cDNA Clone Kazachoc

• Synonyms: BEST:CK01510; CG2768; CG5594; CK01510; Dmel\CG5594; E(sda)J; KCC; l(2)60A-C; Line J
• Plasmid: pTREG KAZACHOC IsoD C-FLAG
• Parent Plasmid: pTREG Mod
• Expressed: KAZACHOC IsoD C-FLAG
• Genbank: AAF47098
• Tag: FLAG
• Species: Drosophila Melanogaster

Sequence of Insert

CTCGAGATGCCAGATAGATTTCAAGTTACAAAGGCTGACGAGGACACCGCGTTGGACTACAACCAGGATGAGTCTGCGAGCGGCAAGCTACTGGGGGACATCCACGACGAAACCCTAGGTGAGAACTATGGGTCGTACGATGACTCAGGTGATATCCACAGGGCGGAGGAGAACCAGAAGTCCAGCATCGACCCAAATCTATATCTGTACGACGATGATTTGGAGACCAGGCCCCATATATCAACGTTCATTTCATCAATTGCCAATTATGAGAACACGATACCAGCGGCCACCGATCCCGATGCAAAGCCGGCAGCTCCATCGGCTCGAATGGGTACCCTAATTGGAGTGTTCTTGCCATGCATTCAAAACATCTTTGGTGTCATATTGTTCATTCGGTTAACATGGGTTGTTGGAACGGCTGGCGCCGTGTGTGGATTCCTGATTGTTCTGACCTGCTGCTGTGTGACCATGCTAACCGCGATCTCGATGTCGGCCATTGCAACGAACGGGGTGGTTCCGGCGGGCGGGAGTTACTTCATGATATCACGATCCCTGGGCCCTGAATTCGGCGGAGCGGTGGGAATGTTGTTCTATACGGGCACCACGCTAGCAGCGGCGATGTACATCGTTGGTGCCGTGGAAATCGTATTGACATACATGGCGCCGTGGGCATCGATATTTGGGGACTTCACCAAGGACGCTGACGCGATGTACAACAATTTCAGGGTCTACGGCACTTTGCTGCTCATCTTTATGGGTCTCATTGTGTTCCTGGGCGTGAAATTCGTCAATAAGTTCGCGACGGTGGCCCTGGCCTGCGTCATCCTTTCGATAATAGCGGTGTATGTGGGAATATTTGACAATATCCATGGCAACGAAAAGCTATACATGTGTGTTCTGGGAAAACGACTCTTGAAGGATATCCCGCTGGAGAATTGCACAAAGGAAGACTCCTTCTTACGCGACATATACTGCCCAGATGGTAAATGCGAGGAATACTACCTGGCCAATAACGTGACCAAAGTCAAGGGCATCAAGGGTTTGGCCAGTGGCGTGTTCTACGACAACATCTTCCCTTCGTTCCTGGAGAAGGGCCAGTTCATATCCTACGGCAAGAGTGCAATTGACATTGAGAACACTAGTGGAGAGTCATACAACCAGATCATGGCCGACATTACCACGTCGTTCACCCTTCTTATCGGCATCTTCTTCCCATCGGTCACAGGCATCATGGCTGGATCCAATCGATCGGGCGACCTGGCTGACGCCCAGAAGAGCATACCCATCGGAACGATATGTGCCATTCTGACCACCAGCACAGTCTACTTGTCCAGCGTTATGTTCTTCGCCGGCACAGTGGACAATCTCCTGTTGAGGGACAAATTCGGTCAGTCTATTGGTGGCAAGCTGGTGGTGGCCAATATTGCCTGGCCAAATCAGTGGGTCATTCTGATTGGCTCCTTCCTCTCCACCTTGGGCGCTGGTCTGCAGAGTTTGACTGGAGCACCCCGACTGCTGCAGGCGATTGCCAGGGATGAGATCATCCCCTTCCTGGCTCCATTTGCCAAGTCTTCGAAGCGTGGCGAACCCACCCGTGCACTGCTCCTGACCATTGTCATTTGCCAGTGCGGCATCCTGTTGGGCAACGTGGACTTGCTGGCTCCTCTGCTGTCCATGTTCTTCCTCATGTGCTACGGCTTTGTCAACCTGGCCTGCGCTGTGCAAACCCTGCTGAGGACTCCCAACTGGAGACCGCGCTTCAAGTTCTACCACTGGAGCTTGTCGCTCATCGGCCTGACGCTGTGCATATCAGTCATGATCATGACTTCCTGGTATTTCGCACTGATTGCTATGGGAATGGCCATCATTATCTACAAATACATAGAGTACCGCGGTGCTGAGAAGGAGTGGGGTGATGGCATTCGTGGAATGGCCCTGACCGCCGCCAGGTACTCGCTACTCCGTCTGGAGGAAGGCCCACCGCATACGAAAAATTGGCGTCCCCAAATTTTGGTGCTTTCGAAGCTCAACGACAACCTCTTGCCAAAATACAGGAAGATTTTCTCCTTTGCCACCCAGCTGAAAGCTGGCAAGGGATTGACGATTTGTGTGTCTGTGATAAAGGGCGACCACACTAAGATCACTAACAAAGCCGTGGATGCGAAGGCCACGCTGCGCAAGTACATGACCGACGAGAAGGTGAAGGGCTTCTGTGATGTCCTGGTAGCCCAGCAGATCGGCGAGGGCCTCAGCTCAGTCATCCAAACCATCGGACTGGGGGGCATGAAGCCCAACACAGTCATCATCGGATGGCCGTACAGCTGGCGGCAGGAGGGCAGGAACAGCTGGAAGACCTTCATCCAAACGGTCCGCACGGTGGCCGCCTGCCACATGGCCCTCATGGTGCCCAAGGGCATCAACTTCTATCCAGAATCAAACCACAAAATCGGTGGCAACATTGATATCTGGTGGATTGTCCACGACGGTGGTCTGCTCATGTTGCTGCCCTTCCTGCTGAAGCAACACCGCACCTGGCGCAATTGCAAGCTAAGGATCTTCACAGTTGCTCAAATCGAGGACAACTCGATTCAAATGAAGAAGGATCTAAAGACATTCCTCTACCATCTTCGAATCGAGGCCGATGTTGAAGTTGTTGAGATGAACAACAGCGATATTTCCGCTTATACCTACGAGCGGACCCTGATGATGGAACAGCGTAATCAGATGCTGAGAGCATTAGGCTTAAATAAGAAAGAAAACTCCAAAGTGGTTCAAACAATTGTAGACCACCATTATGACGCCACCAAAACGGCGTCTAAAGTTCGCTTCGCCGATCCAACTATAGAAGAAACACAGCATCACGATTCTCAAAACGACGAGAAACGTAACTCAATTGATTTGGATGGTCCCGAGAACGCGGACACACCCGAAACTACTTCTAACAAGGATGAATCGACAGAGAAAGCCGACGGAGACTTCAAGTCCAGTGTGAAACCGGATGAGTTCAACGTTCGTCGCATGCACACAGCAATAAAACTAAACGAGGTTATTGTAGAAAAGTCACAGGACGCCCAGTTGGTCATAATGAATCTACCTGGACCACCTAGGGAAGTGAGAGCGGAGCGTGAAAGAAATTATATGGAATTTCTGGAGGTATTAACAGAGGGCCTTGAAAAAGTGTTAATGGTTCGCGGAGGAGGCCGAGAAGTTATAACAATTTACTCTGACTACAAGGACGATGACGATAAGTAACTCGAG

Amino Acid Sequence

MPDRFQVTKADEDTALDYNQDESASGKLLGDIHDETLGENYGSYDDSGDI
HRAEENQKSSIDPNLYLYDDDLETRPHISTFISSIANYENTIPAATDPDA
KPAAPSARMGTLIGVFLPCIQNIFGVILFIRLTWVVGTAGAVCGFLIVLT
CCCVTMLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGMLFYTGT
TLAAAMYIVGAVEIVLTYMAPWASIFGDFTKDADAMYNNFRVYGTLLLIF
MGLIVFLGVKFVNKFATVALACVILSIIAVYVGIFDNIHGNEKLYMCVLG
KRLLKDIPLENCTKEDSFLRDIYCPDGKCEEYYLANNVTKVKGIKGLASG
VFYDNIFPSFLEKGQFISYGKSAIDIENTSGESYNQIMADITTSFTLLIG
IFFPSVTGIMAGSNRSGDLADAQKSIPIGTICAILTTSTVYLSSVMFFAG
TVDNLLLRDKFGQSIGGKLVVANIAWPNQWVILIGSFLSTLGAGLQSLTG
APRLLQAIARDEIIPFLAPFAKSSKRGEPTRALLLTIVICQCGILLGNVD
LLAPLLSMFFLMCYGFVNLACAVQTLLRTPNWRPRFKFYHWSLSLIGLTL
CISVMIMTSWYFALIAMGMAIIIYKYIEYRGAEKEWGDGIRGMALTAARY
SLLRLEEGPPHTKNWRPQILVLSKLNDNLLPKYRKIFSFATQLKAGKGLT
ICVSVIKGDHTKITNKAVDAKATLRKYMTDEKVKGFCDVLVAQQIGEGLS
SVIQTIGLGGMKPNTVIIGWPYSWRQEGRNSWKTFIQTVRTVAACHMALM
VPKGINFYPESNHKIGGNIDIWWIVHDGGLLMLLPFLLKQHRTWRNCKLR
IFTVAQIEDNSIQMKKDLKTFLYHLRIEADVEVVEMNNSDISAYTYERTL
MMEQRNQMLRALGLNKKENSKVVQTIVDHHYDATKTASKVRFADPTIEET
QHHDSQNDEKRNSIDLDGPENADTPETTSNKDESTEKADGDFKSSVKPDE
FNVRRMHTAIKLNEVIVEKSQDAQLVIMNLPGPPREVRAERERNYMEFLE
VLTEGLEKVLMVRGGGREVITIYSDYKDDDDK*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48988
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform D, contains an extra exon as compared to the main variant A. This exon is 31 residues in length and appears between C922 and D923.; Several silent changes as compared to the reference sequence; G>C 1527, G>A1596, A>T 2058, T>C 2235, A>G 2325, T>C 2451 and T>C 2523 bp.