cDNA Clone Cul2 (xenopus)

• Synonyms: Cullin2
• Plasmid: pET6HIS SUMO CUL2 1-277 (xenopus)
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6His SUMO Xenopus Cul2 1-277
• Tag: SUMO
• Tag: 6His
• Species: Xenopus laevis

Sequence of Insert

ATGTCATTGAAGCCACGGGTAGTGGATTTTGATGAAACATGGAACAAACTTTTAACTACAATCAAAGCTGTTGTCATGCTGGATTATGTTGAAAGAGCAA
CATGGAATGACCGTTTCTCAGATATTTACGCACTGTGTGTTGCCTATCCAGAGCCTCTTGGAGAAAGATTGTATACAGAAACAAAGATATTTCTGGAAAA
CCATGTACAGCAGTTGCATACGAGAGTTCTGGATTCAGCAGAGCAAGTACTGGTAATGTACTTCAGATACTGGGAGGAATATAGCAGAGGAGCTGATTAC
ATGGATTGTTTATATAGGTATCTTAACACACAGTACATTAAAAAGAACAAGCTGACAGAAGCAGACCTGCAGTATGGCTATGGTGGAGTAGATATGAATG
AACCTCTGATGGAGATAGGAGAATTAGCACTGGATTTGTGGAGGAAATTAATGACAGAGCCTCTCCAAGACACGCTCCTCATAATGCTTCTCCGAGAAAT
TAAAAGGGATCGATGTGGGGAAGACCCAAATCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTTCCCTTA
AAGTTTTATCAAGAAATATTTGAGTCGCCTTTTCTGGCTGAAACAGGGGAGTATTATAAACAAGAAGCTTCAAATTTATTACAAGAGTCAAACTGCTCAC
AATATATGGAAAAGATTTTAGGTAGATTAAAAGATGAAGAAATCAGATGTCGGAAGTATTTACACCCCAGCTCATATAATAAAGTGATTCACGAATGCCA
GCAGAGAATGGTGGCAGATCACTTGCAGTTTTAACATGCGGAATGTCACAATATCATCAGACAAGAAAGGAGAAACGATATGGCCAACATGTATACTCTT
CTTCGTGCTGTATCCAGTGGTTTACCTCATATGATTCAGGAACTCCAAAACCATATCCACGATGAAGGCCTTAGAGCTATTAGTAATCTTTCTCAGGAAA
ACATGCCAACTCAATTTCGGGAGTCAGTTGATGAAGTTCACAGTAAATTTGTTCAGCTTGTCAACTGTGTATTGAATGGTGATCAGCATTTTATGAGTGC
ACTAGACAAGGCTCTTACTTGTGTGGTGAACTACAGGGAACCAAAATCAtgaGCGGCCGC

Amino Acid Sequence

MSLKPRVVDFDETWNKLLTTIKAVVMLDYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVQQLHTRVLDSAEQVLVMYFRYWEEYSRGADY
MDCLYRYLNTQYIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDLWRKLMTEPLQDTLLIMLLREIKRDRCGEDPNQKVIHGVINSFVHVEQYKKKFPL
KFYQEIFESPFLAETGEYYKQEASNLLQESNCSQYMEKILGRLKDEEIRCRKYLHPSSYNKVIHECQQRMVADHLQF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51229
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you add 6His-SUMO (and no other amino acids) at the N-terminus of a fragment of Xenopus laevis Cul2, comprising amino acids 1-277. We already have a vector with the Xenopus Cul2 cDNA sequence. I want to make soluble protein and also generate antibodies.