cDNA Clone NEDD4L

• Plasmid: pSc-B-NEDD4L E576-D955 C922A
• Parent Plasmid: pSC-B
• Genbank: NP_056092
• Species: Human

Sequence of Insert

GAATTCAAGCAGAAATATGACTACTTCAGGAAGAAATTAAAGAAACCTGC
TGATATCCCCAATAGGTTTGAAATGAAACTTCACAGAAATAACATATTTG
AAGAGTCCTATCGGAGAATTATGTCCGTGAAAAGACCAGATGTCCTAAAA
GCTAGACTGTGGATTGAGTTTGAATCAGAGAAAGGTCTTGACTATGGGGG
TGTGGCCAGAGAATGGTTCTTCTTACTGTCCAAAGAGATGTTCAACCCCT
ACTACGGCCTCTTTGAGTACTCTGCCACGGACAACTACACCCTTCAGATC
AACCCTAATTCAGGCCTCTGTAATGAGGATCATTTGTCCTACTTCACTTT
TATTGGAAGAGTTGCTGGTCTGGCCGTATTTCATGGGAAGCTCTTAGATG
GTTTCTTCATTAGACCATTTTACAAGATGATGTTGGGAAAGCAGATAACC
CTGAATGACATGGAATCTGTGGATAGTGAATATTACAACTCTTTGAAATG
GATCCTGGAGAATGACCCTACTGAGCTGGACCTCATGTTCTGCATAGACG
AAGAAAACTTTGGACAGACATATCAAGTGGATTTGAAGCCCAATGGGTCA
GAAATAATGGTCACAAATGAAAACAAAAGGGAATATATCGACTTAGTCAT
CCAGTGGAGATTTGTGAACAGGGTCCAGAAGCAGATGAACGCCTTCTTGG
AGGGATTCACAGAACTACTTCCTATTGATTTGATTAAAATTTTTGATGAA
AATGAGCTGGAGTTGCTCATGTGCGGCCTCGGTGATGTGGATGTGAATGA
CTGGAGACAGCATTCTATTTACAAGAACGGCTACTGCCCAAACCACCCCG
TCATTCAGTGGTTCTGGAAGGCTGTGCTACTCATGGACGCCGAAAAGCGT
ATCCGGTTACTGCAGTTTGTCACAGGGACATCGCGAGTACCTATGAATGG
ATTTGCCGAACTTTATGGTTCCAATGGTCCTCAGCTGTTTACAATAGAGC
AATGGGGCAGTCCTGAGAAACTGCCCAGAGCTCACACAgcCTTTAATCGC
CTTGACTTACCTCCATATGAAACCTTTGAAGATTTACGAGAGAAACTTCT
CATGGCCGTGGAAAATGCTCAAGGATTTGAAGGGGTGGATTAAGCGGCCG
C

• Vector Type: Bacterial
• Antibiotic: Amp; Kan
• DU Number: DU24916
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: EcoR1 restriction site built into the second residue – silent change at Phe577 (TTT to TTC to incorporate EcoR1 site)