cDNA Clone mSIK3 (mouse)

• Plasmid: pCMV HA mSIK3
• Parent Plasmid: pCMV HA
• Expressed: HA-MOUSE SIK3
• Genbank: Q6P4S6
• Tag: HA

Sequence of Insert

CTCGAGGTCGACGGTATCGATAAGCTTGATATCCACTGTGGAATTCGCCCTTCAAGATCTATGGCCGCTCGCATCGGCTACTACGAGATCGACCGCACCATCGGCAAGGGCAACTTCGCTGTGGTCAAGCGGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTATTAAAATCATAGATAAGAGCCAGCTGGATGAAGAAAACTTGAAGAAGATTTTCCGGGAGGTTCAGATAATGAAGATGCTTTGCCATCCACACATCATCAGACTCTACCAGGTCATGGAGACAGAGCGCATGATTTACCTGGTGACAGAATACGCTAGCGGAGGGGAGATATTTGACCACTTGGTAGCCCATGGAAGAATGGCAGAGAAGGAAGCTCGACGGAAGTTCAAACAGATCGTCACAGCGGTGTATTTTTGTCACTGTCGGAATATCGTTCATCGTGATTTAAAAGCCGAAAACTTACTTCTGGACGCCAATCTGAATATCAAAATAGCAGACTTTGGCTTCAGCAACCTCTTCACTCCAGGGCAGCTGCTGAAGACGTGGTGTGGCAGCCCTCCCTATGCCGCCCCAGAGCTCTTCGAAGGGAAGGAATATGATGGGCCCAAAGTGGACATATGGAGCCTTGGAGTTGTCCTCTATGTGCTGGTGTGTGGCGCCCTGCCGTTTGATGGGAGCACACTGCAGAATCTGCGGGCCCGCGTGCTGAGTGGCAAGTTCCGCATCCCGTTCTTTATGTCCACAGAGTGTGAGCACTTGATCCGCCACATGCTGGTGTTAGATCCAAATAAGCGCCTCTCAATGGAACAGATCTGCAGGCACAAGTGGATGAAGCTCGGAGATGCAGACCCCAACTTTGACAGGTTAATAGCGGAGTGCCAGCAACTGAAGGAGGAAAGGCAGTCGGACCCCCTCAACGATGATGTCCTCTTGGCTATGGAAGACATGGGGCTGGACAAGGAGCGCACACTTCAGTCATTAAGGTCAGATGCCTATGACCACTATAGTGCAATCTACAGCCTGCTGTGCGATCGACACAAGAAACATAAAACTCTGCGTCCCGGAGCACTTCCCAGCATGCCCCAAGCCATGACTTTCCAGGCACCAGTCAATCTCCAGGCGGAGCAGACAGGCACTGCTATGAACCTTAGCGTCCCTCAAGTTCAGCTGATCAACCCAGAGAACCAAATTATAGAGCCTGACGGGGCTGTGAACTTGGACAGTGATGAGGGTGAAGAGCCTTCTCCAGAAGCCTTGGTTCGCTATTTGTCAATGAGGAGGCACACGGTGGGAGTGGCTGACCCACGCACGGAAGTTATGGAAGATCTGCAGAAGCTGCTGCCTGGCTTTCCTGGAGTAAACCCACAGGGTCCATTCCTACAAGTGGCCCCTAACATGAACTTCACGCACAACCTGTTGCCCATGCAGAGTCTGCAGCCAACTGGGCAGCTTGAGTACAAGGAACAGTCTCTGTTACAGCCGCCTACACTACAGCTACTGAACGGAATGGGGCCCCTTGGCCGGAGAGCCTCAGATGGAGGCGCCAACATCCAACTGCATGCCCAGCAGCTGCTCAAGCGCCCACGGGGACCGTCCCCACTTGTCACCATGACACCAGCAGTGCCAGCAGTTACCCCTGTGGATGAGGAGAGCTCGGATGGGGAGCCAGATCAGGAAGCTGTGCAGAGGTACTTGGCAAATAGGTCCAAAAGGCACACACTGGCCATGACCAGCCCGACAGCTGAGATCCCACCGGACCTGCAACGGCAGCTAGGACAACAGTCTTTCCGTTCCCGGGTCTGGCCTCCTCACCTGGTACCTGACCAGCATCGCTCCACCTACAAGGACTCCAACACCCTGCATCTCCCTACGGAGCGTTTCTCCCCCGTGCGCCGGTTCTCAGACGGGGCTGCAAGCATCCAGGCCTTCAAAGCTCACCTGGAAAAAATGGGCAACAGCAGCAGCATCAAGCAGTTGCAGCAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGGGGCAGGTGGATGAGAGGACCCTGGAGAAGACCCAGCAGCAGCATATGTTGTACCAGCAGGAGCAGCACCATCAGATCCTCCAGCAGCAAATCCAAGATTCTATTTGTCCTCCTCAGCCCTCCCCACCTCTTCAGGTTGCCTGTGAAAACCAACCAGCCCTCCTCACCCACCAGCTCCAGAGGTTAAGGATTCAACCTTCAAGCCCACCCCCCAACCATCCCAGCAACCATCTCTTCAGACAGCCAAGTAACAGTCCTCCCCCGGTCAGCAGTGCCATGATCACGTCTCACGGTGCTACGTCTCCCTCCCAGTTTCAAGGCTTACCCTCCCATGGTGCAATCTTCCAGCAGCAACCCGAGAACTGTTCCCCGCCTCCCAGTGTAGCACTAACCTGCCTGGGCCTACAGCAGGCTAGCCAGTCACAGCCAGTGACTATCCAGCTACAGGAGCCAGTTGACATGCTCAGCAACATGGCCGGAACCGCGGCAGGCTCTGCAGGGCGGAGCATCCCCATCAGCCCCAGCGCCAGTCAGATTCAGATACAGCACCGAGCCAGCCTAATGGCTCCCTTCAGCTATGGGCACCGGCCCTTGTCCAAGCAGCTGAGCGCTGACAGTGCAGAGGCCCACAGCTTGAACATGAATCGGTTCTCCCCTGCCAACTACGACCAGGCGCATTTACACCCCCATCTGTTTTCGGACCAGTCCCGAGGTTCCCCCAGCAGCTACAGCCCTTCAACAGGAGTGGGGTTTCCTCCAACTCAAGCCCTGAAAGTTCCTCCGCTTGACCAGTTCCCCACTTTCCCTCCCAGTGCCCAGCAGCAGCCACCCCACTATACCACGTCAGCACTACAGCAGGCCCTGCTATCTCCTACACCGCCAGACTATCCCCGACACCAGCAGGTTCCCCATATCCTTCAAGGACTGCTCTCTCCCCGGCATTCACTCACCGGCCACTCGGACATTCGGCTGCCTCCGGCAGAGTTTGCACAGCTCATCAAAAGGCAGCAACAGCATCGACAGCAGCAGCAGCAGCAGCAGCAGCAACAAGAATACCATGAATTGTTCAGGCACATGAACCAAGGGGATGCTGTTAGCCTAGCTCCCAGCCTCGGGGGACAGAATATGACAGAGCAACAGGCTTTATCTTATCAAAATGCTGACTCGTACCACCGCCACCACACCAGCCCCCAGCATATCTTACAGATCAGGGCGCAAGATTGTATCTCACAGGGTCCCTCGCCCACCCCCACCCATGGCTATGCCCATCAGCCACCACTAATGCATTCGGAGAGTATGGAGGAAGACTGCTTGTGTGAGGGGCTCAAGGAGGGCTTCCCAGACAAGAGCTCAAGCACACTGACCAAAGGTTGCCACAACAGCCCTCTGCTCTTGTGTACCAGTGGGCCTGGGGACCCTGAGCCTTTGCTGGGAACTGTGAGTCAGGCCCGGGAGCTGGGGATCCATCCCTACGGACACCAGCCAACTGCCACCACGTTCAGTAGAAATAAGGTGCCCAGCCGAGAGTCTGTCCTAGGGAACTGCCTGGAAAGAAGTTCTCCTGGACAAGCAATGGAGCTGCCGGATCACAACGGCCTTGGGTACCCAGTACGGCCCTTAGTCAGTGAGCACCTCAGGTCCCGGACGCTCCAGAGACACCACACGATCCAGAACAGCGACGATGCCTATGTACAGCTGGATACCTTGCCAGGAATGAGCCTGGTGGCAGGCAAGGCGCTGAGCTCTGCCCGGATGTCAGATGCAGTTCTTAGTCAGTCTTCACTCATGGGCAGCCAACAGTTTCAGGATGAGGAAGATGAAGAATGTGGGGTGAGCCTGGGCCACGAGCATCCAGGCCTGGGTGATGGCAGCCAGCATCTCAACTCCTCTCGCTATCCAGCTACGTGTGTTACAGACATCATGCTCAGCCACAAGCACCCAGAGGTCTCCTTCAGCATGGAGCAAGCCGGCGTGTAGgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPGEFASLEVDGIDKLDIHCGIRPSRSMAARIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKSQLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDANLNIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQICRHKWMKLGDADPNFDRLIAECQQLKEERQSDPLNDDVLLAMEDMGLDKERTLQSLRSDAYDHYSAIYSLLCDRHKKHKTLRPGALPSMPQAMTFQAPVNLQAEQTGTAMNLSVPQVQLINPENQIIEPDGAVNLDSDEGEEPSPEALVRYLSMRRHTVGVADPRTEVMEDLQKLLPGFPGVNPQGPFLQVAPNMNFTHNLLPMQSLQPTGQLEYKEQSLLQPPTLQLLNGMGPLGRRASDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQEAVQRYLANRSKRHTLAMTSPTAEIPPDLQRQLGQQSFRSRVWPPHLVPDQHRSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLEKMGNSSSIKQLQQECEQLQKMYGGQVDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICPPQPSPPLQVACENQPALLTHQLQRLRIQPSSPPPNHPSNHLFRQPSNSPPPVSSAMITSHGATSPSQFQGLPSHGAIFQQQPENCSPPPSVALTCLGLQQASQSQPVTIQLQEPVDMLSNMAGTAAGSAGRSIPISPSASQIQIQHRASLMAPFSYGHRPLSKQLSADSAEAHSLNMNRFSPANYDQAHLHPHLFSDQSRGSPSSYSPSTGVGFPPTQALKVPPLDQFPTFPPSAQQQPPHYTTSALQQALLSPTPPDYPRHQQVPHILQGLLSPRHSLTGHSDIRLPPAEFAQLIKRQQQHRQQQQQQQQQQEYHELFRHMNQGDAVSLAPSLGGQNMTEQQALSYQNADSYHRHHTSPQHILQIRAQDCISQGPSPTPTHGYAHQPPLMHSESMEEDCLCEGLKEGFPDKSSSTLTKGCHNSPLLLCTSGPGDPEPLLGTVSQARELGIHPYGHQPTATTFSRNKVPSRESVLGNCLERSSPGQAMELPDHNGLGYPVRPLVSEHLRSRTLQRHHTIQNSDDAYVQLDTLPGMSLVAGKALSSARMSDAVLSQSSLMGSQQFQDEEDEECGVSLGHEHPGLGDGSQHLNSSRYPATCVTDIMLSHKHPEVSFSMEQAGV

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU47397
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: sequence starts at Met59 compared to NM_027498.3, mutations Q1032H and S1277N compared to NM_027498.3; similar to human variant, the longer version was not obtainable through RT or commercially; used Not further down in pSCB.