cDNA Clone KIAA1390

• Plasmid: pGEX6P1 KIAA1390 E263R 110-384
• Parent Plasmid: pGEX6P1
• Expressed: GST-KIAA1390 E263R 110-384
• Tag: GST

Sequence of Insert

CTCGAGATGGAGCCAGATTTCTACTGTGTCAAGTGGATCCCTTGGAAAGGAGAACAGACACCCATCATCACCCAGAGCACTAACGGCCCTTGCCCTCTCCTTGCCATCATGAACATCCTCTTTCTTCAGTGGAAGGTGAAGCTCCCCCCGCAGAAGGAAGTGATCACATCGGATGAGCTCATGGCCCATCTTGGAAACTGCCTCCTGTCCATCAAGCCCCAGGAGAAGTCAGAGGGACTTCAGCTTAATTTTCAGCAGAATGTGGATGATGCAATGACAGTGCTGCCTAAACTGGCCACAGGTCTGGATGTCAATGTGCGATTCACAGGCGTCTCTGATTTTGAGTATACACCCGAGTGCAGTGTCTTTGACCTGCTAGGCATACCTCTGTACCATGGCTGGCTTGTTGATCCACAGAGTCCTGAGGCTGTGCGTGCAGTTGGGAAACTGAGTTACAACCAGCTGGTGCGGAGGATCATCACCTGCAAACACTCCAGTGACACCAACCTCGTGACAGAAGGCCTGATTGCAGAGCAGTTCCTGGAGACCACCGCGGCCCAGCTGACCTACCACGGACTGTGTGAGCTGACAGCAGCTGCTAAGGAGGGTGAACTTAGCGTCTTTTTCCGAAACAACCACTTTAGCACCATGACTAAGCATAAGAGTCACTTATACCTACTGGTCACTGACCAGGGCTTTCTACAGGAGGAGCAAGTCGTATGGGAGAGCCTGCACAATGTGGATGGAGACAGCTGCTTTTGTGACTCTGACTTTCACCTGAGTCATTCCCTGGGCAAGGGGCCTGGAGCAGAAGGTGGGAGTGGCTCCCCAGAATAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFPGRLEMEPDFYCVKWIPWKGEQTPIITQSTNGPCPLLAIMNILFLQWKVKLPPQKEVITSDELMAHLGNCLLSIKPQEKSEGLQLNFQQNVDDAMTVLPKLATGLDVNVRFTGVSDFEYTPECSVFDLLGIPLYHGWLVDPQSPEAVRAVGKLSYNQLVRRIITCKHSSDTNLVTEGLIAEQFLETTAAQLTYHGLCELTAAAKEGELSVFFRNNHFSTMTKHKSHLYLLVTDQGFLQEEQVVWESLHNVDGDSCFCDSDFHLSHSLGKGPGAEGGSGSPE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU47683
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please insert point mutation at position 263 in the backbone of KIA1390 (DU 47257) from Glutamate to Arginine