cDNA Clone NEDD4

• Plasmid: pGEX6P-1-Nedd4 Y43E Y585E
• Parent Plasmid: pGEX6P1
• Expressed: GST NEDD4 Y43E Y585E
• Genbank: NM_006154.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCAACTTGCGCGGTGGAGGTGTTCGGGCTCCTGGAGGACGA
GGAAAATTCACGAATTGTGAGAGTAAGAGTTATAGCCGGAATAGGCCTTG
CCAAGAAGGATATATTGGGAGCTAGTGATCCTgAaGTGAGAGTGACGTTA
TATGACCCAATGAATGGAGTTCTTACAAGTGTGCAAACAAAAACCATTAA
AAAGAGTTTGAATCCAAAGTGGAATGAAGAAATATTATTCAGAGTTCATC
CTCAGCAGCACCGGCTTCTTTTTGAAGTGTTTGACGAAAACCGATTGACA
AGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGAC
AGAAAATCCAAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTC
ATCCAAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGACTAAAAATG
ACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAACAGGC
TGAGGAATTAGAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTT
GCCATTTGCAGCAACAACAAGAACCTTCTCCTCTACCTCCAGGGTGGGAA
GAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAATCTAG
AAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTG
AGAATGGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGG
CAGATATCCGAGGAAACAGAAAGTGTTGACAACCGAGAGTCTTCCGAGAA
CTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAACCAGGCCT
TCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCA
GAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTGAGCCA
GCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCT
ATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCT
GGATTACCACCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATA
TTATGTAGATCACAATTCCAGAACGACTACTTGGACAAAGCCCACTGTAC
AGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGCAGGC
CCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCC
ATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATG
CACCAAATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACC
TGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATC
ACTTGATACTTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGA
GAACTCACACAGATGGAAGAATCTTCTACATAAATCACAATATAAAAAGA
ACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGACCAGC
AGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAA
AGTTGAAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGC
CGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATTATGGGTGTCAAGAG
AGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGG
GATTGGATgaaGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAG
GAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACGGACAA
TTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACC
TCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCAT
GGCAAACTGTTGGATGGTTTTTTCATCCGCCCATTTTACAAGATGATGCT
TCACAAACCAATAACCCTTCATGATATGGAATCTGTGGATAGTGAATATT
ACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTC
AGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCT
GAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAAT
ATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAA
ATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCAT
CAAAATTTTTGATGAAAATGAACTAGAGCTTCTTATGTGTGGACTGGGAG
ATGTTGATGTGAATGACTGGAGGGAACATACAAAGTATAAAAATGGCTAC
AGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGAT
GGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTC
GGGTGCCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAG
TCATTTACAGTTGAACAGTGGGGTACGCCTGAAAAGCTGCCAAGAGCTCA
TACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAAT
TATGGGATAAACTTCAGATGGCAATTGAAAACACCCAGGGCTTTGATGGA
GTTGATTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMATCAVEVFGLLEDEENSR
IVRVRVIAGIGLAKKDILGASDPEVRVTLYDPMNGVLTSVQTKTIKKSLN
PKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPR
LERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELE
PGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQW
KRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEII
REDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASS
SNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDH
NSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIE
QGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTS
NDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYS
RDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFL
KARLWIEFDGEKGLDEGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQ
INPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPI
TLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGG
SEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFD
ENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEK
RIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFN
RLDLPPYESFEELWDKLQMAIENTQGFDGVD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28003
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: NCBI updated (NM_006154.3) version. Clone has silent mutation at Thr857 (ACT>ACG); ; Estimated MW of expressed protein : 130983 Daltons