cDNA Clone Kazachoc

• Synonyms: BEST:CK01510; CG2768; CG5594; CK01510; Dmel\CG5594; E(sda)J; KCC; l(2)60A-C; Line J
• Plasmid: pSCB Kazachoc C-FLAG Xho template only
• Parent Plasmid: pSC-B
• Genbank: AAF47098
• Species: Drosophila Melanogaster

Sequence of Insert

CTCGAGATGCCAGATAGATTTCAAGTTACAAAGGCTGACGAGGACACCGCGTTGGACTACAACCAGGATGAGTCTGCGAGCGGCAAGCTACTGGGGGACATCCACGACGAAACCCTAGGTGAGAACTATGGGTCGTACGATGACTCAGGTGATATCCACAGGGCGGAGGAGAACCAGAAGTCCAGCATCGACCCAAATCTATATCTGTACGACGATGATTTGGAGACCAGGCCCCATATATCAACGTTCATTTCATCAATTGCCAATTATGAGAACACGATACCAGCGGCCACCGATCCCGATGCAAAGCCGGCAGCTCCATCGGCTCGAATGGGTACCCTAATTGGAGTGTTCTTGCCATGCATTCAAAACATCTTTGGTGTCATATTGTTCATTCGGTTAACATGGGTTGTTGGAACGGCTGGCGCCGTGTGTGGATTCCTGATTGTTCTGACCTGCTGCTGTGTGACCATGCTAACCGCGATCTCGATGTCGGCCATTGCAACGAACGGGGTGGTTCCGGCGGGCGGGAGTTACTTCATGATATCACGATCCCTGGGCCCTGAATTCGGCGGAGCGGTGGGAATGTTGTTCTATACGGGCACCACGCTAGCAGCGGCGATGTACATCGTTGGTGCCGTGGAAATCGTATTGCACTCATTAATTATTCATTGCAGACATACATGGCGCCGTGGGCATCGATATTTGGGGACTTCACCAAGGACGCTGACGCGATGTACAACAATTTCAGGGTCTACGGCACTTTGCTGCTCATCTTTATGGGTCTCATTGTGTTCCTGGGCGTGAAATTCGTCAATAAGTTCGCGACGGTGGCCCTGGCCTGCGTCATCCTTTCGATAATAGCGGTGTATGTGGGAATATTTGACAATATCCATGGCAACGAAAAGCTATACATGTGTGTTCTGGGAAAACGACTCTTGAAGGATATCCCGCTGGAGAATTGCACAAAGGAAGACTCCTTCTTACGCGACATATACTGCCCAGATGGTAAATGCGAGGAATACTACCTGGCCAATAACGTGACCAAAGTCAAGGGCATCAAGGGTTTGGCCAGTGGCGTGTTCTACGACAACATCTTCCCTTCGTTCCTGGAGAAGGGCCAGTTCATATCCTACGGCAAGAGTGCAATTGACATTGAGAACACTAGTGGAGAGTCATACAACCAGATCATGGCCGACATTACCACGTCGTTCACCCTTCTTATCGGCATCTTCTTCCCATCGGTCACAGGCATCATGGCTGGATCCAATCGATCGGGCGACCTGGCTGACGCCCAGAAGAGCATACCCATCGGAACGATATGTGCCATTCTGACCACCAGCACAGTCTACTTGTCCAGCGTTATGTTCTTCGCCGGCACAGTGGACAATCTCCTGTTGAGGGACAAATTCGGTCAGTCTATTGGTGGCAAGCTGGTGGTGGCCAATATTGCCTGGCCAAATCAGTGGGTCATTCTGATTGGCTCCTTCCTCTCCACCTTGGGCGCTGGTCTGCAGAGTTTGACTGGAGCACCCCGACTGCTGCAGGCGATTGCCAGGGATGAGATCATCCCCTTCCTGGCTCCATTTGCCAAGTCTTCGAAGCGTGGCGAACCCACCCGTGCACTGCTCCTGACCATTGTCATTTGCCAGTGCGGCATCCTGTTGGGCAACGTGGACTTGCTGGCTCCTCTGCTGTCCATGTTCTTCCTCATGTGCTACGGCTTTGTCAACCTGGCCTGCGCTGTGCAAACCCTGCTGAGGACTCCCAACTGGAGACCGCGCTTCAAGTTCTACCACTGGAGCTTGTCGCTCATCGGCCTGACGCTGTGCATATCAGTCATGATCATGACTTCCTGGTATTTCGCACTGATTGCTATGGGAATGGCCATCATTATCTACAAATACATAGAGTACCGCGGTGCTGAGAAGGAGTGGGGTGATGGCATTCGTGGAATGGCCCTGACCGCCGCCAGGTACTCGCTACTCCGTCTGGAGGAAGGCCCACCGCATACGAAAAATTGGCGTCCCCAAATTTTGGTGCTTTCGAAGCTCAACGACAACCTCTTGCCAAAATACAGGAAGATTTTCTCCTTTGCCACCCAGCTGAAAGCTGGCAAGGGATTGACGATTTGTGTGTCTGTGATAAAGGGCGACCACACTAAGATCACTAACAAAGCCGTGGATGCGAAGGCCACGCTGCGCAAGTACATGACCGACGAGAAGGTGAAGGGCTTCTGTGATGTCCTGGTAGCCCAGCAGATCGGCGAGGGCCTCAGCTCAGTCATCCAAACCATCGGACTGGGGGGCATGAAGCCCAACACAGTCATCATCGGATGGCCGTACAGCTGGCGGCAGGAGGGCAGGAACAGCTGGAAGACCTTCATCCAAACGGTCCGCACGGTGGCCGCCTGCCACATGGCCCTCATGGTGCCCAAGGGCATCAACTTCTATCCAGAATCAAACCACAAAATCGGTGGCAACATTGATATCTGGTGGATTGTCCACGACGGTGGTCTGCTCATGTTGCTGCCCTTCCTGCTGAAGCAACACCGCACCTGGCGCAATTGCAAGCTAAGGATCTTCACAGTTGCTCAAATCGAGGACAACTCGATTCAAATGAAGAAGGATCTAAAGACATTCCTCTACCATCTTCGAATCGAGGCCGATGTTGAAGTTGTTGAGATGAACAACAGCGATATTTCCGCTTATACCTACGAGCGGACCCTGATGATGGAACAGCGTAATCAGATGCTGAGAGCATTAGGCTTAAATAAGAAAGAAAACTCCAAAGTGGTTCAAACAATTGTAGACCACCATTATGACGCCACCAAAACGGCGTCTAAAGTTCGCTTCGCCGATCCAACTATAGAAGAAACACAGCATCACGATTCTCAAAACGACGAGAAACGTAACTCAATTGATTTGGATGGTCCCGAGAACGCGGACACACCCGAAACTACTTCTAACAAGGATGAATCGACAGAGAAAGCCGACGGAGACTTCAAGTCCAGTGTGAAACCGGATGAGTTCAACGTTCGTCGCATGCACACAGCAATAAAACTAAACGAGGTTATTGTAGAAAAGTCACAGGACGCCCAGTTGGTCATAATGAATCTACCTGGACCACCTAGGGAAGTGAGAGCGGAGCGTGAAAGAAATTATATGGAATTTCTGGAGGTATTAACAGAGGGCCTTGAAAAAGTGTTAATGGTTCGCGGAGGAGGCCGAGAAGTTATAACAATTTACTCTGACTACAAGGACGATGACGATAAGTAACTCGAG

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48956
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains an extra exon as compared to the main variant, this is in-frame so is likely to be naturally occurring transcript. This exon is 31 residues in length and appears between C922 and D923. A smaller insertion upstream (between L216 and T217) requires removal to yield isoformD.; Several silent changes as compared to the reference sequence; G>C 1527, G>A1596, A>T 2058, T>C 2235, A>G 2325, T>C 2451 and T>C 2523 bp.