cDNA Clone NEK9

• Synonyms: NERCC, NERCC1, Nek8
• Plasmid: pSCB NEK9 Nhe Not
• Parent Plasmid: pSC-B
• Genbank: NM_033116.4

Sequence of Insert

GCTAGCATGTCGGTGCTGGGCGAGTACGAGCGACACTGCGATTCCATCAACTCGGACTTTGGGAGCGAGTCCGGGGGTTGCGGGGACTCGAGTCCGGGGCCTAGCGCCAGTCAGGGGCCGCGAGCCGGCGGCGGCGCGGCGGAGCAGGAGGAACTGCACTACATCCCCATCCGCGTCCTGGGCCGCGGCGCCTTCGGGGAAGCCACGCTGTACCGCCGCACCGAGGATGACTCACTGGTTGTGTGGAAGGAAGTCGATTTGACCCGGCTGTCTGAGAAGGAACGTCGTGATGCCTTGAATGAGATAGTTATTCTGGCACTGCTGCAGCACGACAACATTATTGCCTACTACAATCACTTCATGGACAATACCACGCTGCTGATTGAGCTGGAATATTGTAATGGAGGGAACCTGTATGACAAAATCCTTCGTCAGAAGGACAAGTTGTTTGAGGAAGAGATGGTGGTGTGGTACCTATTTCAGATTGTTTCAGCAGTGAGCTGCATCCATAAAGCTGGAATCCTTCATAGAGATATAAAGACATTAAATATTTTTCTGACCAAGGCAAACCTGATAAAACTTGGAGATTATGGCCTAGCAAAGAAACTTAATTCTGAGTATTCCATGGCTGAGACGCTTGTGGGAACCCCATATTACATGTCTCCAGAGCTCTGTCAAGGAGTAAAGTACAATTTCAAGTCTGATATCTGGGCAGTTGGCTGCGTCATTTTTGAACTGCTTACCTTAAAGAGGACGTTTGATGCTACAAACCCACTTAACCTGTGTGTGAAGATCGTGCAAGGAATTCGGGCCATGGAAGTTGACTCTAGCCAGTACTCTTTGGAATTGATCCAAATGGTTCATTCGTGCCTTGACCAGGATCCTGAGCAGAGACCTACTGCAGATGAACTTCTAGATCGCCCTCTTCTCAGGAAACGCAGGAGAGAGATGGAGGAAAAAGTCACTCTGCTTAATGCACCTACAAAGAGACCAAGGTCAAGCACTGTGACTGAAGCACCCATTGCTGTAGTAACATCACGAACCAGTGAAGTCTATGTTTGGGGTGGTGGAAAATCCACCCCCCAGAAACTGGATGTTATCAAGAGTGGCTGTAGTGCCCGGCAGGTCTGTGCAGGGAATACCCACTTTGCTGTGGTCACAGTGGAGAAGGAACTGTACACTTGGGTGAACATGCAAGGAGGCACTAAACTCCATGGTCAGCTGGGCCATGGAGACAAAGCCTCCTATCGACAGCCAAAGCATGTGGAAAAGTTGCAAGGCAAAGCTATCCGTCAGGTGTCATGTGGTGATGATTTCACTGTCTGTGTGACTGATGAGGGTCAGCTCTATGCCTTCGGATCAGATTATTATGGCTGCATGGGGGTGGACAAAGTTGCTGGCCCTGAAGTGCTAGAACCCATGCAGCTGAACTTCTTCCTCAGCAATCCAGTGGAGCAGGTCTCCTGTGGAGATAATCATGTGGTGGTTCTGACACGAAACAAGGAAGTCTATTCTTGGGGCTGTGGCGAATATGGACGACTGGGTTTGGATTCAGAAGAGGATTATTATACACCACAAAAGGTGGATGTTCCCAAGGCCTTGATTATTGTTGCAGTTCAATGTGGCTGTGATGGGACATTTCTGTTGACCCAGTCAGGCAAAGTGCTGGCCTGTGGACTCAATGAATTCAATAAGCTGGGTCTGAATCAGTGCATGTCGGGAATTATCAACCATGAAGCATACCATGAAGTTCCCTACACAACGTCCTTTACCTTGGCCAAACAGTTGTCCTTTTATAAGATCCGTACCATTGCCCCAGGCAAGACTCACACAGCTGCTATTGATGAGCGAGGCCGGCTGCTGACCTTTGGCTGCAACAAGTGTGGGCAGCTGGGCGTTGGGAACTACAAGAAGCGTCTGGGAATCAACCTGTTGGGGGGACCCCTTGGTGGGAAGCAAGTGATCAGGGTCTCCTGCGGTGATGAGTTTACCATTGCTGCCACTGATGATAATCACATTTTTGCCTGGGGCAATGGTGGTAATGGCCGCCTGGCAATGACCCCCACAGAGAGACCACATGGCTCTGATATCTGTACCTCATGGCCTCGGCCTATTTTTGGATCTCTGCATCATGTCCCGGACCTGTCTTGCCGTGGATGGCATACCATTCTCATCGTTGAGAAAGTATTGAATTCTAAGACCATCCGTTCCAATAGCAGTGGCTTATCCATTGGAACTGTGTTTCAGAGCTCTAGCCCGGGAGGAGGCGGCGGGGGCGGCGGTGGTGAAGAAGAGGACAGTCAGCAGGAATCTGAAACTCCTGACCCAAGTGGAGGCTTCCGAGGAACAATGGAAGCAGACCGAGGAATGGAAGGTTTAATCAGTCCCACAGAGGCCATGGGGAACAGTAATGGGGCCAGCAGCTCCTGTCCTGGCTGGCTTCGAAAGGAGCTGGAAAATGCAGAATTTATCCCCATGCCTGACAGCCCATCTCCTCTCAGTGCAGCGTTTTCAGAATCTGAGAAAGATACCCTGCCCTATGAAGAGCTGCAAGGACTCAAAGTGGCCTCTGAAGCTCCTTTGGAACACAAACCCCAAGTAGAAGCCTCGTCACCTCGGCTGAATCCTGCAGTAACCTGTGCTGGGAAGGGAACACCACTGACTCCTCCTGCGTGTGCGTGCAGCTCTCTGCAGGTGGAGGTTGAGAGATTGCAGGGTCTGGTGTTAAAGTGTCTGGCTGAACAACAGAAGCTACAGCAAGAAAACCTCCAGATTTTTACCCAACTGCAGAAGTTGAACAAGAAATTAGAAGGAGGGCAGCAGGTGGGGATGCATTCCAAAGGAACTCAGACAGCAAAGGAAGAGATGGAAATGGATCCAAAGCCTGACTTAGATTCAGATTCCTGGTGCCTCCTGGGAACAGACTCCTGTAGACCCAGCCTCTAGCGGCCGC

Amino Acid Sequence

MSVLGEYERHCDSINSDFGSESGGCGDSSPGPSASQGPRAGGGAAEQEEL
HYIPIRVLGRGAFGEATLYRRTEDDSLVVWKEVDLTRLSEKERRDALNEI
VILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKILRQKDKLFEE
EMVVWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKK
LNSEYSMAETLVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRT
FDATNPLNLCVKIVQGIRAMEVDSSQYSLELIQMVHSCLDQDPEQRPTAD
ELLDRPLLRKRRREMEEKVTLLNAPTKRPRSSTVTEAPIAVVTSRTSEVY
VWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGT
KLHGQLGHGDKASYRQPKHVEKLQGKAIRQVSCGDDFTVCVTDEGQLYAF
GSDYYGCMGVDKVAGPEVLEPMQLNFFLSNPVEQVSCGDNHVVVLTRNKE
VYSWGCGEYGRLGLDSEEDYYTPQKVDVPKALIIVAVQCGCDGTFLLTQS
GKVLACGLNEFNKLGLNQCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIR
TIAPGKTHTAAIDERGRLLTFGCNKCGQLGVGNYKKRLGINLLGGPLGGK
QVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERPHGSDICTSWP
RPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGLSIGTVFQSSS
PGGGGGGGGGEEEDSQQESETPDPSGGFRGTMEADRGMEGLISPTEAMGN
SNGASSSCPGWLRKELENAEFIPMPDSPSPLSAAFSESEKDTLPYEELQG
LKVASEAPLEHKPQVEASSPRLNPAVTCAGKGTPLTPPACACSSLQVEVE
RLQGLVLKCLAEQQKLQQENLQIFTQLQKLNKKLEGGQQVGMHSKGTQTA
KEEMEMDPKPDLDSDSWCLLGTDSCRPSL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48614
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent T>A nucleotide change at position 300 bp.