Synonyms
PHA2D
Plasmid
pX335 KLHL3 Cter KI antisense
Parent Plasmid
pX335
Expressed
KLHL3 Cter KI antisense guide + Cas9 D10A
Genbank
ENSG00000146021
Species
Human
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU48979
Unit Source
Location
PPU
MTA
Notes
CRISPR guide plasmid. Targets end of coding region, designed to KI a c-terminal GFP reporter to KLHL3 when co-transfected with sense guide 48975 and donor 52069. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty flp-in line is preferable as this allows for downstream rescue and mutant analysis if necessary.
Price
£125.00
To submit an order for this reagent acceptance of the Dundee Material Transfer Agreement is required at checkout.