cDNA Clone CLASP1

• Synonyms: MAST1
• Plasmid: pCMV5 FLAG2 CLASP1 tv3 R953A
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG-CLASP1 tv3 R953A
• Genbank: NM_001142274.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCAATGGAGCCTCGCATGGAGTCCTGCCTGGCGCAGGTGTTGCAGAAGGATGTGGGGAAACGATTGCAGGTTGGCCAAGAACTGATAGACTATTTCTCAGACAAACAGAAGTCTGCTGACCTTGAGCATGACCAGACCATGTTAGATAAACTTGTGGATGGACTTGCTACCTCTTGGGTGAACTCTAGCAATTACAAGGTGGTTCTGCTGGGCATGGACATCCTGTCCGCCCTGGTGACCCGGCTGCAGGATCGGTTCAAGGCGCAGATCGGCACAGTGCTGCCAAGTCTAATAGACAGACTAGGAGATGCTAAAGACTCTGTGAGGGAGCAGGACCAAACTCTGCTGCTAAAGATCATGGATCAAGCTGCTAATCCCCAGTACGTATGGGACAGAATGCTTGGAGGCTTCAAACACAAGAATTTCCGTACTCGAGAAGGCATCTGTCTCTGCCTTATAGCAACACTCAATGCCTCTGGAGCACAGACTTTAACACTAAGCAAGATTGTGCCACATATATGCAACTTACTTGGAGATCCAAACAGCCAGGTTCGAGATGCAGCAATAAACAGCTTAGTGGAAATTTACAGACATGTAGGAGAACGTGTGAGGGCAGATCTCAGTAAAAAAGGATTGCCACAGTCCCGGTTGAATGTAATTTTTACAAAATTTGATGAAGTCCAGAAATCTGGAAACATGATACAATCTGCAAATGATAAAAATTTCGACGATGAAGATTCTGTGGATGGTAACAGACCTTCCTCTGCTAGTTCTACATCATCCAAGGCTCCACCAAGTTCTCGGAGAAACGTTGGAATGGGAACCACCCGCCGGCTTGGTTCATCCACCCTTGGATCCAAGTCTTCAGCTGCAAAAGAAGGAGCTGGTGCTGTTGATGAAGAGGATTTTATTAAAGCATTTGATGATGTACCTGTAGTACAGATTTATTCCAGCCGAGACCTTGAGGAATCCATAAACAAAATTAGGGAAATATTATCTGATGACAAGCATGATTGGGAGCAGAGAGTAAATGCTCTAAAAAAGATTAGATCTTTACTTTTGGCTGGTGCTGCTGAGTATGATAACTTCTTTCAACATTTGCGTCTTTTGGATGGAGCCTTTAAACTCTCTGCTAAGGACCTGCGGTCTCAGGTAGTGCGGGAGGCTTGTATCACGTTGGGGCATCTGTCATCAGTTCTGGGGAATAAGTTTGACCATGGAGCTGAAGCCATTATGCCAACTATCTTTAATTTAATTCCAAACAGTGCCAAAATTATGGCCACATCTGGTGTTGTAGCTGTTAGGTTAATTATTCGGCACACACACATCCCTAGGTTAATACCTGTCATAACAAGCAACTGTACCTCTAAGTCTGTCGCAGTTAGAAGGCGCTGTTTTGAATTTTTAGATTTGCTTTTACAAGAATGGCAGACACATTCACTAGAACGACACATATCAGTATTAGCTGAAACAATAAAGAAGGGAATACATGATGCTGATTCCGAAGCAAGAATAGAAGCCAGAAAATGTTACTGGGGTTTCCACAGTCACTTCAGCAGAGAAGCAGAGCACTTGTACCACACCTTGGAGTCCTCCTACCAGAAAGCCCTGCAGTCCCACCTGAAGAACTCAGACAGCATAGTGTCTCTGCCTCAGTCAGACCGCTCATCTTCCAGCTCTCAAGAGAGTCTAAATCGTCCGCTGTCTGCCAAAAGAAGTCCTACTGGAAGTACCACATCTAGAGCTTCTACAGTTAGTACCAAATCTGTGTCAACGACTGGGTCCCTCCAGCGATCTCGAAGTGATATTGATGTGAACGCAGCAGCCAGTGCCAAATCCAAAGTCTCCTCATCTTCGGGCACGACGCCTTTCAGCTCTGCAGCAGCTTTGCCTCCAGGGTCATACGCATCCTTAGGTCGGATCCGCACAAGACGGCAAAGCTCTGGGAGTGCCACCAACGTCGCCTCTACACCTGATAACCGGGGCCGCAGTCGCGCTAAAGTGGTTTCACAGTCCCAGCGATCCAGATCTGCTAATCCTGCTGGTGCTGGCAGCCGGTCAAGTTCCCCAGGAAAATTGTTGGGAAGTGGTTATGGTGGACTTACTGGGGGCTCCTCACGAGGCCCACCTGTGACACCGTCTTCAGAAAAGCGAAGCAAGATTCCCAGGAGCCAGGGATGTAGCCGGGAAACAAGTCCAAACCGAATAGGATTAGATCGGTTTGGGCTTGGCCAGCCAGGAAGAATACCTGGTTCTGTGAATGCCATGAGAGTTCTGAGCACAAGTACAGATCTTGAAGCTGCTGTTGCTGATGCTTTGCTGTTAGGAGACTCCAGGAGCAAGAAGAAGCCTGTGAGGAGGAGATATGAGCCGTATGGGATGTATTCTGACGATGATGCCAACAGTGATGCCTCAAGTGTTTGCTCTGAGCGCTCATATGGCTCCAGGAATGGTGGCATTCCCCATTATCTGCGGCAGACTGAGGATGTAGCAGAAGTTCTCAACCACTGTGCTAGTTCAAACTGGTCAGAAAGGAAAGAAGGGCTTCTGGGCCTGCAGAACTTACTGAAGAGCCAAAGAACACTGAGTCGAGTTGAACTGAAAAGGTTGTGTGAGATCTTCACTCGGATGTTTGCTGACCCTCATAGCAAGAGAGTTTTCAGTATGTTTTTGGAGACTCTTGTGGATTTTATAATAATTCATAAGGATGATTTACAAGACTGGCTTTTTGTTCTTCTCACACAATTACTTAAGAAAATGGGAGCAGATTTACTTGGATCTGTGCAAGCAAAAGTTCAAAAGGCTCTAGATGTCACAAGGGACTCCTTTCCATTTGATCAACAATTTAACATTTTGATGGCATTTATTGTGGATCAAACTCAAACTCCAAACCTCAAGGTCAAAGTTGCAATCCTGAAATACATTGAGTCTCTGGCCAGACAGATGGATCCAACAGATTTTGTAAACTCTAGTGAGACAAGGCTTGCTGTTTCTAGAATCATAACCTGGACAACAGAACCAAAGAGTTCAGACGTGAGAAAGGCAGCACAGATTGTGCTAATCTCTCTGTTTGAATTGAATACTCCTGAATTTACCATGTTACTTGGTGCCTTGCCAAAAACATTCCAGGATGGTGCCACCAAACTCCTGCACAACCACCTCAAGAATTCCAGTAACACCAGTGTGGGCTCTCCAAGCAATACGATTGGCCGGACGCCCTCCCGACACACCAGCAGCAGGACCAGCCCCCTGACCTCACCCACCAACTGTTCCCATGGGGGTCTGTCTCCAAGCATGCTGGACTATGATACAGAGAACCTGAACTCTGAAGAAATCTATAGTTCTCTACGTGGAGTTACAGAAGCCATTGAAAAGTTTAGTTTTCGAAGCCAAGAAGATCTGAATGAGCCAATTAAACGAGATGGCAAAAAGGAGTGTGATATTGTGTCCCGCGATGGGGGCGCTGCCTCCCCTGCCACTGAGGGCCGGGGGGGTAGTGAAGTAGAAGGAGGCCGGACAGCTCTGGATAACAAGACCTCACTACTCAACACCCAGCCTCCGCGCGCCTTCCCGGGGCCGCGGGCGCGAGACTACAACCCGTACCCCTACTCAGATGCCATCAACACCTACGACAAGACCGCCCTGAAAGAGGCTGTGTTCGATGACGACATGGAGCAGCTTCGAGACGTGCCCATCGACCATTCTGACCTGGTGGCTGACCTTCTGAAAGAGCTGTCCAACCACAATGAGCGAGTGGAGGAACGGAAGGGAGCCCTGCTGGAGCTGCTCAAGATCACGCGGGAAGACAGCCTTGGTGTCTGGGAGGAGCACTTCAAGACCATTCTGCTCCTGCTGCTGGAGACCCTTGGAGACAAAGACCATTCAATTCGAGCACTGGCGTTAAGAGTTTTGAGGGAAATTCTGAGAAATCAACCAGCAAGATTTAAAAACTACGCCGAGCTGACGATTATGAAGACTCTGGAAGCCCACAAAGACTCCCATAAGGAGGTGGTGAGAGCGGCTGAGGAGGCTGCGTCCACACTGGCCAGTTCCATCCACCCGGAGCAGTGCATCAAGGTGCTCTGCCCCATCATCCAGACGGCCGACTACCCCATCAACCTTGCTGCCATCAAGATGCAGACCAAAGTCGTCGAGAGGATCGCAAAGGAGTCATTGCTGCAGCTCCTTGTCGACATCATCCCAGGCTTGCTGCAGGGTTATGACAACACCGAAAGTAGTGTGCGTAAGGCCAGCGTGTTTTGCTTAGTGGCAATTTATTCCGTAATCGGAGAAGACCTGAAACCTCACCTTGCACAGCTCACAGGGAGCAAGATGAAGCTACTAAACTTATACATAAAGAGGGCCCAGACCACCAACAGCAACAGCAGCTCCTCCTCCGATGTCTCCACGCACAGCTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDKQKSADLEHDQTMLDKLVDGLATSWVNSSNYKVVLLGMDILSALV
TRLQDRFKAQIGTVLPSLIDRLGDAKDSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKNFRTREGICLCLIATLNASGAQTLTLSKIVPHICNLLGD
PNSQVRDAAINSLVEIYRHVGERVRADLSKKGLPQSRLNVIFTKFDEVQKSGNMIQSANDKNFDDEDSVDGNRPSSASSTSSKAPPSSRRNVGMGTTRRL
GSSTLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIREILSDDKHDWEQRVNALKKIRSLLLAGAAEYDNFFQHLRLLDGAFKLSAK
DLRSQVVREACITLGHLSSVLGNKFDHGAEAIMPTIFNLIPNSAKIMATSGVVAVRLIIRHTHIPRLIPVITSNCTSKSVAVRRRCFEFLDLLLQEWQTH
SLERHISVLAETIKKGIHDADSEARIEARKCYWGFHSHFSREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQESLNRPLSAKRSPTGSTT
SRASTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGTTPFSSAAALPPGSYASLGRIRTRRQSSGSATNVASTPDNRGRSRAKVVSQSQRSRSAN
PAGAGSRSSSPGKLLGSGYGGLTGGSSRGPPVTPSSEKRSKIPRSQGCSRETSPNRIGLDRFGLGQPGRIPGSVNAMRVLSTSTDLEAAVADALLLGDSR
SKKKPVRRRYEPYGMYSDDDANSDASSVCSERSYGSRNGGIPHYLRQTEDVAEVLNHCASSNWSERKEGLLGLQNLLKSQRTLSRVELKRLCEIFTRMFA
DPHSKRVFSMFLETLVDFIIIHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDVTRDSFPFDQQFNILMAFIVDQTQTPNLKVKVAILKYIESLA
RQMDPTDFVNSSETRLAVSRIITWTTEPKSSDVRKAAQIVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLKNSSNTSVGSPSNTIGRTPSRHTSS
RTSPLTSPTNCSHGGLSPSMLDYDTENLNSEEIYSSLRGVTEAIEKFSFRSQEDLNEPIKRDGKKECDIVSRDGGAASPATEGRGGSEVEGGRTALDNKT
SLLNTQPPRAFPGPRARDYNPYPYSDAINTYDKTALKEAVFDDDMEQLRDVPIDHSDLVADLLKELSNHNERVEERKGALLELLKITREDSLGVWEEHFK
TILLLLLETLGDKDHSIRALALRVLREILRNQPARFKNYAELTIMKTLEAHKDSHKEVVRAAEEAASTLASSIHPEQCIKVLCPIIQTADYPINLAAIKM
QTKVVERIAKESLLQLLVDIIPGLLQGYDNTESSVRKASVFCLVAIYSVIGEDLKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSSSDVSTHS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU52098
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: R953A. Variant 3.