cDNA Clone CYLD

• Plasmid: pcDNA5D FRT/TO BirA R118G-CYLD C598A-HA (codon optimised seq)
• Parent Plasmid: pcDNA5D FRT/TO BirA R118G MCS HA
• Expressed: BirA R118G-CYLD C598A-HA
• Genbank: Q9NQC7
• Tag: HA
• Tag: BirA
• Species: Human

Sequence of Insert

GGATCCATGAGTTCAGGCTTATGGAGCCAAGAAAAAGTCACTTCACCCTA
CTGGGAAGAGCGGATTTTTTACTTGCTTCTTCAAGAATGCAGCGTTACAG
ACAAACAAACACAAAAGCTCCTTAAAGTACCGAAGGGAAGTATAGGACAG
TATATTCAAGATCGTTCTGTGGGGCATTCAAGGATTCCTTCTGCAAAAGG
CAAGAAAAATCAGATTGGATTAAAAATTCTAGAGCAACCTCATGCAGTTC
TCTTTGTTGATGAAAAGGATGTTGTAGAGATAAATGAAAAGTTCACAGAG
TTACTTTTGGCAATTACCAATTGTGAGGAGAGGTTCAGCCTGTTTAAAAA
CAGAAACAGACTAAGTAAAGGCCTCCAAATAGACGTGGGCTGTCCTGTGA
AAGTACAGCTGAGATCTGGGGAAGAAAAATTTCCTGGAGTTGTACGCTTC
AGAGGACCCCTGTTAGCAGAGAGGACAGTCTCCGGAATATTCTTTGGAGT
TGAATTGCTGGAAGAAGGTCGTGGTCAAGGTTTCACTGACGGGGTGTACC
AAGGGAAACAGCTTTTTCAGTGTGATGAAGATTGTGGCGTGTTTGTTGCA
TTGGACAAGCTAGAACTCATAGAAGATGATGACACTGCATTGGAAAGTGA
TTACGCAGGTCCTGGGGACACAATGCAGGTCGAACTTCCTCCTTTGGAAA
TAAACTCCAGAGTTTCTTTGAAGGTTGGAGAAACAATAGAATCTGGAACA
GTTATATTCTGTGATGTTTTGCCAGGAAAAGAAAGCTTAGGATATTTTGT
TGGTGTGGACATGGATAACCCTATTGGCAACTGGGATGGAAGATTTGATG
GAGTGCAGCTTTGTAGTTTTGCGTGTGTTGAAAGTACAATTCTATTGCAC
ATCAATGATATCATCCCAGagagTGTGACGCAGGAAAGGAGGCCTCCCAA
ACTTGCCTTTATGTCAAGAGGTGTTGGGGACAAAGGTTCATCCAGTCATA
ATAAACCAAAGGCTACAGGATCTACCTCAGACCCTGGAAATAGAAACAGA
TCTGAATTATTTTATACCTTAAATGGGTCTTCTGTTGACTCACAACCACA
ATCCAAATCAAAAAATACATGGTACATTGATGAAGTTGCAGAAGACCCTG
CAAAATCTCTTACAGAGATATCTACAGACTTTGACCGTTCTTCACCACCA
CTCCAGCCTCCTCCTGTGAACTCACTGACCACCGAGAACAGATTCCACTC
TTTACCATTCAGTCTCACCAAGATGCCCAATACCAATGGAAGTATTGGCC
ACAGTCCACTTTCTCTGTCAGCCCAGTCTGTAATGGAAGAGCTAAACACT
GCACCCGTCCAAGAGAGTCCACCCTTGGCCATGCCTCCTGGGAACTCACA
TGGTCTAGAAGTGGGCTCATTGGCTGAAGTTAAGGAGAACCCTCCTTTCT
ATGGGGTAATCCGTTGGATCGGTCAGCCACCAGGACTGAATGAAGTGCTC
GCTGGACTGGAACTGGAAGATGAGTGTGCAGGCTGTACGGATGGAACCTT
CAGAGGCACTCGGTATTTCACCTGTGCCCTGAAGAAGGCGCTGTTTGTGA
AACTGAAGAGCTGCAGGCCTGACTCTAGGTTTGCATCATTGCAGCCGGTT
TCCAATCAGATTGAGCGCTGTAACTCTTTAGCATTTGGAGGCTACTTAAG
TGAAGTAGTAGAAGAAAATACTCCACCAAAAATGGAAAAAGAAGGCTTGG
AGATAATGATTGGGAAGAAGAAAGGCATCCAGGGTCATTACAATTCTgcT
TACTTAGACTCAACCTTATTCTGCTTATTTGCTTTTAGTTCTGTTCTGGA
CACTGTGTTACTTAGACCCAAAGAAAAGAACGATGTAGAATATTATAGTG
AAACCCAAGAGCTACTGAGGACAGAAATTGTTAATCCTCTGAGAATATAT
GGATATGTGTGTGCCACAAAAATTATGAAACTGAGGAAAATACTTGAAAA
GGTGGAGGCTGCATCAGGATTTACCTCTGAAGAAAAAGATCCTGAGGAAT
TCTTGAATATTCTGTTTCATCATATTTTAAGGGTAGAACCTTTGCTAAAA
ATAAGATCAGCAGGTCAAAAGGTACAAGATTGTTACTTCTATCAAATTTT
TATGGAAAAAAATGAGAAAGTTGGCGTTCCCACAATTCAGCAGTTGTTAG
AATGGTCTTTTATCAACAGTAACCTGAAATTTGCAGAGGCACCATCATGT
CTGATTATTCAGATGCCTCGATTTGGAAAAGACTTTAAACTATTTAAAAA
AATTTTTCCTTCTCTGGAATTAAATATAACAGATTTACTTGAAGACACTC
CCAGACAGTGCCGGATATGTGGAGGGCTTGCAATGTATGAGTGTAGAGAA
TGCTACGACGATCCGGACATCTCAGCTGGAAAAATCAAGCAGTTTTGTAA
AACCTGCAACACTCAAGTCCACCTTCATCCGAAGAGGCTGAATCATAAAT
ATAACCCAGTGTCACTTCCCAAAGACTTACCCGACTGGGACTGGAGACAC
GGCTGCATCCCTTGCCAGAATATGGAGTTATTTGCTGTTCTCTGCATAGA
AACAAGCCACTATGTTGCTTTTGTGAAGTATGGGAAGGACGATTCTGCCT
GGCTCTTCTTTGACAGCATGGCCGATCGGGATGGTGGTCAGAATGGCTTC
AACATTCCTCAAGTCACCCCATGCCCAGAAGTAGGAGAGTACTTGAAGAT
GTCTCTGGAAGACCTGCATTCCTTGGACTCCAGGAGAATCCAAGGCTGTG
CACGAAGACTGCTTTGTGATGCATATATGTGCATGTACCAGAGTCCAACA
ATGAGTTTGTACAAAGCGGCCGC

Amino Acid Sequence

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMSSGLWSQEKVTSPYWEERIFYLLLQE
CSVTDKQTQKLLKVPKGSIGQYIQDRSVGHSRIPSAKGKKNQIGLKILEQ
PHAVLFVDEKDVVEINEKFTELLLAITNCEERFSLFKNRNRLSKGLQIDV
GCPVKVQLRSGEEKFPGVVRFRGPLLAERTVSGIFFGVELLEEGRGQGFT
DGVYQGKQLFQCDEDCGVFVALDKLELIEDDDTALESDYAGPGDTMQVEL
PPLEINSRVSLKVGETIESGTVIFCDVLPGKESLGYFVGVDMDNPIGNWD
GRFDGVQLCSFACVESTILLHINDIIPESVTQERRPPKLAFMSRGVGDKG
SSSHNKPKATGSTSDPGNRNRSELFYTLNGSSVDSQPQSKSKNTWYIDEV
AEDPAKSLTEISTDFDRSSPPLQPPPVNSLTTENRFHSLPFSLTKMPNTN
GSIGHSPLSLSAQSVMEELNTAPVQESPPLAMPPGNSHGLEVGSLAEVKE
NPPFYGVIRWIGQPPGLNEVLAGLELEDECAGCTDGTFRGTRYFTCALKK
ALFVKLKSCRPDSRFASLQPVSNQIERCNSLAFGGYLSEVVEENTPPKME
KEGLEIMIGKKKGIQGHYNSAYLDSTLFCLFAFSSVLDTVLLRPKEKNDV
EYYSETQELLRTEIVNPLRIYGYVCATKIMKLRKILEKVEAASGFTSEEK
DPEEFLNILFHHILRVEPLLKIRSAGQKVQDCYFYQIFMEKNEKVGVPTI
QQLLEWSFINSNLKFAEAPSCLIIQMPRFGKDFKLFKKIFPSLELNITDL
LEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQFCKTCNTQVHLHPKR
LNHKYNPVSLPKDLPDWDWRHGCIPCQNMELFAVLCIETSHYVAFVKYGK
DDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVGEYLKMSLEDLHSLDSRR
IQGCARRLLCDAYMCMYQSPTMSLYKAAAYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24750
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: harbours a 3 amino acid deletion relative to NCBI ref sequence (Ala305-Ser307). C598 equivalent to C601 in WT full length