cDNA Clone DNA mismatch repair protein Msh6 (MSH6)

• Plasmid: pcDNA5D-FRT/TO GFP MSH6
• Parent Plasmid: pcDNA5D-FRT/TO GFP
• Expressed: GFP MSH6
• Genbank: NM_000179.2
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGTCGCGACAGAGCACCCTGTACAGCTTCTTCCCCAAGTCTCC
GGCGCTGAGTGATGCCAACAAGGCCTCGGCCAGGGCCTCACGCGAAGGCG
GCCGTGCCGCCGCTGCCCCCGGGGCCTCTCCTTCCCCAGGCGGGGATGCG
GCCTGGAGCGAGGCTGGGCCTGGGCCCAGGCCCTTGGCGCGATCCGCGTC
ACCGCCCAAGGCGAAGAACCTCAACGGAGGGCTGCGGAGATCGGTAGCGC
CTGCTGCCCCCACCAGTTGTGACTTCTCACCGGGAGATTTGGTTTGGGCC
AAGATGGAGGGTTACCCCTGGTGGCCTTGTCTGGTTTACAACCACCCCTT
TGATGGAACATTCATCCGCGAGAAAGGGAAATCAGTCCGTGTTCATGTAC
AGTTTTTTGATGACAGCCCAACAAGGGGCTGGGTTAGCAAAAGGCTTTTA
AAGCCATATACAGGTTCAAAATCAAAGGAAGCCCAGAAGGGAGGTCATTT
TTACAGTGCAAAGCCTGAAATACTGAGAGCAATGCAACGTGCAGACGAAG
CCTTAAATAAAGACAAGATTAAGAGGCTTGAATTGGCAGTTTGTGATGAG
CCCTCAGAGCCAGAAGAGGAAGAAGAGATGGAGGTAGGCACAACTTACGT
AACAGATAAGAGTGAAGAAGATAATGAAATTGAGAGTGAAGAGGAAGTAC
AGCCTAAGACACAAGGATCTAGGCGAAGTAGCCGCCAAATAAAAAAACGA
AGGGTCATATCAGATTCTGAGAGTGACATTGGTGGCTCTGATGTGGAATT
TAAGCCAGACACTAAGGAGGAAGGAAGCAGTGATGAAATAAGCAGTGGAG
TGGGGGATAGTGAGAGTGAAGGCCTGAACAGCCCTGTCAAAGTTGCTCGA
AAGCGGAAGAGAATGGTGACTGGAAATGGCTCTCTTAAAAGGAAAAGCTC
TAGGAAGGAAACGCCCTCAGCCACCAAACAAGCAACTAGCATTTCATCAG
AAACCAAGAATACTTTGAGAGCTTTCTCTGCCCCTCAAAATTCTGAATCC
CAAGCCCACGTTAGTGGAGGTGGTGATGACAGTAGTCGCCCTACTGTTTG
GTATCATGAAACTTTAGAATGGCTTAAGGAGGAAAAGAGAAGAGATGAGC
ACAGGAGGAGGCCTGATCACCCCGATTTTGATGCATCTACACTCTATGTG
CCTGAGGATTTCCTCAATTCTTGTACTCCTGGGATGAGGAAGTGGTGGCA
GATTAAGTCTCAGAACTTTGATCTTGTCATCTGTTACAAGGTGGGGAAAT
TTTATGAGCTGTACCACATGGATGCTCTTATTGGAGTCAGTGAACTGGGG
CTGGTATTCATGAAAGGCAACTGGGCCCATTCTGGCTTTCCTGAAATTGC
ATTTGGCCGTTATTCAGATTCCCTGGTGCAGAAGGGCTATAAAGTAGCAC
GAGTGGAACAGACTGAGACTCCAGAAATGATGGAGGCACGATGTAGAAAG
ATGGCACATATATCCAAGTATGATAGAGTGGTGAGGAGGGAGATCTGTAG
GATCATTACCAAGGGTACACAGACTTACAGTGTGCTGGAAGGTGATCCCT
CTGAGAACTACAGTAAGTATCTTCTTAGCCTCAAAGAAAAAGAGGAAGAT
TCTTCTGGCCATACTCGTGCATATGGTGTGTGCTTTGTTGATACTTCACT
GGGAAAGTTTTTCATAGGTCAGTTTTCAGATGATCGCCATTGTTCGAGAT
TTAGGACTCTAGTGGCACACTATCCCCCAGTACAAGTTTTATTTGAAAAA
GGAAATCTCTCAAAGGAAACTAAAACAATTCTAAAGAGTTCATTGTCCTG
TTCTCTTCAGGAAGGTCTGATACCCGGCTCCCAGTTTTGGGATGCATCCA
AAACTTTGAGAACTCTCCTTGAGGAAGAATATTTTAGGGAAAAGCTAAGT
GATGGCATTGGGGTGATGTTACCCCAGGTGCTTAAAGGTATGACTTCAGA
GTCTGATTCCATTGGGTTGACACCAGGAGAGAAAAGTGAATTGGCCCTCT
CTGCTCTAGGTGGTTGTGTCTTCTACCTCAAAAAATGCCTTATTGATCAG
GAGCTTTTATCAATGGCTAATTTTGAAGAATATATTCCCTTGGATTCTGA
CACAGTCAGCACTACAAGATCTGGTGCTATCTTCACCAAAGCCTATCAAC
GAATGGTGCTAGATGCAGTGACATTAAACAACTTGGAGATTTTTCTGAAT
GGAACAAATGGTTCTACTGAAGGAACCCTACTAGAGAGGGTTGATACTTG
CCATACTCCTTTTGGTAAGCGGCTCCTAAAGCAATGGCTTTGTGCCCCAC
TCTGTAACCATTATGCTATTAATGATCGTCTAGATGCCATAGAAGACCTC
ATGGTTGTGCCTGACAAAATCTCCGAAGTTGTAGAGCTTCTAAAGAAGCT
TCCAGATCTTGAGAGGCTACTCAGTAAAATTCATAATGTTGGGTCTCCCC
TGAAGAGTCAGAACCACCCAGACAGCAGGGCTATAATGTATGAAGAAACT
ACATACAGCAAGAAGAAGATTATTGATTTTCTTTCTGCTCTGGAAGGATT
CAAAGTAATGTGTAAAATTATAGGGATCATGGAAGAAGTTGCTGATGGTT
TTAAGTCTAAAATCCTTAAGCAGGTCATCTCTCTGCAGACAAAAAATCCT
GAAGGTCGTTTTCCTGATTTGACTGTAGAATTGAACCGATGGGATACAGC
CTTTGACCATGAAAAGGCTCGAAAGACTGGACTTATTACTCCCAAAGCAG
GCTTTGACTCTGATTATGACCAAGCTCTTGCTGACATAAGAGAAAATGAA
CAGAGCCTCCTGGAATACCTAGAGAAACAGCGCAACAGAATTGGCTGTAG
GACCATAGTCTATTGGGGGATTGGTAGGAACCGTTACCAGCTGGAAATTC
CTGAGAATTTCACCACTCGCAATTTGCCAGAAGAATACGAGTTGAAATCT
ACCAAGAAGGGCTGTAAACGATACTGGACCAAAACTATTGAAAAGAAGTT
GGCTAATCTCATAAATGCTGAAGAACGGAGGGATGTATCATTGAAGGACT
GCATGCGGCGACTGTTCTATAACTTTGATAAAAATTACAAGGACTGGCAG
TCTGCTGTAGAGTGTATCGCAGTGTTGGATGTTTTACTGTGCCTGGCTAA
CTATAGTCGAGGGGGTGATGGTCCTATGTGTCGCCCAGTAATTCTGTTGC
CGGAAGATACCCCCCCCTTCTTAGAGCTTAAAGGATCACGCCATCCTTGC
ATTACGAAGACTTTTTTTGGAGATGATTTTATTCCTAATGACATTCTAAT
AGGCTGTGAGGAAGAGGAGCAGGAAAATGGCAAAGCCTATTGTGTGCTTG
TTACTGGACCAAATATGGGGGGCAAGTCTACGCTTATGAGACAGGCTGGC
TTATTAGCTGTAATGGCCCAGATGGGTTGTTACGTCCCTGCTGAAGTGTG
CAGGCTCACACCAATTGATAGAGTGTTTACTAGACTTGGTGCCTCAGACA
GAATAATGTCAGGTGAAAGTACATTTTTTGTTGAATTAAGTGAAACTGCC
AGCATACTCATGCATGCAACAGCACATTCTCTGGTGCTTGTGGATGAATT
AGGAAGAGGTACTGCAACATTTGATGGGACGGCAATAGCAAATGCAGTTG
TTAAAGAACTTGCTGAGACTATAAAATGTCGTACATTATTTTCAACTCAC
TACCATTCATTAGTAGAAGATTATTCTCAAAATGTTGCTGTGCGCCTAGG
ACATATGGCATGCATGGTAGAAAATGAATGTGAAGACCCCAGCCAGGAGA
CTATTACGTTCCTCTATAAATTCATTAAGGGAGCTTGTCCTAAAAGCTAT
GGCTTTAATGCAGCAAGGCTTGCTAATCTCCCAGAGGAAGTTATTCAAAA
GGGACATAGAAAAGCAAGAGAATTTGAGAAGATGAATCAGTCACTACGAT
TATTTCGGGAAGTTTGCCTGGCTAGTGAAAGGTCAACTGTAGATGCTGAA
GCTGTCCATAAATTGCTGACTTTGATTAAGGAATTATAGgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMSRQST
LYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPGGDAAWSEAGPG
PRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKMEGYPWW
PCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKPYTGSKS
KEAQKGGHFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPSEPEEEE
EMEVGTTYVTDKSEEDNEIESEEEVQPKTQGSRRSSRQIKKRRVISDSES
DIGGSDVEFKPDTKEEGSSDEISSGVGDSESEGLNSPVKVARKRKRMVTG
NGSLKRKSSRKETPSATKQATSISSETKNTLRAFSAPQNSESQAHVSGGG
DDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFDASTLYVPEDFLNSC
TPGMRKWWQIKSQNFDLVICYKVGKFYELYHMDALIGVSELGLVFMKGNW
AHSGFPEIAFGRYSDSLVQKGYKVARVEQTETPEMMEARCRKMAHISKYD
RVVRREICRIITKGTQTYSVLEGDPSENYSKYLLSLKEKEEDSSGHTRAY
GVCFVDTSLGKFFIGQFSDDRHCSRFRTLVAHYPPVQVLFEKGNLSKETK
TILKSSLSCSLQEGLIPGSQFWDASKTLRTLLEEEYFREKLSDGIGVMLP
QVLKGMTSESDSIGLTPGEKSELALSALGGCVFYLKKCLIDQELLSMANF
EEYIPLDSDTVSTTRSGAIFTKAYQRMVLDAVTLNNLEIFLNGTNGSTEG
TLLERVDTCHTPFGKRLLKQWLCAPLCNHYAINDRLDAIEDLMVVPDKIS
EVVELLKKLPDLERLLSKIHNVGSPLKSQNHPDSRAIMYEETTYSKKKII
DFLSALEGFKVMCKIIGIMEEVADGFKSKILKQVISLQTKNPEGRFPDLT
VELNRWDTAFDHEKARKTGLITPKAGFDSDYDQALADIRENEQSLLEYLE
KQRNRIGCRTIVYWGIGRNRYQLEIPENFTTRNLPEEYELKSTKKGCKRY
WTKTIEKKLANLINAEERRDVSLKDCMRRLFYNFDKNYKDWQSAVECIAV
LDVLLCLANYSRGGDGPMCRPVILLPEDTPPFLELKGSRHPCITKTFFGD
DFIPNDILIGCEEEEQENGKAYCVLVTGPNMGGKSTLMRQAGLLAVMAQM
GCYVPAEVCRLTPIDRVFTRLGASDRIMSGESTFFVELSETASILMHATA
HSLVLVDELGRGTATFDGTAIANAVVKELAETIKCRTLFSTHYHSLVEDY
SQNVAVRLGHMACMVENECEDPSQETITFLYKFIKGACPKSYGFNAARLA
NLPEEVIQKGHRKAREFEKMNQSLRLFREVCLASERSTVDAEAVHKLLTL
IKEL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24897
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: compared to NCBI ref clone has silent mutations at: R62 (CGC>CGA), P92 (CCA>CCG), D180 (GAT>GAC)