cDNA Clone DNA2 (YEAST)

• Plasmid: pRS413 DNA2 D120-S1522(end) D212A D213A L215A I218A (YEAST) 6MYC KITRP 3'UTR
• Parent Plasmid: pRS413
• Expressed: DNA2 D120-S1522(end) D212A D213A L215A I218A (YEAST) 6MYC KITRP 3'UTR
• Genbank: NP_012034.1
• Species: Yeast

Sequence of Insert

Full sequence of cassette
GAGCTCGACGGCCCCATGGAAGAAGTAATATGGAAATATTCACCTTTACA
ACGAGATATGTCCGACAAAACAACAAGCGCTGCCGAATACTCTGATGACT
ACGAAGATGTCCAAAATCCCTCTTCTACACCTATAGTCCCTAATCGACTG
AAAACTGTTCTTAGTTTCACAAATATCCAAGTACCCAACGCTGACGTAAA
TCAACTCATTCAAGAAAATGGAAATGAGCAAGTGCGTCCTAAACCAGCAG
AAATATCTACCAGGGAGTCTCTCCGTAATATAGCTGCTATTGCCGATGAT
GCAGAGGGCGATTTAACTATAAAACCGACGATAACGAAATTCAGCGATTT
GCCATCTTCACCCATCAAGGCACCCAACGTTGAAAAAAAAGCAGAGGTGA
ATGCAGAAGAAGTAGATAAAATGGATTCAACAGGAGATAGCAACGATGGC
GATGACTCTTTGATAGATATTTTAACTCAAAAGTATGTTGAGAAACGCAA
GAGTGAGAGTCAGATAACAATTCAAGGCAACACCAATCAAAAAAGTGGAG
CCCAGGAAAGTTGTGGGAAGAATGATAACACAAAATCGAGAGGAGAAATT
GAAGATCATGAAAATGTAGACAATCAAGCCAAAACAGGCAATGCATTTTA
TGAGAATGAAGAAGACAGTAATTGCCAAAGAATTAAAAAAAATGAAAAAA
TAGAATACAATTCTTCAGACGAATTTTCAGACGATTCACTAATTGAACTA
TTGAACGAAACTCAAACACAAGTGGAACCTAACACTATTGAACAAGATTT
AGACAAAGTGGAGAAAATGGTCTCTGACGATTTGAGAATTGCTACTGATT
CCACTTTATCCGCATATGCTCTCAGAGCTAAGTCTGGTGCTCCAAGAGAC
GGCGTAGTACGATTAGTAATAGTAAGCTTACGTAGTGTTGAATTGCCAAA
AATTGGAACGCAAAAAATACTAGAGTGTATAGACGGCAAAGGCGAACAAA
GTTCTGTTGTCGTTCGACATCCTTGGGTATATTTGGAATTTGAGGTTGGG
GATGTAATACATATTATAGAAGGGAAAAATATTGAAAACAAACGGCTTCT
TTCTGACGACAAAAACCCTAAAACTCAACTGGCTAACGACAACCTGTTGG
TGCTAAACCCTGATGTATTATTTTCGGCAACATCAGTCGGAAGTTCAGTA
GGTTGTTTAAGACGTTCAATTCTGCAAATGCAATTTCAAGATCCCCGCGG
AGAACCAAGCCTTGTCATGACTTTAGGCAATATCGTACACGAGTTATTGC
AAGACTCAATCAAATACAAGCTCTCGCATAATAAAATTTCTATGGAAATC
ATAATACAAAAGCTGGATTCTTTGTTAGAAACTTATTCTTTTTCAATTAT
CATTTGTAATGAAGAAATCCAGTACGTTAAAGAATTAGTTATGAAAGAAC
ATGCTGAAAACATTTTGTACTTCGTCAATAAATTTGTTTCGAAAAGTAAC
TATGGTTGCTACACATCAATTTCCGGAACAAGAAGAACACAGCCAATTTC
TATATCCAATGTGATTGATATTGAAGAAAACATTTGGTCTCCAATATATG
GTCTTAAAGGGTTCCTAGATGCAACTGTTGAAGCTAATGTTGAAAACAAT
AAAAAACACATCGTTCCTTTAGAAGTGAAAACTGGAAAATCCAGAAGCGT
TTCATACGAGGTTCAAGGCCTTATTTATACACTTCTGTTAAATGATCGCT
ATGAGATACCGATAGAGTTTTTTCTTCTTTATTTTACGAGGGATAAAAAT
ATGACAAAGTTCCCTTCTGTTCTGCACTCCATTAAACATATTCTCATGTC
CCGAAATAGAATGAGTATGAATTTTAAGCATCAACTCCAAGAGGTTTTCG
GACAGGCTCAATCAAGGTTTGAATTACCACCCTTACTGCGCGATTCATCT
TGTGATTCATGTTTCATCAAAGAATCATGCATGGTGTTGAATAAGCTACT
AGAGGACGGGACACCAGAAGAAAGTGGCCTGGTAGAAGGTGAGTTTGAAA
TTTTGACAAACCATTTGTCGCAAAATCTCGCCAACTACAAAGAGTTTTTT
ACAAAATATAATGATCTAATAACTAAGGAGGAGTCAAGTATAACATGTGT
AAATAAAGAACTTTTCTTATTGGATGGTTCCACAAGAGAATCCAGAAGTG
GAAGGTGCCTTTCTGGCTTGGTTGTGTCAGAGGTGGTTGAACATGAAAAA
ACAGAGGGAGCGTATATATATTGCTTCTCAAGACGTAGAAATGATAATAA
CTCGCAGTCGATGTTATCATCTCAAATTGCTGCTAATGACTTTGTTATTA
TTAGTGATGAGGAGGGTCATTTTTGCTTGTGCCAAGGTCGTGTACAATTT
ATAAATCCCGCTAAAATTGGTATCTCCGTAAAACGAAAGTTATTAAACAA
TAGGCTTCTAGATAAGGAAAAAGGAGTCACTACCATCCAAAGTGTGGTCG
AATCAGAGCTAGAACAATCCAGTCTCATAGCTACCCAAAATTTGGTCACA
TATAGAATCGACAAAAATGATATCCAACAGAGTTTGTCTTTAGCGCGATT
TAATCTTTTAAGCTTGTTTTTACCAGCTGTTTCACCTGGCGTGGATATTG
TTGACGAAAGGAGTAAATTATGTCGGAAGACAAAGAGATCTGATGGAGGT
AATGAAATACTGCGATCACTTTTAGTTGATAATAGAGCACCTAAGTTTAG
AGATGCAAATGATGATCCCGTCATTCCGTATAAGCTTTCAAAGGATACAA
CATTGAACTTGAATCAAAAAGAAGCAATAGACAAAGTAATGAGGGCCGAA
GATTATGCATTGATATTGGGAATGCCAGGGACTGGTAAGACTACTGTTAT
CGCAGAAATTATTAAAATTCTCGTTTCCGAGGGGAAACGCGTGCTTTTGA
CATCATACACACATTCAGCAGTGGATAATATTTTAATAAAATTGAGGAAC
ACCAACATTAGCATAATGAGATTGGGAATGAAACATAAGGTCCATCCTGA
CACGCAAAAATATGTCCCGAATTACGCGTCAGTGAAAAGTTATAACGATT
ATTTGTCCAAAATTAACAGCACCTCTGTTGTTGCTACCACCTGCTTAGGA
ATTAACGATATACTATTTACACTAAATGAAAAGGATTTTGATTATGTGAT
TTTGGACGAGGCAAGTCAAATTTCAATGCCTGTCGCTTTGGGACCTCTGC
GGTATGGAAATAGGTTTATTATGGTAGGAGATCATTACCAGCTACCGCCA
TTAGTTAAAAATGACGCAGCTCGATTAGGTGGGTTAGAAGAGTCACTGTT
CAAGACTTTCTGTGAAAAGCATCCGGAAAGCGTTGCAGAACTGACTTTAC
AGTACCGTATGTGCGGCGATATTGTAACTTTGTCTAATTTCCTAATTTAT
GATAATAAATTAAAATGCGGGAATAACGAAGTTTTTGCTCAATCTTTAGA
GTTACCAATGCCTGAGGCCTTGTCGCGCTATAGGAATGAATCGGCTAACT
CTAAGCAGTGGCTTGAGGATATACTAGAGCCTACAAGAAAAGTGGTATTT
TTAAATTACGATAACTGCCCGGATATTATCGAACAAAGCGAAAAGGATAA
CATTACGAATCACGGGGAGGCAGAATTGACTCTACAATGTGTGGAAGGAA
TGTTATTGAGCGGCGTACCATGCGAAGATATTGGTGTAATGACGTTATAC
AGAGCACAGTTAAGATTACTGAAGAAGATATTCAACAAAAATGTGTATGA
CGGGCTAGAGATCTTGACTGCTGATCAGTTTCAAGGTCGTGACAAAAAGT
GCATTATTATTTCCATGGTTAGAAGAAATTCTCAATTAAATGGAGGAGCG
CTGCTGAAAGAGCTAAGAAGAGTCAATGTTGCCATGACAAGGGCCAAATC
CAAGCTGATCATTATAGGTTCAAAAAGTACAATCGGCTCTGTTCCTGAAA
TAAAATCGTTCGTGAACCTGTTAGAAGAGCGTAATTGGGTCTATACAATG
TGCAAGGATGCACTCTACAAATACAAATTTCCCGACAGATCAAACGCTAT
TGATGAGGCACGCAAAGGTTGTGGAAAAAGAACAGGTGCCAAACCCATCA
CATCAAAGTCTAAATTCGTAAGCGATAAACCTATCATAAAGGAAATTCTA
CAAGAGTATGAAAGTCGTACGCTGCAGGTCGACTCCGGTTCTGCTGCTAG
TGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGC
TAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAA
GACTTGAACGGATCCTCTAGAGGTGAACAAAAGTTGATTTCTGAAGAAGA
TTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAAC
AAAAATTAATCTCAGAAGAAGACTTGAACGGATCCACTAGCTAAAAAGTG
GAACGATCATTCACTATATATATATCAATTTATATATACGTATGTGTAAT
TGAAGAAAGATACGTTTTTTCCTCTATTGAGAGGCCTGCTGGATGAATAG
CTTTACCTTTTCTAAATCCTTGATACCATCAGTCTCTACTCCTCCACTTA
CATCGACACCGATCGCATTTGGTAACATATTAATGGCAACAGAAACGTTA
TCAGGATTCAATCCACCAGCGATAATGAATTTTATCTCGGGATGACTTGC
AGACCAACTGGAAATTGCACTCCAATTCAATTTCTCACCAGTGCCACCTT
CACCAGAATCGAACAACGTCAGCACATTGTCTACGTGTTCATACAGGTCC
AGTAGTAATTCACAATCCTGTGGGAACTGGAACCTCTTAATGATTGGAAT
TGAAGATGGGATCAAAGATCTGTATTCTTTAATATCTTCATCTCCATGTA
ATTGTATCACATCTAGATTATATTCGTGGTACAGTTGAAGGACATCATCA
ACGGACTGATTTCTAAACACCCCGACTAGTTTAGTACCTTTCACGTTCTC
TTGTTGGTGAACTGCAGTTGAAATACCTTTCGCAACAGATGAGTCAATGG
TTCTTTTCCTACCGGGAACACAAATGATACCTAAGTAATCAGCACCATCA
TCCACAGCAGTCTTTGCAGCTTCAACGGTTTGCAAACCACACACTTTAAC
GAGCATCACAGTAGATTTCGCAAGAGGGTAGCTCGCTCAGAGTACCCAAG
TAAATGATTAGTAAACTGATGTTTGATAGTTCAATTTTTCAATGAAATAA
CCTTATATTAAAATTGATATTACTATTATACAAAAATAAAGAATAAAGGA
TTTGAGTTTATACATAAAATACCATTATTATTTGTTCAGTGAGAGATACC
GGGGTATATGGGATGTGTGTAGTGATACCATGCAATCATGTATCAAACAT
GGAAGAAGTGAAGGGGGAGAAAGAAACTTGAAAAAAAGAAAACCATTTGA
AAAGAGGAAAAGTATCTAATTAAGATCTTTCACCTCTTAGTCTTCTGGCC
TCGAGGCGAGCTCGCATTCATCGATTGACAGGGGGAATAAGAAGAGCTTC
TCCATAACAGGAAAGCTATCACAGCATAAAATATCTATGTCATTTTATAA
AGTTTGGTATATATAACACGTAATATTAGCATCTGAGGTTCGAAAGCTCG
CATGTGTGTCTTTTCTAAAAAGTTTTCGTTTGAACTTTTCCAAAAGCTTC
CTCATTAACGAACCAGGATGTTTTTTCACCAAATAATTTGTTAACCAATG
CGGTTGGCAATTGATCGTTTTTCAAGTCAAAGATCTCATGCATTATATTT
TGTTTGGAACTGCCCTCAGCCACAAAACACAGGGCTTTGGCGGGGCCC

Amino Acid Sequence

DGPMEEVIWKYSPLQRDMSDKTTSAAEYSDDYEDVQNPSSTPIVPNRLKTVLSFTNIQVPNADVNQLIQENGNEQVRPKPAEISTRESLRNIAAIADDAEGDLTIKPTITKFSDLPSSPIKAPNVEKKAEVNAEEVDKMDSTGDSNDGDDSLIDILTQKYVEKRKSESQITIQGNTNQKSGAQESCGKNDNTKSRGEIEDHENVDNQAKTGNAFYENEEDSNCQRIKKNEKIEYNSSDEFSDDSLIELLNETQTQVEPNTIEQDLDKVEKMVSDDLRIATDSTLSAYALRAKSGAPRDGVVRLVIVSLRSVELPKIGTQKILECIDGKGEQSSVVVRHPWVYLEFEVGDVIHIIEGKNIENKRLLSDDKNPKTQLANDNLLVLNPDVLFSATSVGSSVGCLRRSILQMQFQDPRGEPSLVMTLGNIVHELLQDSIKYKLSHNKISMEIIIQKLDSLLETYSFSIIICNEEIQYVKELVMKEHAENILYFVNKFVSKSNYGCYTSISGTRRTQPISISNVIDIEENIWSPIYGLKGFLDATVEANVENNKKHIVPLEVKTGKSRSVSYEVQGLIYTLLLNDRYEIPIEFFLLYFTRDKNMTKFPSVLHSIKHILMSRNRMSMNFKHQLQEVFGQAQSRFELPPLLRDSSCDSCFIKESCMVLNKLLEDGTPEESGLVEGEFEILTNHLSQNLANYKEFFTKYNDLITKEESSITCVNKELFLLDGSTRESRSGRCLSGLVVSEVVEHEKTEGAYIYCFSRRRNDNNSQSMLSSQIAANDFVIISDEEGHFCLCQGRVQFINPAKIGISVKRKLLNNRLLDKEKGVTTIQSVVESELEQSSLIATQNLVTYRIDKNDIQQSLSLARFNLLSLFLPAVSPGVDIVDERSKLCRKTKRSDGGNEILRSLLVDNRAPKFRDANDDPVIPYKLSKDTTLNLNQKEAIDKVMRAEDYALILGMPGTGKTTVIAEIIKILVSEGKRVLLTSYTHSAVDNILIKLRNTNISIMRLGMKHKVHPDTQKYVPNYASVKSYNDYLSKINSTSVVATTCLGINDILFTLNEKDFDYVILDEASQISMPVALGPLRYGNRFIMVGDHYQLPPLVKNDAARLGGLEESLFKTFCEKHPESVAELTLQYRMCGDIVTLSNFLIYDNKLKCGNNEVFAQSLELPMPEALSRYRNESANSKQWLEDILEPTRKVVFLNYDNCPDIIEQSEKDNITNHGEAELTLQCVEGMLLSGVPCEDIGVMTLYRAQLRLLKKIFNKNVYDGLEILTADQFQGRDKKCIIISMVRRNSQLNGGALLKELRRVNVAMTRAKSKLIIIGSKSTIGSVPEIKSFVNLLEERNWVYTMCKDALYKYKFPDRSNAIDEARKGCGKRTGAKPITSKSKFVSDKPIIKEILQEYESRTLQVDSGSAASGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGSSRGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGSTS*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU26078
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Linker between end of DNA2 and 6myc has been deleted.; The full cassette of DNA2 120-end D212A D213A L215A I218A 6myc KITRP1 3'UTR is in the sequence of insert field.; Use as template Internal Sac1 Site needs to be mutated