cDNA Clone NLEL

• Synonyms: secreted effector protein
• Plasmid: pGEX-TEV-NLEL (N59-end) C753S
• Parent Plasmid: pGEX TEV
• Expressed: GST-TEV-NLEL N59- end C753S
• Genbank: NP_309587.1
• Tag: GST
• Species: Escherichia coli

Sequence of Insert

GGATCCAACGGCGAAACCCTGAGCATTAGCGAACCGATTACCACCCTGCC
GGATCTGCTGCCGGGCAGCCTGAAAGAACTGGTGCTGAACGGCTGCACCG
AACTGAAAAGCATTAACTGCCTGCCGCCGAACCTGAGCAGCCTGAGCATG
GTGGGCTGCAGCAGCCTGGAAGTGATTAACTGCAGCATTCCGGAAAACGT
GATTAACCTGAGCCTGTGCCATTGCAGCAGCCTGAAACATATTGAAGGCA
GCTTTCCGGAAGCGCTGCGCAACAGCGTGTATCTGAACGGCTGCAACAGC
CTGAACGAAAGCCAGTGCCAGTTTCTGGCGTATGATGTGAGCCAGGGCCG
CGCGTGCCTGAGCAAAGCGGAACTGACCGCGGATCTGATTTGGCTGAGCG
CGAACCGCACCGGCGAAGAAAGCGCGGAAGAACTGAACTATAGCGGCTGC
GATCTGAGCGGCCTGAGCCTGGTGGGCCTGAACCTGAGCAGCGTGAACTT
TAGCGGCGCGGTGCTGGATGATACCGATCTGCGCATGAGCGATCTGAGCC
AGGCGGTGCTGGAAAACTGCAGCTTTAAAAACAGCATTCTGAACGAATGC
AACTTTTGCTATGCGAACCTGAGCAACTGCATTATTCGCGCGCTGTTTGA
AAACAGCAACTTTAGCAACAGCAACCTGAAAAACGCGAGCTTTAAAGGCA
GCAGCTATATTCAGTATCCGCCGATTCTGAACGAAGCGGATCTGACCGGC
GCGATTATTATTCCGGGCATGGTGCTGAGCGGCGCGATTCTGGGCGATGT
GAAAGAACTGTTTAGCGAAAAAAGCAACACCATTAACCTGGGCGGCTGCT
ATATTGATCTGAGCGATATTCAGGAAAACATTCTGAGCGTGCTGGATAAC
TATACCAAAAGCAACAAAAGCATTCTGCTGACCATGAACACCAGCGATGA
TAAATATAACCATGATAAAGTGCGCGCGGCGGAAGAACTGATTAAAAAAA
TTAGCCTGGATGAACTGGCGGCGTTTCGCCCGTATGTGAAAATGAGCCTG
GCGGATAGCTTTAGCATTCATCCGTATCTGAACAACGCGAACATTCAGCA
GTGGCTGGAACCGATTTGCGATGATTTTTTTGATACCATTATGAGCTGGT
TTAACAACAGCATTATGATGTATATGGAAAACGGCAGCCTGCTGCAGGCG
GGCATGTATTTTGAACGCCATCCGGGCGCGATGGTGAGCTATAACAGCAG
CTTTATTCAGATTGTGATGAACGGCAGCCGCCGCGATGGCATGCAGGAAC
GCTTTCGCGAACTGTATGAAGTGTATCTGAAAAACGAAAAAGTGTATCCG
GTGACCCAGCAGAGCGATTTTGGCCTGTGCGATGGCAGCGGCAAACCGGA
TTGGGATGATGATAGCGATCTGGCGTATAACTGGGTGCTGCTGAGCAGCC
AGGATGATGGCATGGCGATGATGTGCAGCCTGAGCCATATGGTGGATATG
CTGAGCCCGAACACCAGCACCAACTGGATGAGCTTTTTTCTGTATAAAGA
TGGCGAAGTGCAGAACACCTTTGGCTATAGCCTGAGCAACCTGTTTAGCG
AAAGCTTTCCGATTTTTAGCATTCCGTATCATAAAGCGTTTAGCCAGAAC
TTTGTGAGCGGCATTCTGGATATTCTGATTAGCGATAACGAACTGAAAGA
ACGCTTTATTGAAGCGCTGAACAGCAACAAAAGCGATTATAAAATGATTG
CGGATGATCAGCAGCGCAAACTGGCGTGCGTGTGGAACCCGTTTCTGGAT
GGCTGGGAACTGAACGCGCAGCATGTGGATATGATTATGGGCAGCCATGT
GCTGAAAGATATGCCGCTGCGCAAACAGGCGGAAATTCTGTTTTGCCTGG
GCGGCGTGTTTTGCAAATATAGCAGCAGCGATATGTTTGGCACCGAATAT
GATAGCCCGGAAATTCTGCGCCGCTATGCGAACGGCCTGATTGAACAGGC
GTATAAAACCGATCCGCAGGTGTTTGGCAGCGTGTATTATTATAACGATA
TTCTGGATCGCCTGCAGGGCCGCAACAACGTGTTTACCTcCACCGCGGTG
CTGACCGATATGCTGACCGAACATGCGAAAGAAAGCTTTCCGGAAATTTT
TAGCCTGTATTATCCGGTGGCGTGGCGCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSNGETLSISEPITTLPDLLPGS
LKELVLNGCTELKSINCLPPNLSSLSMVGCSSLEVINCSIPENVINLSLC
HCSSLKHIEGSFPEALRNSVYLNGCNSLNESQCQFLAYDVSQGRACLSKA
ELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNLSSVNFSGAVLD
DTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSNFSN
SNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSE
KSNTINLGGCYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDK
VRAAEELIKKISLDELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPIC
DDFFDTIMSWFNNSIMMYMENGSLLQAGMYFERHPGAMVSYNSSFIQIVM
NGSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSD
LAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNT
FGYSLSNLFSESFPIFSIPYHKAFSQNFVSGILDILISDNELKERFIEAL
NSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPL
RKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQ
VFGSVYYYNDILDRLQGRNNVFTSTAVLTDMLTEHAKESFPEIFSLYYPV
AWR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24819
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: as described in http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0019331. Codon optimised for E. coli