cDNA Clone Jerky

• Plasmid: pcDNA5 FRT/TO GFP Jerky
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP Jerky
• Genbank: AAK34940.1 GI:13625162 with residue changes T207K and S323T (RNA-derived)
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGAATAAGAGAGGGAAATATACAACACTGAATTTGGAGGAGAAAATGAAGGTTCTAAGTAGAATTGAAGCTGGACGATCACTTAAAAGTGTAATGGATGAATTTGGAATCAGTAAGTCAACATTTTATGACATTAAAAAAAATAAGAAGTTAATTCTGGACTTTGTACTGAAGCAGGACATGCCATTAGTAGGGGCTGAGAAGAGAAAGAGGACAACGGGAGCCAAATATGGTGATGTAGATGATGCGGTCTACATGTGGTACCAACAGAAACGCTCAGCCGGTGTTCCGGTAAGAGGCGTGGAGCTTCAGGCTGCTGCAGAGAGATTTGCACGGTGTTTTGGGCGAACAGATTTCAAAGCTAGCACTGGTTGGCTTTTTAGATTTCGAAATCGGCATGCAATTGGGAACCGAAAAGGATGTGGGGAACAAGTCCTAAGTTCAGTTTCTGAAAATGTTGAGCCATTTCGACAAAAACTGTCCATGATAATCAAAGAGGAGAAACTGTGTCTAGCTCAGCTATACAGTGGGGATGAAACAGACCTCTTTTGGAAGTCAATGCCAGAAAATTCTCAGGCAAGTAGGAAAGATATCTGCCTACCAGGGAAGAAAATAAACAAAGAAAGGTTGTCTGCCTTTTTATGTGCAAATGCAGACGGAACTCATAAATTAAAGTCAATCATTATTGGAAAATCAAAACTGCCCAAAAGTGTGAAAGAGGACACAAGTACATTGCCTGTGATATATAAACCCAGTAAAGATGTTTGGTTCACCAGAGAATTGTTTTCAGAATGGTTTTTTCAAAACTTTGTTCCTGAGGTCCGACATTTTCAACTTAATGTTCTAAGATTTCATGACGAGGACGTCAGGGCATTGTTACTTCTGGACAGTTGCCCGGCTCATCCTTCCTCTGAATCCCTAACCAGTGAGGATGGTCGAATAAAATGTATGTTCTTCCCCCATAACACTTCAACCTTGATTCAACCAATGAATCAAGGTGTGATCTTGAGCTGCAAACGGCTGTATAGATGGAAGCAACTTGAAGAGAGTCTTGTAATATTTGAAGAAAGTGATGATGAGCAAGAGAAAGGAGATAAAGGAGTTTCCAAAATTAAAATCTACAATATAAAAAGTGCAATTTTTAACTGGGCAAAAAGTTGGGAAGAAGTAAAACAAATAACCATAGCAAATGCATGGGAAAATCTTCTTTACAAAAAGGAACCTGAATATGATTTTCAAGGCTTAGAACATGGGGATTATAGAGAAATTCTTGAAAAATGTGGGGAGTTGGAAACTAAGTTGGATGATGATAGGGTGTGGTTAAATGGAGATGAAGAAAAGGGTTGTCTCCTAAAAACCAAAGGTGGAATAACAAAGGAAGTTGTTCAAAAAGGAGGAGAAGCTGAGAAGCAGACTGCTGAATTTAAATTATCTGCTGTAAGAGAGAGTTTGGACTACCTTCTTGACTTTGTTGATGCCACACCTGAGTTTCAGAGATTCCACTTCACACTGTGTGAGTTCAGTGATGACTCATAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMNKRGKYTTLNLEEKMKVLSRIEAGRSLKSVMDEFGISKSTFYDIKKNKKLILDFV
LKQDMPLVGAEKRKRTTGAKYGDVDDAVYMWYQQKRSAGVPVRGVELQAAAERFARCFGRTDFKASTGWLFRFRNRHAIGNRKGCGEQVLSSVSENVEPF
RQKLSMIIKEEKLCLAQLYSGDETDLFWKSMPENSQASRKDICLPGKKINKERLSAFLCANADGTHKLKSIIIGKSKLPKSVKEDTSTLPVIYKPSKDVW
FTRELFSEWFFQNFVPEVRHFQLNVLRFHDEDVRALLLLDSCPAHPSSESLTSEDGRIKCMFFPHNTSTLIQPMNQGVILSCKRLYRWKQLEESLVIFEE
SDDEQEKGDKGVSKIKIYNIKSAIFNWAKSWEEVKQITIANAWENLLYKKEPEYDFQGLEHGDYREILEKCGELETKLDDDRVWLNGDEEKGCLLKTKGG
ITKEVVQKGGEAEKQTAEFKLSAVRESLDYLLDFVDATPEFQRFHFTLCEFSDDS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50459
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee