cDNA Clone KLHL3

• Synonyms: PHA2D
• Plasmid: pBABED P U6 KLHL3 Cter KI sense
• Parent Plasmid: pBABED P U6
• Expressed: KLHL3 Cter KI sense guide
• Genbank: ENSG00000146021
• Species: Human

Sequence of Insert

gCCCAAACTCCTACTGCCAGG

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48975
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Targets end of coding region, designed to KI a c-terminal GFP reporter to KLHL3 when co-transfected with antisense guide 48979 and donor 52069. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty flp-in line is preferable as this allows for downstream rescue and mutant analysis if necessary.