cDNA Clone Ctf4

• Plasmid: pGAD CTF4 (yeast) 1-927 L867E A871E A897E I901E
• Parent Plasmid: pGAD
• Expressed: GalAD-Ctf4(1-927) L867E A871E A897E I901E
• Genbank: NP_015461.1
• Tag: GAL4 DNA-AD
• Species: Yeast

Sequence of Insert

ATGGTTTCAGTTATAGACAAGCTTGTTTTTGACTTTGGTGGGAAAACTCTGGTCTCTCTCGCACCAGATAATAATACTTTGTGTGTAGCCAATAAAAATG
GCTTAACCAAGATTCTGAAGACAAATAACCCAGAAGAAGAGCCAGAGACTTTAGATTCTTCCAAATTGGTCTCGTCTATAAAATGCTATTCAAACTCGCA
CTTTCTGATGACTACAATGCAAGGTGATGCTCTTAGGTATAACATCGATTCTAGTCAAGAAGAATTATTGGCTAGATTTGCTTTACCCCTACGTGACTGC
TGTGTTATTCATTCAGGTAAAATGGCCGTATTTGGAGGAGACGACTTAGAACTAATCCTTTTGGAATTGGACGATGAAACACACAAAAAACATGCCATTA
AAATCGATGAACAAGTTTCTCAAATTTCTTACAATTCACAGATGAATATTTTAGCAGTTTCAATGATAAATGGTAAGGTACAAATTTTTTCTCTGACATC
TACTATTCCAAACAAAGTTCATGAGTTAAATGATTACATAGTGGCCAATTCATATGATGATACACACAGAGATAAGATACTCTCGAATATGATGGATGAT
ATAGATAAAGACAATGATAATGACCTGAGTGAAACGGCTGATCCAGATGAGAACAATGTAGCTGATCCAGAATTCTGTGCTGCTAATAGAATTTGCACGA
GAGTGGCTTGGCATCCGAAGGGTCTGCATTTTGCGCTACCATGTGCAGATGATACAGTAAAAATATTCTCTATAAAGGGATATTCCCTACAAAAGACGTT
GTCCACAAATCTCTCATCAACAAAGGCTCATTTCATTGATTTGCAATTTGACCCGTTACGTGGAACTTACATTGCGGCAGTAGATTTAAATAATAAGTTA
ACGGTATGGAATTGGGAAACTTCCGAGATCCACTACACCAGGGAATTCAAAAGAAAAATTACTAATATTGCCTGGAAAATCCAAGCGGACTCAAAAACTC
TAGACCTTGTTTTAGGTACCTGGTCCGGTAGTATAGCCATTGTCCAAAATTTGGCAGAGTCCGTAGTATCTAATATACCTGACCAATCTGTTGCTGAATC
TTCAACTAAACATGGGCTTTTTGTAGACTCCGAATCTGACTTGGAAAACTTAGAGGGAAATGATGATATAAACAAAAGCGATAAGCTATTTTCAGATATT
ACTCAAGAAGCGAATGCGGAAGATGTGTTCACTCAAACACACGACGGCCCCAGTGGATTAAGTGAAAAGAGAAAATACAACTTCGAAGATGAAGAAGACT
TTATTGATGATGACGATGGTGCTGGTTATATTAGTGGCAAAAAACCACATAATGAACATTCTTATTCGAGAGTACACAAGACTCATTCGTTTCCAATCAG
TTTGGCAAACACAGGAAAATTTCGTTATATGCCTTTTTCTCCAGCGGGAACACCTTTTGGCTTTACTGACAGGCGTTATTTGACAATGAATGAAGTGGGC
TACGTATCTACTGTCAAGAATAGTGAGCAATACAGCATAACTGTCTCTTTTTTTGATGTTGGACGTTTTAGAGAATACCATTTCGAGGACTTATTTGGCT
ACGATTTATGCTTCCTAAATGAAAAAGGCACTTTATTTGGCCAATCCAAAACTGGGCAGATACAATATAGGCCACACGATAGCATACATTCAAACTGGAC
CAAGATTATTCCTTTGCAAGCTGGTGAGAGAATAACAAGTGTGGCAGCCACCCCGGTTCGCGTTATTGTTGGTACATCATTAGGCTATTTCAGAAGTTTC
AATCAATTCGGGGTTCCATTTGCTGTTGAAAAGACATCCCCAATTGTAGCGCTTACTGCTCAGAATTATAGGGTTTTTTCAGTACATTATTCGCAGTTTC
ATGGCCTTTCATACTCTTTATCTGAATTGGGTACTTCTAGTAAAAGGTACTATAAAAGAGAGTGTCCACTTCCAATGAGTTTACCAAACATTAATTCTGA
TATGAAAAAAGACGCAAATCTTGACTACTACAATTTTAATCCGATGGGCATCAAAAGTTTGTTCTTTTCAAGCTACGGAGATCCATGCATTTTTGGGTCC
GACAACACGCTTCTATTGTTATCAAAGTGGAGATCACCAGAAGAAAGTAAATGGCTCCCTATTCTAGATAGCAACATGGAAATATGGAAGATGTCAGGAG
GGAAGGAAACGACAGATATACATGTCTGGCCCTTGGCTTTGGCGTATGACACATTGAATTGTATCTTAGTTAAGGGCAAGCATATATGGCCCGAGTTTCC
CCTTCCGTTGCCATCCGAAATGGAGATTAGAATGCCAGTATTTGTTAAGAGTAAATTACTAGAGGAAAACAAAGCTATATTAAATAAAAAGAACGAAATT
GGAGCTGACACCGAAGCGGAAGAAGGGGAAGAAGACAAAGAAATACAGATTCCTGTTTCTATGGCGGCGGAAGAAGAGTATCTGCGCAGCAAGGTTTTGT
CAGAGCTGTTGACAGATACACTCGAAAATGACGGTGAAATGTACGGCAACGAAAATGAGGTATTGGCAGCATTGAACGGTGCATATGATAAGGCTTTGGA
ACGTTTATTTGAGTCTGCATGCTCAGACCAAAATGTTGAAAAGGCTCTTTCGCTTGCTCATGAATTAAAACAAGATAGAGCACTTACTGAAGCCGTTAAG
GAATCAGAAAGAGCTGAGCTGCCCTCTCTCGTTAAAAAAATTAATAATATAAGGGAAGCTAGATATGAACAGCAATTGAAATAACTCGAG

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAAMEYPYDVPDYAHMAMEAREFHPGGAGAGAGAGAMVSVIDKLVFDFGGKTLVS
LAPDNNTLCVANKNGLTKILKTNNPEEEPETLDSSKLVSSIKCYSNSHFLMTTMQGDALRYNIDSSQEELLARFALPLRDCCVIHSGKMAVFGGDDLELI
LLELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKVQIFSLTSTIPNKVHELNDYIVANSYDDTHRDKILSNMMDDIDKDNDNDLSETADPDENN
VADPEFCAANRICTRVAWHPKGLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKAHFIDLQFDPLRGTYIAAVDLNNKLTVWNWETSEIHYTREFKRK
ITNIAWKIQADSKTLDLVLGTWSGSIAIVQNLAESVVSNIPDQSVAESSTKHGLFVDSESDLENLEGNDDINKSDKLFSDITQEANAEDVFTQTHDGPSG
LSEKRKYNFEDEEDFIDDDDGAGYISGKKPHNEHSYSRVHKTHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSITVSFFD
VGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSFNQFGVPFAVEKTSPIVALT
AQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPEESKWL
PILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKNEIGADTEAEEGEEDKEIQIPV
SMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALERLFESACSDQNVEKALSLAHELKQDRALTEAVKESERAELPSLVKKINNIRE
ARYEQQLK*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU51286
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: I would like a plasmid carrying the full-lenght Ctf4 (1-927) mutated in four sites (L867E, A871E, I901E and A897E). I will provide the initial plasmid (amp resistance) and a DNA strider file with the exact sequence for the final clone.; many thanks; Fabrizio