cDNA Clone C15orf41/CDAN1 (codanin)

• Synonyms: HH114, CDA1; CDAI; CDAN1A; DLT; PRO1295; codanin
• Plasmid: pcDNA5D FRT/TO C15orf41 3C FLAG 2A 2A 6His TEV CDAN1
• Parent Plasmid: pcDNA5D FRT/TO mcs 3C FLAG 2A 2A 6His TEV mcs
• Expressed: C15orf41 3C FLAG 2A 2A 6His TEV CDAN1
• Genbank: NM_138477.2 GI:57222569 with silent change g3660a and residue difference S421F (present in many published sequences for CDAN1) (CDAN1), NM_001130010.2 GI:589811559 (C15orf41)
• Tag: FLAG
• Tag: 6His
• Species: Human

Sequence of Insert

GGCGCGCCGATGGCGGCCGTTTTGGAGTCGCTGCTGCGAGAAGAGGTGTCGGTCGCAGCCGTCGTGCGGTGGATCGCGCGCAGCACCCAGGGTTCGGAGGATAACGCTGGGGAGGCGGCCGCGCTGAGCTCACTCCGGGCCCTGCGGAAAGAATTCGTACCGTTCCTGTTGAACTTCCTGAGGGAGCAGAGCAGCCGCGTCCTCCCGCAGGGGCCCCCGACCCCCGCCAAGACCCCGGGCGCCTCGGCAGCCTTGCCAGGGAGGCCGGGAGGCCCGCCGCGGGGTAGCCGCGGGGCGCGCAGCCAGCTTTTCCCTCCGACCGAGGCCCAGAGCACCGCTGCCGAGGCCCCTCTGGCCCGCCGCGGGGGCAGGAGGCGGGGCCCGGGGCCGGCCCGCGAGCGTGGAGGCCGCGGCCTGGAGGAGGGGGTCAGCGGGGAGAGCCTGCCCGGAGCCGGGGGCCGGAGGCTTAGGGGCTCTGGCAGCCCCAGCCGCCCCAGCCTCACGCTGTCTGATCCGCCAAACCTCAGCAACCTGGAGGAGTTCCCTCCCGTAGGCTCGGTTCCCCCCGGCCCTACAGGGACGAAGCCTTCTCGCAGGATCAACCCAACTCCGGTGAGCGAAGAGCGGTCACTCTCCAAGCCCAAGACCTGCTTCACCTCACCCCCAATCAGCTGTGTCCCCAGTTCCCAACCCTCAGCCCTGGACACTAGCCCTTGGGGCCTTGGCCTTCCCCCAGGGTGCAGAAGTCTGCAAGAGGAGCGAGAGATGCTCAGGAAGGAGCGCTCTAAGCAGCTGCAGCAGTCACCTACCCCCACCTGTCCCACCCCAGAATTGGGGTCGCCCCTCCCCAGCCGGACAGGAAGCCTCACAGATGAACCTGCAGACCCTGCCAGAGTGTCTTCCCGCCAGCGCCTGGAGCTTGTAGCCCTTGTTTACTCCTCGTGCATTGCTGAGAACCTGGTACCAAACCTCTTCTTGGAGCTTTTCTTCGTCTTTCAGCTCCTCACTGCCCGGAGGATGGTGACTGCCAAGGACAGCGACCCTGAACTAAGTCCAGCTGTCCTAGATTCCCTGGAAAGTCCACTGTTCCAAAGCATCCACGATTGTGTCTTCTTTGCAGTGCAGGTTTTGGAGTGTCACTTTCAGGTTCTTTCCAACCTGGACAAAGGGACCTTGAAGCTGCTGGCTGAGAATGAGCGGCTGCTGTGCTTCTCACCAGCTCTGCAAGGCCGCCTTCGAGCTGCCTATGAGGGCAGTGTTGCCAAGGTCTTTCTGGTGATGCCTCCCTCTACTCAAGCTGTCTCCTTTCAGCCAGAGACTGACAATCGTGCCAACTTCTCCAGTGACCGAGCCTTTCATACTTTTAAGAAACAGAGGGATGTGTTTTATGAGGTGCTGCGAGAGTGGGAAGATCACCATGAGGAGCCTGGCTGGGATTTTGAGAAGGGCTTGGGCAGCAGAATCAGGGCCATGATGGGTCAGCTCTCCGCAGCCTGCAGCCACAGCCACTTTGTTCGGCTTTTCCAAAAACAACTACTCCAGATGTGTCAGAGCCCTGGTGGTGCTGGGGGCACCGTCTTGGGCGAGGCCCCAGATGTGTTGAGTATGCTGGGAGCTGACAAGCTGGGGCGGTTGTGGCGCCTACAGGAACGGCTTATGGCTCCTCAGAGCAGTGGGGGGCCCTGCCCACCCCCCACCTTCCCAGGCTGTCAAGGCTTCTTTAGGGACTTCATCCTTAGTGCCAGCAGCTTCCAGTTTAACCAGCATCTCATGGACAGTCTGAGCTTGAAGATCCAGGAGCTCAATGGTCTTGCCCTGCCCCAGCATGAGCCCAATGATGAAGACGGGGAGTCAGACGTAGACTGGCAGGGTGAGCGGAAGCAATTTGCTGTGGTGCTTCTTAGCCTGAGACTTTTGGCTAAATTCCTGGGCTTTGTGGCTTTCCTGCCATACCGGGGGCCTGAACCTCCCCCGACCGGTGAGCTTCAGGACTCCATTCTGGCCCTCAGGAGCCAGGTCCCTCCGGTCCTGGATGTGCGGACTCTGCTGCAGCGAGGGCTGCAGGCCCGCCGGGCGGTGCTCACCGTGCCCTGGCTGGTGGAGTTTCTCTCCTTTGCTGACCATGTTGTTCCCTTGCTGGAATATTACCGGGACATCTTCACTCTCCTGCTGCGCCTGCACCGGAGCTTGGTGTTGTCGCAGGAGAGTGAGGGGAAGATGTGTTTCCTGAACAAGCTGCTGCTACTTGCTGTCCTGGGCTGGCTTTTCCAGATTCCCACAGTCCCTGAGGACTTGTTCTTTCTGGAAGAGGGTCCCTCATATGCCTTTGAGGTGGACACAGTAGCCCCAGAGCATGGCTTGGACAATGCGCCTGTGGTGGACCAGCAGCTGCTCTACACCTGCTGCCCCTACATCGGAGAGCTCCGGAAACTGCTCGCTTCGTGGGTGTCAGGCAGTAGTGGACGGAGTGGGGGCTTCATGAGGAAAATCACCCCCACCACTACCACCAGCCTGGGAGCCCAGCCTTCCCAGACCAGCCAGGGGCTGCAGGCACAGCTCGCCCAGGCCTTTTTCCACAACCAGCCGCCCTCCTTGCGCCGGACCGTAGAGTTCGTGGCAGAAAGAATTGGATCAAACTGTGTCAAACATATCAAGGCTACACTGGTGGCAGATCTGGTGCGCCAGGCAGAGTCACTTCTCCAAGAGCAGCTGGTGACACAGGGAGAGGAAGGGGGAGACCCAGCCCAGCTGTTGGAGATCTTGTGTTCCCAGCTGTGCCCTCACGGGGCCCAGGCATTGGCCCTGGGGCGGGAGTTCTGTCAAAGGAAGAGCCCTGGGGCTGTGCGGGCGCTGCTTCCAGAGGAGACCCCGGCAGCCGTTCTGAGCAGTGCAGAGAACATTGCTGTGGGGCTTGCAACAGAGAAAGCCTGTGCTTGGCTGTCAGCCAACATCACAGCACTGATCAGGAGGGAGGTGAAAGCAGCAGTGAGTCGCACACTTCGAGCCCAGGGTCCTGAACCTGCTGCCCGGGGGGAGCGGAGGGGCTGCTCCCGCGCCTGTGAGCACCATGCTCCCCTCCCCTCCCACCTCATCTCCGAGATAAAAGACGTGCTCTCCTTGGCCGTGGGGCCACGGGACCCTGACGAGGGAGTCTCCCCAGAGCATCTGGAACAGCTCCTAGGCCAGCTGGGCCAGACGCTGCGGTGCCGCCAGTTCCTGTGCCCACCTGCTGAGCAGCATCTGGCAAAGTGCTCTGTGGAGTTAGCTTCCCTCCTCGTTGCAGATCAAATTCCTATCCTAGGGCCCCCGGCACAGTACAGGCTGGAGAGAGGGCAGGCTCGAAGGCTTCTGCACATGCTGCTTTCCTTGTGGAAGGAAGACTTTCAGGGGCCGGTTCCGCTGCAGCTGCTGCTGAGCCCAAGAAATGTGGGGCTTCTGGCAGACACAAGGCCAAGGGAGTGGGACTTGCTGCTATTCTTGCTACGGGAGCTGGTGGAGAAGGGTCTGATGGGACGGATGGAGATAGAGGCCTGCCTGGGCAGCCTCCACCAGGCCCAGTGGCCAGGGGACTTTGCTGAAGAATTAGCAACACTGTCTAATCTGTTTCTAGCCGAGCCCCACCTGCCAGAACCCCAGCTAAGAGCCTGTGAGTTGGTGCAGCCAAACCGGGGCACTGTGCTGGCCCAGAGCTAGATGCAT

Amino Acid Sequence

MILTKAQYDEIAQCLVSVPPTRQSLRKLKQRFPSQSQATLLSIFSQEYQK
HIKRTHAKHHTSEAIESYYQRYLNGVVKNGAAPVLLDLANEVDYAPSLMA
RLILERFLQEHEETPPSKSIINSMLRDPSQIPDGVLANQVYQCIVNDCCY
GPLVDCIKHAIGHEHEVLLRDLLLEKNLSFLDEDQLRAKGYDKTPDFILQ
VPVAVEGHIIHWIESKASFGDECSHHAYLHDQFWSYWNRFGPGLVIYWYG
FIQELDCNRERGILLKACFPTNIVTLCHSIAAAALEVLFQGPDYKDDDDK
EGRGSLLTCGDVEENPGPEGRGSLLTCGDVEENPGPHHHHHHENLYFQTG
GAPMAAVLESLLREEVSVAAVVRWIARSTQGSEDNAGEAAALSSLRALRK
EFVPFLLNFLREQSSRVLPQGPPTPAKTPGASAALPGRPGGPPRGSRGAR
SQLFPPTEAQSTAAEAPLARRGGRRRGPGPARERGGRGLEEGVSGESLPG
AGGRRLRGSGSPSRPSLTLSDPPNLSNLEEFPPVGSVPPGPTGTKPSRRI
NPTPVSEERSLSKPKTCFTSPPISCVPSSQPSALDTSPWGLGLPPGCRSL
QEEREMLRKERSKQLQQSPTPTCPTPELGSPLPSRTGSLTDEPADPARVS
SRQRLELVALVYSSCIAENLVPNLFLELFFVFQLLTARRMVTAKDSDPEL
SPAVLDSLESPLFQSIHDCVFFAVQVLECHFQVLSNLDKGTLKLLAENER
LLCFSPALQGRLRAAYEGSVAKVFLVMPPSTQAVSFQPETDNRANFSSDR
AFHTFKKQRDVFYEVLREWEDHHEEPGWDFEKGLGSRIRAMMGQLSAACS
HSHFVRLFQKQLLQMCQSPGGAGGTVLGEAPDVLSMLGADKLGRLWRLQE
RLMAPQSSGGPCPPPTFPGCQGFFRDFILSASSFQFNQHLMDSLSLKIQE
LNGLALPQHEPNDEDGESDVDWQGERKQFAVVLLSLRLLAKFLGFVAFLP
YRGPEPPPTGELQDSILALRSQVPPVLDVRTLLQRGLQARRAVLTVPWLV
EFLSFADHVVPLLEYYRDIFTLLLRLHRSLVLSQESEGKMCFLNKLLLLA
VLGWLFQIPTVPEDLFFLEEGPSYAFEVDTVAPEHGLDNAPVVDQQLLYT
CCPYIGELRKLLASWVSGSSGRSGGFMRKITPTTTTSLGAQPSQTSQGLQ
AQLAQAFFHNQPPSLRRTVEFVAERIGSNCVKHIKATLVADLVRQAESLL
QEQLVTQGEEGGDPAQLLEILCSQLCPHGAQALALGREFCQRKSPGAVRA
LLPEETPAAVLSSAENIAVGLATEKACAWLSANITALIRREVKAAVSRTL
RAQGPEPAARGERRGCSRACEHHAPLPSHLISEIKDVLSLAVGPRDPDEG
VSPEHLEQLLGQLGQTLRCRQFLCPPAEQHLAKCSVELASLLVADQIPIL
GPPAQYRLERGQARRLLHMLLSLWKEDFQGPVPLQLLLSPRNVGLLADTR
PREWDLLLFLLRELVEKGLMGRMEIEACLGSLHQAQWPGDFAEELATLSN
LFLAEPHLPEPQLRACELVQPNRGTVLAQS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50080
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bicistronic plasmid which should autocleave at 2A 2A sequence.