cDNA Clone NEDP1

• Synonyms: Den1
• Plasmid: pET28a 6His-Halo.TEV-NEDP1 C163A
• Parent Plasmid: pET28a 6His-Halo.TEV
• Expressed: 6His-HALO-NEDP1 C163A
• Genbank: NM_145204.3
• Tag: HALO
• Tag: 8Gly
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCGGTGGAGGTGGAGGTGGAGGTGGAATGGACCCCGTAGTCTTGAGTTACATGGACAGTCTACTGCGGCAATCAGATGTCTCACTATTGGATCCGCCAAGCTGGCTCAATGACCATATTATTGGGTTTGCGTTTGAGTACTTTGCCAACAGTCAGTTTCATGACTGCTCTGATCACGTCAGTTTCATCAGCCCTGAAGTCACCCAGTTCATCAAGTGCACTAGCAACCCAGCAGAGATTGCCATGTTCCTTGAACCACTGGACCTCCCCAACAAGAGAGTTGTATTTTTAGCCATCAATGATAACTCCAACCAGGCAGCTGGAGGAACCCACTGGAGTTTATTGGTCTACCTCCAAGATAAAAATAGCTTTTTTCATTATGATTCCCATAGCAGGAGCAACTCAGTTCACGCAAAGCAGGTAGCAGAGAAACTGGAGGCTTTCTTAGGCAGAAAAGGAGACAAACTGGCCTTTGTGGAAGAGAAAGCCCCTGCCCAACAAAACAGCTATGACGCTGGGATGTACGTGATATGTAACACTGAGGCCTTGTGTCAGAACTTCTTTAGGCAACAGACAGAATCACTGCTGCAGCTACTCACCCCTGCATACATCACAAAGAAGAGGGGAGAATGGAAAGATCTCATTACCACACTTGCTAAAAAGTGAGCGGCCGC

Amino Acid Sequence

MHHHHHHMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPT
SSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIE
ALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPE
FARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPF
LNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTP
GVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTL
EISGENLYFQGGSEFGGGGGGGGMDPVVLSYMDSLLRQSDVSLLDPPSWL
NDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQFIKCTSNPAEIAMFLEP
LDLPNKRVVFLAINDNSNQAAGGTHWSLLVYLQDKNSFFHYDSHSRSNSV
HAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDAGMYVICNTEALCQN
FFRQQTESLLQLLTPAYITKKRGEWKDLITTLAKK*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU28007
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 45630. N-terminal 8-Gly linker immediately upstream of ORF