cDNA Clone CLASP1

• Synonyms: MAST1
• Plasmid: pCMV5 FLAG2 CLASP1 tv3
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG-CLASP1 tv3
• Genbank: NM_001142274.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCAATGGAGCCTCGCATGGAGTCCTGCCTGGCGCAGGTGTTGCAGAAGGATGTGGGGAAACGATTGCAGGTTGGCCAAGAACTGATAGACTATTTCTCAGACAAACAGAAGTCTGCTGACCTTGAGCATGACCAGACCATGTTAGATAAACTTGTGGATGGACTTGCTACCTCTTGGGTGAACTCTAGCAATTACAAGGTGGTTCTGCTGGGCATGGACATCCTGTCCGCCCTGGTGACCCGGCTGCAGGATCGGTTCAAGGCGCAGATCGGCACAGTGCTGCCAAGTCTAATAGACAGACTAGGAGATGCTAAAGACTCTGTGAGGGAGCAGGACCAAACTCTGCTGCTAAAGATCATGGATCAAGCTGCTAATCCCCAGTACGTATGGGACAGAATGCTTGGAGGCTTCAAACACAAGAATTTCCGTACTCGAGAAGGCATCTGTCTCTGCCTTATAGCAACACTCAATGCCTCTGGAGCACAGACTTTAACACTAAGCAAGATTGTGCCACATATATGCAACTTACTTGGAGATCCAAACAGCCAGGTTCGAGATGCAGCAATAAACAGCTTAGTGGAAATTTACAGACATGTAGGAGAACGTGTGAGGGCAGATCTCAGTAAAAAAGGATTGCCACAGTCCCGGTTGAATGTAATTTTTACAAAATTTGATGAAGTCCAGAAATCTGGAAACATGATACAATCTGCAAATGATAAAAATTTCGACGATGAAGATTCTGTGGATGGTAACAGACCTTCCTCTGCTAGTTCTACATCATCCAAGGCTCCACCAAGTTCTCGGAGAAACGTTGGAATGGGAACCACCCGCCGGCTTGGTTCATCCACCCTTGGATCCAAGTCTTCAGCTGCAAAAGAAGGAGCTGGTGCTGTTGATGAAGAGGATTTTATTAAAGCATTTGATGATGTACCTGTAGTACAGATTTATTCCAGCCGAGACCTTGAGGAATCCATAAACAAAATTAGGGAAATATTATCTGATGACAAGCATGATTGGGAGCAGAGAGTAAATGCTCTAAAAAAGATTAGATCTTTACTTTTGGCTGGTGCTGCTGAGTATGATAACTTCTTTCAACATTTGCGTCTTTTGGATGGAGCCTTTAAACTCTCTGCTAAGGACCTGCGGTCTCAGGTAGTGCGGGAGGCTTGTATCACGTTGGGGCATCTGTCATCAGTTCTGGGGAATAAGTTTGACCATGGAGCTGAAGCCATTATGCCAACTATCTTTAATTTAATTCCAAACAGTGCCAAAATTATGGCCACATCTGGTGTTGTAGCTGTTAGGTTAATTATTCGGCACACACACATCCCTAGGTTAATACCTGTCATAACAAGCAACTGTACCTCTAAGTCTGTCGCAGTTAGAAGGCGCTGTTTTGAATTTTTAGATTTGCTTTTACAAGAATGGCAGACACATTCACTAGAACGACACATATCAGTATTAGCTGAAACAATAAAGAAGGGAATACATGATGCTGATTCCGAAGCAAGAATAGAAGCCAGAAAATGTTACTGGGGTTTCCACAGTCACTTCAGCAGAGAAGCAGAGCACTTGTACCACACCTTGGAGTCCTCCTACCAGAAAGCCCTGCAGTCCCACCTGAAGAACTCAGACAGCATAGTGTCTCTGCCTCAGTCAGACCGCTCATCTTCCAGCTCTCAAGAGAGTCTAAATCGTCCGCTGTCTGCCAAAAGAAGTCCTACTGGAAGTACCACATCTAGAGCTTCTACAGTTAGTACCAAATCTGTGTCAACGACTGGGTCCCTCCAGCGATCTCGAAGTGATATTGATGTGAACGCAGCAGCCAGTGCCAAATCCAAAGTCTCCTCATCTTCGGGCACGACGCCTTTCAGCTCTGCAGCAGCTTTGCCTCCAGGGTCATACGCATCCTTAGGTCGGATCCGCACAAGACGGCAAAGCTCTGGGAGTGCCACCAACGTCGCCTCTACACCTGATAACCGGGGCCGCAGTCGCGCTAAAGTGGTTTCACAGTCCCAGCGATCCAGATCTGCTAATCCTGCTGGTGCTGGCAGCCGGTCAAGTTCCCCAGGAAAATTGTTGGGAAGTGGTTATGGTGGACTTACTGGGGGCTCCTCACGAGGCCCACCTGTGACACCGTCTTCAGAAAAGCGAAGCAAGATTCCCAGGAGCCAGGGATGTAGCCGGGAAACAAGTCCAAACCGAATAGGATTAGATCGGTTTGGGCTTGGCCAGCCAGGAAGAATACCTGGTTCTGTGAATGCCATGAGAGTTCTGAGCACAAGTACAGATCTTGAAGCTGCTGTTGCTGATGCTTTGCTGTTAGGAGACTCCAGGAGCAAGAAGAAGCCTGTGAGGAGGAGATATGAGCCGTATGGGATGTATTCTGACGATGATGCCAACAGTGATGCCTCAAGTGTTTGCTCTGAGCGCTCATATGGCTCCAGGAATGGTGGCATTCCCCATTATCTGCGGCAGACTGAGGATGTAGCAGAAGTTCTCAACCACTGTGCTAGTTCAAACTGGTCAGAAAGGAAAGAAGGGCTTCTGGGCCTGCAGAACTTACTGAAGAGCCAAAGAACACTGAGTCGAGTTGAACTGAAAAGGTTGTGTGAGATCTTCACTCGGATGTTTGCTGACCCTCATAGCAAGAGAGTTTTCAGTATGTTTTTGGAGACTCTTGTGGATTTTATAATAATTCATAAGGATGATTTACAAGACTGGCTTTTTGTTCTTCTCACACAATTACTTAAGAAAATGGGAGCAGATTTACTTGGATCTGTGCAAGCAAAAGTTCAAAAGGCTCTAGATGTCACAAGGGACTCCTTTCCATTTGATCAACAATTTAACATTTTGATGAGATTTATTGTGGATCAAACTCAAACTCCAAACCTCAAGGTCAAAGTTGCAATCCTGAAATACATTGAGTCTCTGGCCAGACAGATGGATCCAACAGATTTTGTAAACTCTAGTGAGACAAGGCTTGCTGTTTCTAGAATCATAACCTGGACAACAGAACCAAAGAGTTCAGACGTGAGAAAGGCAGCACAGATTGTGCTAATCTCTCTGTTTGAATTGAATACTCCTGAATTTACCATGTTACTTGGTGCCTTGCCAAAAACATTCCAGGATGGTGCCACCAAACTCCTGCACAACCACCTCAAGAATTCCAGTAACACCAGTGTGGGCTCTCCAAGCAATACGATTGGCCGGACGCCCTCCCGACACACCAGCAGCAGGACCAGCCCCCTGACCTCACCCACCAACTGTTCCCATGGGGGTCTGTCTCCAAGCATGCTGGACTATGATACAGAGAACCTGAACTCTGAAGAAATCTATAGTTCTCTACGTGGAGTTACAGAAGCCATTGAAAAGTTTAGTTTTCGAAGCCAAGAAGATCTGAATGAGCCAATTAAACGAGATGGCAAAAAGGAGTGTGATATTGTGTCCCGCGATGGGGGCGCTGCCTCCCCTGCCACTGAGGGCCGGGGGGGTAGTGAAGTAGAAGGAGGCCGGACAGCTCTGGATAACAAGACCTCACTACTCAACACCCAGCCTCCGCGCGCCTTCCCGGGGCCGCGGGCGCGAGACTACAACCCGTACCCCTACTCAGATGCCATCAACACCTACGACAAGACCGCCCTGAAAGAGGCTGTGTTCGATGACGACATGGAGCAGCTTCGAGACGTGCCCATCGACCATTCTGACCTGGTGGCTGACCTTCTGAAAGAGCTGTCCAACCACAATGAGCGAGTGGAGGAACGGAAGGGAGCCCTGCTGGAGCTGCTCAAGATCACGCGGGAAGACAGCCTTGGTGTCTGGGAGGAGCACTTCAAGACCATTCTGCTCCTGCTGCTGGAGACCCTTGGAGACAAAGACCATTCAATTCGAGCACTGGCGTTAAGAGTTTTGAGGGAAATTCTGAGAAATCAACCAGCAAGATTTAAAAACTACGCCGAGCTGACGATTATGAAGACTCTGGAAGCCCACAAAGACTCCCATAAGGAGGTGGTGAGAGCGGCTGAGGAGGCTGCGTCCACACTGGCCAGTTCCATCCACCCGGAGCAGTGCATCAAGGTGCTCTGCCCCATCATCCAGACGGCCGACTACCCCATCAACCTTGCTGCCATCAAGATGCAGACCAAAGTCGTCGAGAGGATCGCAAAGGAGTCATTGCTGCAGCTCCTTGTCGACATCATCCCAGGCTTGCTGCAGGGTTATGACAACACCGAAAGTAGTGTGCGTAAGGCCAGCGTGTTTTGCTTAGTGGCAATTTATTCCGTAATCGGAGAAGACCTGAAACCTCACCTTGCACAGCTCACAGGGAGCAAGATGAAGCTACTAAACTTATACATAAAGAGGGCCCAGACCACCAACAGCAACAGCAGCTCCTCCTCCGATGTCTCCACGCACAGCTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMEPRMESCLAQVLQKDVGKRLQVGQELI
DYFSDKQKSADLEHDQTMLDKLVDGLATSWVNSSNYKVVLLGMDILSALV
TRLQDRFKAQIGTVLPSLIDRLGDAKDSVREQDQTLLLKIMDQAANPQYV
WDRMLGGFKHKNFRTREGICLCLIATLNASGAQTLTLSKIVPHICNLLGD
PNSQVRDAAINSLVEIYRHVGERVRADLSKKGLPQSRLNVIFTKFDEVQK
SGNMIQSANDKNFDDEDSVDGNRPSSASSTSSKAPPSSRRNVGMGTTRRL
GSSTLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIR
EILSDDKHDWEQRVNALKKIRSLLLAGAAEYDNFFQHLRLLDGAFKLSAK
DLRSQVVREACITLGHLSSVLGNKFDHGAEAIMPTIFNLIPNSAKIMATS
GVVAVRLIIRHTHIPRLIPVITSNCTSKSVAVRRRCFEFLDLLLQEWQTH
SLERHISVLAETIKKGIHDADSEARIEARKCYWGFHSHFSREAEHLYHTL
ESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQESLNRPLSAKRSPTGSTT
SRASTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGTTPFSSAAA
LPPGSYASLGRIRTRRQSSGSATNVASTPDNRGRSRAKVVSQSQRSRSAN
PAGAGSRSSSPGKLLGSGYGGLTGGSSRGPPVTPSSEKRSKIPRSQGCSR
ETSPNRIGLDRFGLGQPGRIPGSVNAMRVLSTSTDLEAAVADALLLGDSR
SKKKPVRRRYEPYGMYSDDDANSDASSVCSERSYGSRNGGIPHYLRQTED
VAEVLNHCASSNWSERKEGLLGLQNLLKSQRTLSRVELKRLCEIFTRMFA
DPHSKRVFSMFLETLVDFIIIHKDDLQDWLFVLLTQLLKKMGADLLGSVQ
AKVQKALDVTRDSFPFDQQFNILMRFIVDQTQTPNLKVKVAILKYIESLA
RQMDPTDFVNSSETRLAVSRIITWTTEPKSSDVRKAAQIVLISLFELNTP
EFTMLLGALPKTFQDGATKLLHNHLKNSSNTSVGSPSNTIGRTPSRHTSS
RTSPLTSPTNCSHGGLSPSMLDYDTENLNSEEIYSSLRGVTEAIEKFSFR
SQEDLNEPIKRDGKKECDIVSRDGGAASPATEGRGGSEVEGGRTALDNKT
SLLNTQPPRAFPGPRARDYNPYPYSDAINTYDKTALKEAVFDDDMEQLRD
VPIDHSDLVADLLKELSNHNERVEERKGALLELLKITREDSLGVWEEHFK
TILLLLLETLGDKDHSIRALALRVLREILRNQPARFKNYAELTIMKTLEA
HKDSHKEVVRAAEEAASTLASSIHPEQCIKVLCPIIQTADYPINLAAIKM
QTKVVERIAKESLLQLLVDIIPGLLQGYDNTESSVRKASVFCLVAIYSVI
GEDLKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSSSDVSTHS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48819
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: variant 3