cDNA Clone NEDP1

• Synonyms: Den1
• Plasmid: pET28a 6His-Halo.TEV-NEDP1 D29W A98K G99K C163A
• Parent Plasmid: pET28a 6His-Halo.TEV
• Expressed: 6His-HALO-NEDP1 D29W A98K G99K C163A
• Genbank: NM_145204.3
• Tag: HALO
• Tag: 8Gly
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCGGTGGAGGTGGAGGTGGAGGTGGAATGGACCCCGTAGTCTTGAGTTACATGGACAGTCTACTGCGGCAATCAGATGTCTCACTATTGGATCCGCCAAGCTGGCTCAATTGGCATATTATTGGGTTTGCGTTTGAGTACTTTGCCAACAGTCAGTTTCATGACTGCTCTGATCACGTCAGTTTCATCAGCCCTGAAGTCACCCAGTTCATCAAGTGCACTAGCAACCCAGCAGAGATTGCCATGTTCCTTGAACCACTGGACCTCCCCAACAAGAGAGTTGTATTTTTAGCCATCAATGATAACTCCAACCAGGCAAAGAAAGGAACCCACTGGAGTTTATTGGTCTACCTCCAAGATAAAAATAGCTTTTTTCATTATGATTCCCATAGCAGGAGCAACTCAGTTCACGCAAAGCAGGTAGCAGAGAAACTGGAGGCTTTCTTAGGCAGAAAAGGAGACAAACTGGCCTTTGTGGAAGAGAAAGCCCCTGCCCAACAAAACAGCTATGACGCTGGGATGTACGTGATATGTAACACTGAGGCCTTGTGTCAGAACTTCTTTAGGCAACAGACAGAATCACTGCTGCAGCTACTCACCCCTGCATACATCACAAAGAAGAGGGGAGAATGGAAAGATCTCATTACCACACTTGCTAAAAAGTGAGCGGCCGC

Amino Acid Sequence

MHHHHHHMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPT
SSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIE
ALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPE
FARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPF
LNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTP
GVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTL
EISGENLYFQGGSEFGGGGGGGGMDPVVLSYMDSLLRQSDVSLLDPPSWL
NWHIIGFAFEYFANSQFHDCSDHVSFISPEVTQFIKCTSNPAEIAMFLEP
LDLPNKRVVFLAINDNSNQAKKGTHWSLLVYLQDKNSFFHYDSHSRSNSV
HAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDAGMYVICNTEALCQN
FFRQQTESLLQLLTPAYITKKRGEWKDLITTLAKK*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU28008
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 45630. Clone has N-terminal 8-Gly linker immediately upstream of ORF