERGQARRLLHMLLSLWKEDFQGPVPLQLLLSPRNVGLLADTRPR
EWDLLLFLLRELVEKGLMGRMEIEACLGSLHQAQWPGDFAEELATLSNLF
LAEPHLPEPQLRACELVQPNRGTVLAQS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50043
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CDAN1 from DU49995 cloned into DU50029 SgrA1/NsiI; Bicistronic expression plasmid. Single fusion protein expressed which should autocleave at 2A 2A sequence.