cDNA Clone DNA2 (YEAST)

• Plasmid: pRS413 DNA2 D120-S1522(end) D212A D213A L215A I218A (YEAST)
• Parent Plasmid: pRS413
• Expressed: DNA2 D120-S1522(end) D212A D213A L215A I218A (YEAST)
• Genbank: NP_012034.1
• Species: Yeast

Sequence of Insert

GAGCTCGACGGCCCCATGGAAGAAGTAATATGGAAATATTCACCTTTACAACGAGATATGTCCGACAAAACAACAAGCGCTGCCGAATACTCTGATGACTACGAAGATGTCCAAAATCCCTCTTCTACACCTATAGTCCCTAATCGACTGAAAACTGTTCTTAGTTTCACAAATATCCAAGTACCCAACGCTGACGTAAATCAACTCATTCAAGAAAATGGAAATGAGCAAGTGCGTCCTAAACCAGCAGAAATATCTACCAGGGAGTCTCTCCGTAATATAGCTGCTATTGCCGATGATGCAGAGGGCGATTTAACTATAAAACCGACGATAACGAAATTCAGCGATTTGCCATCTTCACCCATCAAGGCACCCAACGTTGAAAAAAAAGCAGAGGTGAATGCAGAAGAAGTAGATAAAATGGATTCAACAGGAGATAGCAACGATGGCGATGACTCTTTGATAGATATTTTAACTCAAAAGTATGTTGAGAAACGCAAGAGTGAGAGTCAGATAACAATTCAAGGCAACACCAATCAAAAAAGTGGAGCCCAGGAAAGTTGTGGGAAGAATGATAACACAAAATCGAGAGGAGAAATTGAAGATCATGAAAATGTAGACAATCAAGCCAAAACAGGCAATGCATTTTATGAGAATGAAGAAGACAGTAATTGCCAAAGAATTAAAAAAAATGAAAAAATAGAATACAATTCTTCAGACGAATTTTCAGACGATTCACTAATTGAACTATTGAACGAAACTCAAACACAAGTGGAACCTAACACTATTGAACAAGATTTAGACAAAGTGGAGAAAATGGTCTCTGACGATTTGAGAATTGCTACTGATTCCACTTTATCCGCATATGCTCTCAGAGCTAAGTCTGGTGCTCCAAGAGACGGCGTAGTACGATTAGTAATAGTAAGCTTACGTAGTGTTGAATTGCCAAAAATTGGAACGCAAAAAATACTAGAGTGTATAGACGGCAAAGGCGAACAAAGTTCTGTTGTCGTTCGACATCCTTGGGTATATTTGGAATTTGAGGTTGGGGATGTAATACATATTATAGAAGGGAAAAATATTGAAAACAAACGGCTTCTTTCTGACGACAAAAACCCTAAAACTCAACTGGCTAACGACAACCTGTTGGTGCTAAACCCTGATGTATTATTTTCGGCAACATCAGTCGGAAGTTCAGTAGGTTGTTTAAGACGTTCAATTCTGCAAATGCAATTTCAAGATCCCCGCGGAGAACCAAGCCTTGTCATGACTTTAGGCAATATCGTACACGAGTTATTGCAAGACTCAATCAAATACAAGCTCTCGCATAATAAAATTTCTATGGAAATCATAATACAAAAGCTGGATTCTTTGTTAGAAACTTATTCTTTTTCAATTATCATTTGTAATGAAGAAATCCAGTACGTTAAAGAATTAGTTATGAAAGAACATGCTGAAAACATTTTGTACTTCGTCAATAAATTTGTTTCGAAAAGTAACTATGGTTGCTACACATCAATTTCCGGAACAAGAAGAACACAGCCAATTTCTATATCCAATGTGATTGATATTGAAGAAAACATTTGGTCTCCAATATATGGTCTTAAAGGGTTCCTAGATGCAACTGTTGAAGCTAATGTTGAAAACAATAAAAAACACATCGTTCCTTTAGAAGTGAAAACTGGAAAATCCAGAAGCGTTTCATACGAGGTTCAAGGCCTTATTTATACACTTCTGTTAAATGATCGCTATGAGATACCGATAGAGTTTTTTCTTCTTTATTTTACGAGGGATAAAAATATGACAAAGTTCCCTTCTGTTCTGCACTCCATTAAACATATTCTCATGTCCCGAAATAGAATGAGTATGAATTTTAAGCATCAACTCCAAGAGGTTTTCGGACAGGCTCAATCAAGGTTTGAATTACCACCCTTACTGCGCGATTCATCTTGTGATTCATGTTTCATCAAAGAATCATGCATGGTGTTGAATAAGCTACTAGAGGACGGGACACCAGAAGAAAGTGGCCTGGTAGAAGGTGAGTTTGAAATTTTGACAAACCATTTGTCGCAAAATCTCGCCAACTACAAAGAGTTTTTTACAAAATATAATGATCTAATAACTAAGGAGGAGTCAAGTATAACATGTGTAAATAAAGAACTTTTCTTATTGGATGGTTCCACAAGAGAATCCAGAAGTGGAAGGTGCCTTTCTGGCTTGGTTGTGTCAGAGGTGGTTGAACATGAAAAAACAGAGGGAGCGTATATATATTGCTTCTCAAGACGTAGAAATGATAATAACTCGCAGTCGATGTTATCATCTCAAATTGCTGCTAATGACTTTGTTATTATTAGTGATGAGGAGGGTCATTTTTGCTTGTGCCAAGGTCGTGTACAATTTATAAATCCCGCTAAAATTGGTATCTCCGTAAAACGAAAGTTATTAAACAATAGGCTTCTAGATAAGGAAAAAGGAGTCACTACCATCCAAAGTGTGGTCGAATCAGAGCTAGAACAATCCAGTCTCATAGCTACCCAAAATTTGGTCACATATAGAATCGACAAAAATGATATCCAACAGAGTTTGTCTTTAGCGCGATTTAATCTTTTAAGCTTGTTTTTACCAGCTGTTTCACCTGGCGTGGATATTGTTGACGAAAGGAGTAAATTATGTCGGAAGACAAAGAGATCTGATGGAGGTAATGAAATACTGCGATCACTTTTAGTTGATAATAGAGCACCTAAGTTTAGAGATGCAAATGATGATCCCGTCATTCCGTATAAGCTTTCAAAGGATACAACATTGAACTTGAATCAAAAAGAAGCAATAGACAAAGTAATGAGGGCCGAAGATTATGCATTGATATTGGGAATGCCAGGGACTGGTAAGACTACTGTTATCGCAGAAATTATTAAAATTCTCGTTTCCGAGGGGAAACGCGTGCTTTTGACATCATACACACATTCAGCAGTGGATAATATTTTAATAAAATTGAGGAACACCAACATTAGCATAATGAGATTGGGAATGAAACATAAGGTCCATCCTGACACGCAAAAATATGTCCCGAATTACGCGTCAGTGAAAAGTTATAACGATTATTTGTCCAAAATTAACAGCACCTCTGTTGTTGCTACCACCTGCTTAGGAATTAACGATATACTATTTACACTAAATGAAAAGGATTTTGATTATGTGATTTTGGACGAGGCAAGTCAAATTTCAATGCCTGTCGCTTTGGGACCTCTGCGGTATGGAAATAGGTTTATTATGGTAGGAGATCATTACCAGCTACCGCCATTAGTTAAAAATGACGCAGCTCGATTAGGTGGGTTAGAAGAGTCACTGTTCAAGACTTTCTGTGAAAAGCATCCGGAAAGCGTTGCAGAACTGACTTTACAGTACCGTATGTGCGGCGATATTGTAACTTTGTCTAATTTCCTAATTTATGATAATAAATTAAAATGCGGGAATAACGAAGTTTTTGCTCAATCTTTAGAGTTACCAATGCCTGAGGCCTTGTCGCGCTATAGGAATGAATCGGCTAACTCTAAGCAGTGGCTTGAGGATATACTAGAGCCTACAAGAAAAGTGGTATTTTTAAATTACGATAACTGCCCGGATATTATCGAACAAAGCGAAAAGGATAACATTACGAATCACGGGGAGGCAGAATTGACTCTACAATGTGTGGAAGGAATGTTATTGAGCGGCGTACCATGCGAAGATATTGGTGTAATGACGTTATACAGAGCACAGTTAAGATTACTGAAGAAGATATTCAACAAAAATGTGTATGACGGGCTAGAGATCTTGACTGCTGATCAGTTTCAAGGTCGTGACAAAAAGTGCATTATTATTTCCATGGTTAGAAGAAATTCTCAATTAAATGGAGGAGCGCTGCTGAAAGAGCTAAGAAGAGTCAATGTTGCCATGACAAGGGCCAAATCCAAGCTGATCATTATAGGTTCAAAAAGTACAATCGGCTCTGTTCCTGAAATAAAATCGTTCGTGAACCTGTTAGAAGAGCGTAATTGGGTCTATACAATGTGCAAGGATGCACTCTACAAATACAAATTTCCCGACAGATCAAACGCTATTGATGAGGCACGCAAAGGTTGTGGAAAAAGAACAGGTGCCAAACCCATCACATCAAAGTCTAAATTCGTAAGCGATAAACCTATCATAAAGGAAATTCTACAAGAGTATGAAAGTGAATTC

Amino Acid Sequence

ELDGPMEEVIWKYSPLQRDMSDKTTSAAEYSDDYEDVQNPSSTPIVPNRLKTVLSFTNIQVPNADVNQLIQENGNEQVRPKPAEISTRESLRNIAAIADDAEGDLTIKPTITKFSDLPSSPIKAPNVEKKAEVNAEEVDKMDSTGDSNDGDDSLIDILTQKYVEKRKSESQITIQGNTNQKSGAQESCGKNDNTKSRGEIEDHENVDNQAKTGNAFYENEEDSNCQRIKKNEKIEYNSSDEFSDDSLIELLNETQTQVEPNTIEQDLDKVEKMVSDDLRIATDSTLSAYALRAKSGAPRDGVVRLVIVSLRSVELPKIGTQKILECIDGKGEQSSVVVRHPWVYLEFEVGDVIHIIEGKNIENKRLLSDDKNPKTQLANDNLLVLNPDVLFSATSVGSSVGCLRRSILQMQFQDPRGEPSLVMTLGNIVHELLQDSIKYKLSHNKISMEIIIQKLDSLLETYSFSIIICNEEIQYVKELVMKEHAENILYFVNKFVSKSNYGCYTSISGTRRTQPISISNVIDIEENIWSPIYGLKGFLDATVEANVENNKKHIVPLEVKTGKSRSVSYEVQGLIYTLLLNDRYEIPIEFFLLYFTRDKNMTKFPSVLHSIKHILMSRNRMSMNFKHQLQEVFGQAQSRFELPPLLRDSSCDSCFIKESCMVLNKLLEDGTPEESGLVEGEFEILTNHLSQNLANYKEFFTKYNDLITKEESSITCVNKELFLLDGSTRESRSGRCLSGLVVSEVVEHEKTEGAYIYCFSRRRNDNNSQSMLSSQIAANDFVIISDEEGHFCLCQGRVQFINPAKIGISVKRKLLNNRLLDKEKGVTTIQSVVESELEQSSLIATQNLVTYRIDKNDIQQSLSLARFNLLSLFLPAVSPGVDIVDERSKLCRKTKRSDGGNEILRSLLVDNRAPKFRDANDDPVIPYKLSKDTTLNLNQKEAIDKVMRAEDYALILGMPGTGKTTVIAEIIKILVSEGKRVLLTSYTHSAVDNILIKLRNTNISIMRLGMKHKVHPDTQKYVPNYASVKSYNDYLSKINSTSVVATTCLGINDILFTLNEKDFDYVILDEASQISMPVALGPLRYGNRFIMVGDHYQLPPLVKNDAARLGGLEESLFKTFCEKHPESVAELTLQYRMCGDIVTLSNFLIYDNKLKCGNNEVFAQSLELPMPEALSRYRNESANSKQWLEDILEPTRKVVFLNYDNCPDIIEQSEKDNITNHGEAELTLQCVEGMLLSGVPCEDIGVMTLYRAQLRLLKKIFNKNVYDGLEILTADQFQGRDKKCIIISMVRRNSQLNGGALLKELRRVNVAMTRAKSKLIIIGSKSTIGSVPEIKSFVNLLEERNWVYTMCKDALYKYKFPDRSNAIDEARKGCGKRTGAKPITSKSKFVSDKPIIKEILQEYES

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU25948
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Restriction site in between aa456 and 457 deleted