cDNA Clone NEDD4L

• Plasmid: pGEX_TEV-NEDD4L E576-D955 C922A
• Parent Plasmid: pGEX TEV
• Expressed: GST-NEDD4L E576-D955 C922A
• Genbank: NP_056092
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCAAGCAGAAATATGACTACTTCAGGAAGAAATTAAAGAAACCTGC
TGATATCCCCAATAGGTTTGAAATGAAACTTCACAGAAATAACATATTTG
AAGAGTCCTATCGGAGAATTATGTCCGTGAAAAGACCAGATGTCCTAAAA
GCTAGACTGTGGATTGAGTTTGAATCAGAGAAAGGTCTTGACTATGGGGG
TGTGGCCAGAGAATGGTTCTTCTTACTGTCCAAAGAGATGTTCAACCCCT
ACTACGGCCTCTTTGAGTACTCTGCCACGGACAACTACACCCTTCAGATC
AACCCTAATTCAGGCCTCTGTAATGAGGATCATTTGTCCTACTTCACTTT
TATTGGAAGAGTTGCTGGTCTGGCCGTATTTCATGGGAAGCTCTTAGATG
GTTTCTTCATTAGACCATTTTACAAGATGATGTTGGGAAAGCAGATAACC
CTGAATGACATGGAATCTGTGGATAGTGAATATTACAACTCTTTGAAATG
GATCCTGGAGAATGACCCTACTGAGCTGGACCTCATGTTCTGCATAGACG
AAGAAAACTTTGGACAGACATATCAAGTGGATTTGAAGCCCAATGGGTCA
GAAATAATGGTCACAAATGAAAACAAAAGGGAATATATCGACTTAGTCAT
CCAGTGGAGATTTGTGAACAGGGTCCAGAAGCAGATGAACGCCTTCTTGG
AGGGATTCACAGAACTACTTCCTATTGATTTGATTAAAATTTTTGATGAA
AATGAGCTGGAGTTGCTCATGTGCGGCCTCGGTGATGTGGATGTGAATGA
CTGGAGACAGCATTCTATTTACAAGAACGGCTACTGCCCAAACCACCCCG
TCATTCAGTGGTTCTGGAAGGCTGTGCTACTCATGGACGCCGAAAAGCGT
ATCCGGTTACTGCAGTTTGTCACAGGGACATCGCGAGTACCTATGAATGG
ATTTGCCGAACTTTATGGTTCCAATGGTCCTCAGCTGTTTACAATAGAGC
AATGGGGCAGTCCTGAGAAACTGCCCAGAGCTCACACAgcCTTTAATCGC
CTTGACTTACCTCCATATGAAACCTTTGAAGATTTACGAGAGAAACTTCT
CATGGCCGTGGAAAATGCTCAAGGATTTGAAGGGGTGGATTAAGCGGCCG
C

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSPEFKQKYDYFRKKLKKPADIP
NRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAR
EWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGR
VAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILE
NDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWR
FVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQ
HSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAE
LYGSNGPQLFTIEQWGSPEKLPRAHTAFNRLDLPPYETFEDLREKLLMAV
ENAQGFEGVD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24934
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: EcoR1 restriction site built into the second residue – silent change at Phe577 (TTT to TTC to incorporate EcoR1 site)