cDNA Clone DNA2 (yeast)

• Plasmid: pGADT7 5GA DNA2 (yeast) Full Length
• Parent Plasmid: pGADT7
• Expressed: HA 5GA DNA2 Yeast
• Genbank: NP_012034.1
• Species: Yeast

Sequence of Insert

GAATTCGGAGCTGGTGCAGGCGCTGGAGCGGGTGCCATGCCCGGAACGCCACAGAAGAACAAGAGGTCTGCGAGTATATCTGTTTCACCTGCGAAGAAGACAGAGGAAAAAGAAATAATACAAAATGATTCAAAGGCGATATTATCTAAGCAAACTAAGAGGAAAAAAAAGTATGCTTTTGCTCCTATAAATAACTTAAATGGGAAGAACACCAAGGTATCCAATGCAAGTGTCCTAAAGTCAATAGCCGTTTCACAAGTACGAAATACATCAAGAACAAAAGATATAAACAAAGCAGTTAGCAAGAGCGTAAAGCAATTACCAAATTCACAAGTTAAACCGAAACGGGAAATGTCTAATTTGAGTAGGCATCACGATTTCACTCAAGACGAGGACGGCCCCATGGAAGAAGTAATATGGAAATATTCACCTTTACAACGAGATATGTCCGACAAAACAACAAGCGCTGCCGAATACTCTGATGACTACGAAGATGTCCAAAATCCCTCTTCTACACCTATAGTCCCTAATCGACTGAAAACTGTTCTTAGTTTCACAAATATCCAAGTACCCAACGCTGACGTAAATCAACTCATTCAAGAAAATGGAAATGAGCAAGTGCGTCCTAAACCAGCAGAAATATCTACCAGGGAGTCTCTCCGTAATATAGATGATATTCTCGATGATATAGAGGGCGATTTAACTATAAAACCGACGATAACGAAATTCAGCGATTTGCCATCTTCACCCATCAAGGCACCCAACGTTGAAAAAAAAGCAGAGGTGAATGCAGAAGAAGTAGATAAAATGGATTCAACAGGAGATAGCAACGATGGCGATGACTCTTTGATAGATATTTTAACTCAAAAGTATGTTGAGAAACGCAAGAGTGAGAGTCAGATAACAATTCAAGGCAACACCAATCAAAAAAGTGGAGCCCAGGAAAGTTGTGGGAAGAATGATAACACAAAATCGAGAGGAGAAATTGAAGATCATGAAAATGTAGACAATCAAGCCAAAACAGGCAATGCATTTTATGAGAATGAAGAAGACAGTAATTGCCAAAGAATTAAAAAAAATGAAAAAATAGAATACAATTCTTCAGACGAATTTTCAGACGATTCACTAATTGAACTATTGAACGAAACTCAAACACAAGTGGAACCTAACACTATTGAACAAGATTTAGACAAAGTGGAGAAAATGGTCTCTGACGATTTGAGAATTGCTACTGATTCCACTTTATCCGCATATGCTCTCAGAGCTAAGTCTGGTGCTCCAAGAGACGGCGTAGTACGATTAGTAATAGTAAGCTTACGTAGTGTTGAATTGCCAAAAATTGGAACGCAAAAAATACTAGAGTGTATAGACGGCAAAGGCGAACAAAGTTCTGTTGTCGTTCGACATCCTTGGGTATATTTGGAATTTGAGGTTGGGGATGTAATACATATTATAGAAGGGAAAAATATTGAAAACAAACGGCTTCTTTCTGACGACAAAAACCCTAAAACTCAACTGGCTAACGACAACCTGTTGGTGCTAAACCCTGATGTATTATTTTCGGCAACATCAGTCGGAAGTTCAGTAGGTTGTTTAAGACGTTCAATTCTGCAAATGCAATTTCAAGATCCCCGCGGAGAACCAAGCCTTGTCATGACTTTAGGCAATATCGTACACGAGTTATTGCAAGACTCAATCAAATACAAGCTCTCGCATAATAAAATTTCTATGGAAATCATAATACAAAAGCTGGATTCTTTGTTAGAAACTTATTCTTTTTCAATTATCATTTGTAATGAAGAAATCCAGTACGTTAAAGAATTAGTTATGAAAGAACATGCTGAAAACATTTTGTACTTCGTCAATAAATTTGTTTCGAAAAGTAACTATGGTTGCTACACATCAATTTCCGGAACAAGAAGAACACAGCCAATTTCTATATCCAATGTGATTGATATTGAAGAAAACATTTGGTCTCCAATATATGGTCTTAAAGGGTTCCTAGATGCAACTGTTGAAGCTAATGTTGAAAACAATAAAAAACACATCGTTCCTTTAGAAGTGAAAACTGGAAAATCCAGAAGCGTTTCATACGAGGTTCAAGGCCTTATTTATACACTTCTGTTAAATGATCGCTATGAGATACCGATAGAGTTTTTTCTTCTTTATTTTACGAGGGATAAAAATATGACAAAGTTCCCTTCTGTTCTGCACTCCATTAAACATATTCTCATGTCCCGAAATAGAATGAGTATGAATTTTAAGCATCAACTCCAAGAGGTTTTCGGACAGGCTCAATCAAGGTTTGAATTACCACCCTTACTGCGCGATTCATCTTGTGATTCATGTTTCATCAAAGAATCATGCATGGTGTTGAATAAGCTACTAGAGGACGGGACACCAGAAGAAAGTGGCCTGGTAGAAGGTGAGTTTGAAATTTTGACAAACCATTTGTCGCAAAATCTCGCCAACTACAAAGAGTTTTTTACAAAATATAATGATCTAATAACTAAGGAGGAGTCAAGTATAACATGTGTAAATAAAGAACTTTTCTTATTGGATGGTTCCACAAGAGAATCCAGAAGTGGAAGGTGCCTTTCTGGCTTGGTTGTGTCAGAGGTGGTTGAACATGAAAAAACAGAGGGAGCGTATATATATTGCTTCTCAAGACGTAGAAATGATAATAACTCGCAGTCGATGTTATCATCTCAAATTGCTGCTAATGACTTTGTTATTATTAGTGATGAGGAGGGTCATTTTTGCTTGTGCCAAGGTCGTGTACAATTTATAAATCCCGCTAAAATTGGTATCTCCGTAAAACGAAAGTTATTAAACAATAGGCTTCTAGATAAGGAAAAAGGAGTCACTACCATCCAAAGTGTGGTCGAATCAGAGCTAGAACAATCCAGTCTCATAGCTACCCAAAATTTGGTCACATATAGAATCGACAAAAATGATATCCAACAGAGTTTGTCTTTAGCGCGATTTAATCTTTTAAGCTTGTTTTTACCAGCTGTTTCACCTGGCGTGGATATTGTTGACGAAAGGAGTAAATTATGTCGGAAGACAAAGAGATCTGATGGAGGTAATGAAATACTGCGATCACTTTTAGTTGATAATAGAGCACCTAAGTTTAGAGATGCAAATGATGATCCCGTCATTCCGTATAAGCTTTCAAAGGATACAACATTGAACTTGAATCAAAAAGAAGCAATAGACAAAGTAATGAGGGCCGAAGATTATGCATTGATATTGGGAATGCCAGGGACTGGTAAGACTACTGTTATCGCAGAAATTATTAAAATTCTCGTTTCCGAGGGGAAACGCGTGCTTTTGACATCATACACACATTCAGCAGTGGATAATATTTTAATAAAATTGAGGAACACCAACATTAGCATAATGAGATTGGGAATGAAACATAAGGTCCATCCTGACACGCAAAAATATGTCCCGAATTACGCGTCAGTGAAAAGTTATAACGATTATTTGTCCAAAATTAACAGCACCTCTGTTGTTGCTACCACCTGCTTAGGAATTAACGATATACTATTTACACTAAATGAAAAGGATTTTGATTATGTGATTTTGGACGAGGCAAGTCAAATTTCAATGCCTGTCGCTTTGGGACCTCTGCGGTATGGAAATAGGTTTATTATGGTAGGAGATCATTACCAGCTACCGCCATTAGTTAAAAATGACGCAGCTCGATTAGGTGGGTTAGAAGAGTCACTGTTCAAGACTTTCTGTGAAAAGCATCCGGAAAGCGTTGCAGAACTGACTTTACAGTACCGTATGTGCGGCGATATTGTAACTTTGTCTAATTTCCTAATTTATGATAATAAATTAAAATGCGGGAATAACGAAGTTTTTGCTCAATCTTTAGAGTTACCAATGCCTGAGGCCTTGTCGCGCTATAGGAATGAATCGGCTAACTCTAAGCAGTGGCTTGAGGATATACTAGAGCCTACAAGAAAAGTGGTATTTTTAAATTACGATAACTGCCCGGATATTATCGAACAAAGCGAAAAGGATAACATTACGAATCACGGGGAGGCAGAATTGACTCTACAATGTGTGGAAGGAATGTTATTGAGCGGCGTACCATGCGAAGATATTGGTGTAATGACGTTATACAGAGCACAGTTAAGATTACTGAAGAAGATATTCAACAAAAATGTGTATGACGGGCTAGAGATCTTGACTGCTGATCAGTTTCAAGGTCGTGACAAAAAGTGCATTATTATTTCCATGGTTAGAAGAAATTCTCAATTAAATGGAGGAGCGCTGCTGAAAGAGCTAAGAAGAGTCAATGTTGCCATGACAAGGGCCAAATCCAAGCTGATCATTATAGGTTCAAAAAGTACAATCGGCTCTGTTCCTGAAATAAAATCGTTCGTGAACCTGTTAGAAGAGCGTAATTGGGTCTATACAATGTGCAAGGATGCACTCTACAAATACAAATTTCCCGACAGATCAAACGCTATTGATGAGGCACGCAAAGGTTGTGGAAAAAGAACAGGTGCCAAACCCATCACATCAAAGTCTAAATTCGTAAGCGATAAACCTATCATAAAGGAAATTCTACAAGAGTATGAAAGTTGAGGATCC

Amino Acid Sequence

MEYPYDVPDYAHMAMEASEFGAGAGAGAGAMPGTPQKNKRSASISVSPAKKTEEKEIIQNDSKAILSKQTKRKKKYAFAPINNLNGKNTKVSNASVLKSIAVSQVRNTSRTKDINKAVSKSVKQLPNSQVKPKREMSNLSRHHDFTQDEDGPMEEVIWKYSPLQRDMSDKTTSAAEYSDDYEDVQNPSSTPIVPNRLKTVLSFTNIQVPNADVNQLIQENGNEQVRPKPAEISTRESLRNIDDILDDIEGDLTIKPTITKFSDLPSSPIKAPNVEKKAEVNAEEVDKMDSTGDSNDGDDSLIDILTQKYVEKRKSESQITIQGNTNQKSGAQESCGKNDNTKSRGEIEDHENVDNQAKTGNAFYENEEDSNCQRIKKNEKIEYNSSDEFSDDSLIELLNETQTQVEPNTIEQDLDKVEKMVSDDLRIATDSTLSAYALRAKSGAPRDGVVRLVIVSLRSVELPKIGTQKILECIDGKGEQSSVVVRHPWVYLEFEVGDVIHIIEGKNIENKRLLSDDKNPKTQLANDNLLVLNPDVLFSATSVGSSVGCLRRSILQMQFQDPRGEPSLVMTLGNIVHELLQDSIKYKLSHNKISMEIIIQKLDSLLETYSFSIIICNEEIQYVKELVMKEHAENILYFVNKFVSKSNYGCYTSISGTRRTQPISISNVIDIEENIWSPIYGLKGFLDATVEANVENNKKHIVPLEVKTGKSRSVSYEVQGLIYTLLLNDRYEIPIEFFLLYFTRDKNMTKFPSVLHSIKHILMSRNRMSMNFKHQLQEVFGQAQSRFELPPLLRDSSCDSCFIKESCMVLNKLLEDGTPEESGLVEGEFEILTNHLSQNLANYKEFFTKYNDLITKEESSITCVNKELFLLDGSTRESRSGRCLSGLVVSEVVEHEKTEGAYIYCFSRRRNDNNSQSMLSSQIAANDFVIISDEEGHFCLCQGRVQFINPAKIGISVKRKLLNNRLLDKEKGVTTIQSVVESELEQSSLIATQNLVTYRIDKNDIQQSLSLARFNLLSLFLPAVSPGVDIVDERSKLCRKTKRSDGGNEILRSLLVDNRAPKFRDANDDPVIPYKLSKDTTLNLNQKEAIDKVMRAEDYALILGMPGTGKTTVIAEIIKILVSEGKRVLLTSYTHSAVDNILIKLRNTNISIMRLGMKHKVHPDTQKYVPNYASVKSYNDYLSKINSTSVVATTCLGINDILFTLNEKDFDYVILDEASQISMPVALGPLRYGNRFIMVGDHYQLPPLVKNDAARLGGLEESLFKTFCEKHPESVAELTLQYRMCGDIVTLSNFLIYDNKLKCGNNEVFAQSLELPMPEALSRYRNESANSKQWLEDILEPTRKVVFLNYDNCPDIIEQSEKDNITNHGEAELTLQCVEGMLLSGVPCEDIGVMTLYRAQLRLLKKIFNKNVYDGLEILTADQFQGRDKKCIIISMVRRNSQLNGGALLKELRRVNVAMTRAKSKLIIIGSKSTIGSVPEIKSFVNLLEERNWVYTMCKDALYKYKFPDRSNAIDEARKGCGKRTGAKPITSKSKFVSDKPIIKEILQEYES*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU25966
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee