cDNA Clone IRGQ

• Plasmid: pBabeD puro flag IRGQ siRNA resistant W421A L424A
• Parent Plasmid: pBabeD puro FLAG
• Expressed: Flag IRGQ siRNA resistant W421A L424A
• Genbank: NM_001007561.2 GI:209364510
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCTATGCCTCCGCCGCAGGGTGACGTGACCGCCTTGTTCCTGGGGCCTCCGGGCTTGGGGAAGTCCGCGCTGATCGCAGCGCTGTGCGACAAGGATGTGGAGACGCTCGAGGCCCCCGAGGGACGGCCGGACTCCGGGGTTCCCAGCCTAAGAGCTGCGGGCCCAGGCCTTTTTCTGGGCGAGCTGAGCTGCCCACCCGCAGCGCCGGGGCCCTGGGCGGCGGAAGCCAACGTGCTGGTACTGGTGCTGCCCGGACCCGAGGGGAACGGGGAACCGTTGGCTCCAGCCCTGGGAGAGGCAGCGCTGGCCGCCCTGGCCCGAGGGACCCCGCTACTGGCTGTGCGGAACCTCCGTCCTGGGGATTCACAGACTGCCGCCCAGGCCCGTGATCAGACAGCAGCTCTGCTGAACAGCGCGGGGTTAGGAGCTGCGGATCTGTTTGTGCTACCGGCGAACTGCGGCAGCAGCGACGGCTGCGAGGAGCTAGAGCGCCTCCGGGCGGCGCTGCAGAGCCAGGCAGAAGCGCTGCGGAGGCTCCTGCCACCGGCGCAGGATGGCTTCGAGGTGTTGGGTGCAGCAGAGCTAGAGGCTGTGCGTGAGGCCTTTGAGACCGGCGGCCTTGAGGCTGCGCTGTCGTGGGTGCGCTCAGGCCTGGAGCGCCTGGGCAGCGCACGGCTAGACCTGGCCGTGGCTGGCAAGGCTGACGTGGGCCTTGTGGTGGACATGCTGCTTGGATTGGATCCTGGCGACCCAGGCGCTGCGCCTGCTTCGGTCCCCACAGCACCCACTCCCTTCCCAGCCCCAGAGCGCCCGAATGTGGTGCTCTGGACCGTGCCTCTGGGCCACACGGGCACTGCCACCACCGCGGCCGCCGCCTCTCACCCAACGCACTACGACGCCCTCATCCTCGTCACCCCTGGGGCCCCCACTGAGAAGGACTGGGCCCAGGTCCAGGCCTTGCTGCTACCAGATGCGCCTCTTGTCTGCGTGCGCACAGACGGCGAGGGCGAGGATCCGGAGTGTCTGGGAGAAGGCAAAATGGAAAACCCTAAGGGCGAGAGCTTAAAGAACGCAGGTGGAGGGGGATTGGAGAATGCACTCAGTAAGGGAAGGGAGAAATGTAGCGCTGGATCGCAGAAAGCAGGCAGCGGGGAAGGTCCTGGGAAAGCTGGCAGCGAGGGTTTGCAGCAGGTTGTCGGCATGAAGAAATCAGGTGGTGGCGACTCAGAGCGGGCCGCTGCGTTAAGCCCGGAGGACGAGACGGCGGAGGTGGCNGANGAGGCGCCGCCGCCAGTGTTCCCCCTACGGCCTGGCGGACTCCCAGGGCTATGCGAATGGCTGCGGCGAGCGCTCCCCCCAGCCCAGGCAGGGGCACTGCTGCTGGCGTTGCCACCAGCATCTCCCAGCGCTGCCCGAACCAAGGCTGCGGCGTTGCGAGCCGGGGCGTGGAGGCCAGCTCTGCTGGCTAGTCTGGCGGCGGCGGCGGCACCACTCCCAGGGCTGGGCTGGGCATGCGACGTGGCACTTCTGCGGGGTCAGCTGGCGGAGTGGCGACGGGGCCTGGGGCTGGAACCCACGGCACTGGCTCGACGTGAGCGTGCCCTGGGCCTGGCTTCTGGAGAGCTGGCAGCGCGCGCTCATTTCCCAGGCCCGGTGACGCGCGCCGAGGTGGAAGCAAGACTGGGCGCCTGGGCGGGCGAGGGCACTGCTGGGGGCGCAGCACTGGGGGCTCTCTCCTTCCTGTGGCCTGCGGGTGGTGCAGCGGCGACAGGTGGCCTGGGCTACCGAGCGGCTCACGGCGTCCTGCTGCAGGCTCTCGATGAGATGCGGGCTGATGCTGAGGCTGTGCTGGCACCCCCTGAGCCTGCCCAGTGAGATCC

Amino Acid Sequence

MDYKDDDDKGSMPPPQGDVTALFLGPPGLGKSALIAALCDKDVETLEAPEGRPDSGVPSLRAAGPGLFLGELSCPPAAPGPWAAEANVLVLVLPGPEGNGEPLAPALGEAALAALARGTPLLAVRNLRPGDSQTAAQARDQTAALLNSAGLGAADLFVLPANCGSSDGCEELERLRAALQSQAEALRRLLPPAQDGFEVLGAAELEAVREAFETGGLEAALSWVRSGLERLGSARLDLAVAGKADVGLVVDMLLGLDPGDPGAAPASVPTAPTPFPAPERPNVVLWTVPLGHTGTATTAAAASHPTHYDALILVTPGAPTEKDWAQVQALLLPDAPLVCVRTDGEGEDPECLGEGKMENPKGESLKNAGGGGLENALSKGREKCSAGSQKAGSGEGPGKAGSEGLQQVVGMKKSGGGDSERAAALSPEDETAEVXXEAPPPVFPLRPGGLPGLCEWLRRALPPAQAGALLLALPPASPSAARTKAAALRAGAWRPALLASLAAAAAPLPGLGWACDVALLRGQLAEWRRGLGLEPTALARRERALGLASGELAARAHFPGPVTRAEVEARLGAWAGEGTAGGAALGALSFLWPAGGAAATGGLGYRAAHGVLLQALDEMRADAEAVLAPPEPAQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU26086
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: nucleotide sequence AAG ATG GAG AAT CCC mutated to AAA ATG GAA AAC CCT to produce an siRNA resistant mutant.