cDNA Clone FAN1 (MTMR15) (Mouse)

• Plasmid: pBabe.puro.GFP.DU mouse Fan1 (MTMR15) + intron
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP mouse FAN1+ intron
• Genbank: NM_177893.3 a414g silent
• Tag: GFP
• Species: Mouse

Sequence of Insert

GGATCCATGCCGTCACAAAGGAAATCACCTGATCAAAAAAGACCCCGTAGAAGCTTATCTACCAGCAAAACTGCAAAAAGCCAGTGTCATTCGATTACTTCGTATTTTAACAGTGCACCACCTGCTAAACTTGCATGTTCAACTTGTCATAAAATGGTGCCCAGGTATGACCTCATTCGGCACCTTGATGAGTCTTGTGCTAACAACGGTGTTGGTGATGACGTTCAAGTAGAGCCTGCTCAGGCTGGATTAATGAGTCCAACTGTGCCTACATCAGATTTACCCAGTGGTCCTTTAGAGAATGTGACACCTCAGAAATTATCACCACCAAAGAGAAGTTTAATTTCTGTCCAATGTGGTTCAAAACTGGGCATACAACAGCAGACCAGTCCCTACTTTAAAGATGCCTTAGTGTCCAAGGATCAAAATGAGCTTCCAAATCAGAGTGTAGAAATTATGCCTTTGGGAAGTCTGACATCGAAATTGTCCAGACGATACTTAAACGCTAAAAAATCACTCGCTAAGAATGAGGGACTAGCCAGTCAGTGCCCACAGACTTCTCCGTCCACACCTGGCACCAGCCTGACTGATAACTGTCCAGAGATGGAAGACAAAGATGAGGTTTTGAACAGTTCCCAAAAGGAAAACATTTATTCATGTGCACCTCTAAAAGAAGAGAATGCTTCAGAACAGAAGGTAAAGAACAATAAAATAACGGGAGATGAAAGCCAGAAAGCTTCCTGCGGGGAGCCAGCCCTCACCCCTGCATCTGCAGAGCATGCTTCCATATTACTGTCCTCAGATTCAACTCTCGTCAGTAACACGAAATCCTCTCCGGGAGACACTCTTGTAAAGCAAGAAAGTGCCAGAAGGGCAGATGTAGGGCTTGCTGAGCCGCTCGAGGTGCGCAGTCACAAAGAAGTACAAATGACTTTCGATGCTGCGGCCAAAACGCTGGTGTCTGGGGAGGCAGAATCAAATGGTCCTACCGATGTTGACATGTCTGACATGACTACGTGGAGTAACAACCAAGAGCTTGTCAGGGAAGCTGGCAGTGTCTTACACTGCCCTCTGGAGCAGGGGTCCAGCTGCGGTGGCCCCAGTGAGACAGCTCAACTGGCACTGAGTCACCCGTACTACCTGCGGAGCTTCCTGGTGGTGCTGCAGGCCCTCCTTGGGAACGAAGAGGACATGAAGCTCTTCGATGAGCAGGAGAAGGCGATTATCACCAGGTTTTACCAGTTGTCAGCTAGTGGTCAAAAGTTGTATGTGAGGCTCTTTCAACGTAAATTAACCTGGATTAAAATGAGTAAACTGGAGTATGAAGAGATTGCCTCCGACTTAACCCCTGTGGTTGAAGAATTGAAGGACTCAGGCTTTCTACAGACAGAATCTGAGTTGCAAGAACTCTCTGATGTACTTGAACTCCTTTCTGCTCCTGAGCTGAAAGCCCTGGCCAAAACCTTCCACTTGGTGAGTCCCGGTGGGCAGAAGCAGCAGCTGGTAGACGCCTTTCACAAACTGGCCAAACAGCGCTCAGTCTGCACGTGGGGCAAGACTCAGCCTGGAATCCGAGCAGTGATTTTAAAAAGAGCCAAAGACTTGGCTGGCCGGTCGCTTCGAGTCTGTAAAGGCCCTAGGGCTGTGTTTGCCCGAATCTTGCTTCTGTTCTCATTGACTGATTCCATGGAAGACGAAGAAGCTGCTTGTGGGGGTCAAGGTCAGCTGTCTACTGTGCTATTGGTCAATCTGGGCCGAATGGAGTTTCCTCAGTACACCATCTGCCGGAAGACCCAGATCTTCAGGGACAGAGAGGACCTCATCAGATACGCAGCAGCCGCACACATGCTGAGTGACATCTCAGCTGCCATGGCCAGTGGGAACTGGGAGGACGCTAAGGAGCTTGCTCGGAGTGCAAAAAGGGACTGGGAACAACTGAAAAGCCACCCTTCCCTGAGGTACCACGAGGCCTTGCCGCCCTTCCTGCGCTGCTTTACCGTCGGGTGGATCTACACAAGGATTTCCTCTCGGGCTGTTGAAGTCCTAGAGAGGCTTCACATGTACGAGGAAGCTGTCAAGGAACTTGAAAACCTCTTGTCTCAGAAAATCTATTGTCCTGACAGCAGAGGCCGCTGGTGGGATCGCCTGGCTCTTAACCTGCACCAGCACCTGAAGCGTCTGGAAGAGGCAATTAGGTGCATCAGGGAAGGCCTGGCAGACCCACACGTGAGGACAGGACACCGACTTTCTCTTTATCAGCGAGCCGTGCGCCTGCGAGAGTCTCCGAGCTGCAGGAAGTACAAGCACCTCTTCAGCCGGCTGCCCGAGGTTGCCGTGGGAGACGTCAAGCATGTGACCATCACGGGCAGGCTGTGCCCACAGCATGGCATGGGCAAGTCTGTGTTTGTCATGGAGAGTGGAGACGGCGCCAACCCCACCACAGTCCTGTGTTCTGTGGAGGAGCTGGCCCTGGGTTATTACAGGCAGAGTGGCTTTGACCAAGGGATTCATGGAGAAGGGTCCACCTTCAGCACCCTGTGTGGCCTCCTGCTGTGGGATATCATCTTCATGGATGGAATACCAGACGTCTTCAGAAATGCCTACCAGGCATCCCCACTGGATTTGCTCACGGACAGCTTCTTCGCAAGCAGGGAGCAGGCCCTGGAAGCCAGGCTGCAGCTGATCCACAGCGCCCCTGCTGAGAGCCTGCGGGCCTGGGTGGGCGAAGCATGGCAGGCCCAGCAAGGCAGAGTGGCCTCTCTGGTCAGCTGGGACCGCTTCACCTCCCTACAGCAAGCTCAGGATCTTGTCTCCTGCCTCGGGGGTCCTGTCCTCAGTGGTGTGTGCAGGCGCCTGGCTGCTGACTTTCGGCACTGCCGAGGGGGCCTCCCAGACTTGGTGGTGTGGAATTCTCAGAGCCACCATTGCGCCGTCAGTAGAGAACACCATTTGTAAGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMPSQRKSPDQKRPRRSLSTSKTAKSQCHSITSYFNSAPPAKLACSTCHKMVPRYD
LIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGS
LTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGDESQKASCGEPALTPAS
AEHASILLSSDSTLVSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCP
LEQGSSCGGPSETAQLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKD
SGFLQTESELQELSDVLELLSAPELKALAKTFHLVSPGGQKQQLVDAFHKLAKQRSVCTWGKTQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLF
SLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEA
LPPFLRCFTVGWIYTRISSRAVEVLERLHMYEEAVKELENLLSQKIYCPDSRGRWWDRLALNLHQHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVR
LRESPSCRKYKHLFSRLPEVAVGDVKHVTITGRLCPQHGMGKSVFVMESGDGANPTTVLCSVEELALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFM
DGIPDVFRNAYQASPLDLLTDSFFASREQALEARLQLIHSAPAESLRAWVGEAWQAQQGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFR
HCRGGLPDLVVWNSQSHHCAVSREHHL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50575
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.