cDNA Clone BAZ1B

• Synonyms: WBSCR10, WBSCR9, WSTF
• Plasmid: pCMV5D HA BAZ1B
• Parent Plasmid: pCMV5D HA
• Expressed: HA BAZ1B
• Genbank: NM_032408.3
• Tag: HA
• Species: Human

Sequence of Insert

CTCGAGATGGCGCCGCTCCTGGGCCGCAAGCCCTTCCCGCTGGTGAAGCCGTTGCCCGGAGAGGAGCCGCTCTTCACCATCCCGCACACTCAGGAGGCCTTCCGCACCCGGGAAGAGTATGAAGCCCGCTTGGAAAGGTACAGTGAGCGCATTTGGACGTGCAAGAGTACTGGAAGCAGTCAGCTAACACACAAGGAAGCCTGGGAGGAAGAACAGGAAGTTGCTGAGCTTTTGAAGGAGGAGTTTCCTGCCTGGTATGAGAAGCTTGTTCTGGAAATGGTTCACCATAACACAGCCTCCTTAGAGAAGTTAGTAGATACTGCTTGGTTGGAGATCATGACCAAATATGCTGTGGGAGAAGAGTGTGACTTCGAGGTTGGGAAGGAGAAAATGCTCAAGGTGAAGATTGTGAAGATTCATCCTTTGGAGAAAGTGGATGAAGAGGCCACTGAGAAGAAATCTGATGGTGCCTGTGATTCTCCATCAAGTGACAAAGAGAACTCCAGTCAGATTGCTCAGGACCATCAGAAGAAGGAGACAGTTGTGAAAGAGGATGAAGGAAGGAGAGAGAGTATTAATGACAGAGCACGTAGATCGCCACGAAAACTTCCTACTTCATTAAAAAAAGGAGAAAGGAAATGGGCTCCTCCAAAATTTCTGCCTCACAAATATGATGTGAAACTACAAAATGAAGATAAGATCATCAGTAACGTGCCAGCAGACAGCTTGATTCGTACAGAGCGCCCACCAAATAAGGAGATAGTTCGATACTTTATACGGCATAATGCATTACGAGCTGGTACTGGTGAAAATGCACCTTGGGTCGTAGAAGATGAATTGGTGAAGAAATACTCTCTGCCCAGCAAGTTCAGTGACTTTTTACTTGATCCATACAAGTATATGACTCTCAACCCTTCTACTAAGAGGAAGAATACTGGATCCCCAGACAGGAAGCCCTCAAAGAAATCCAAGACAGACAACTCTTCTCTTAGTTCACCACTAAATCCTAAGTTATGGTGTCACGTACACTTGAAGAAGTCATTGAGTGGCTCGCCACTCAAAGTGAAGAACTCAAAGAATTCCAAATCTCCTGAAGAACATCTAGAAGAAATGATGAAGATGATGTCGCCCAATAAGCTGCACACTAACTTTCACATTCCTAAAAAAGGCCCACCTGCCAAGAAACCAGGGAAGCACAGTGACAAGCCTTTGAAGGCAAAGGGCAGAAGCAAAGGCATCCTGAATGGACAGAAATCCACAGGGAATTCCAAATCTCCCAAAAAAGGACTGAAGACTCCTAAAACCAAAATGAAGCAGATGACTTTGTTGGATATGGCCAAAGGCACGCAGAAGATGACACGAGCCCCACGGAATTCTGGGGGTACACCTAGGACCTCTAGTAAACCTCATAAACATCTGCCTCCTGCAGCCCTACACCTCATTGCATACTACAAAGAAAACAAAGACAGGGAGGACAAGAGGAGCGCCCTGTCCTGTGTTATCTCCAAAACAGCTCGTCTTCTCTCTAGTGAAGATAGAGCTCGTCTCCCAGAAGAATTGCGAAGTCTTGTTCAAAAACGCTATGAACTTCTAGAGCACAAAAAGAGGTGGGCTTCTATGTCTGAAGAACAACGGAAAGAATATTTGAAAAAGAAACGGGAGGAGCTGAAAAAGAAGTTGAAGGAAAAAGCCAAAGAACGAAGAGAGAAAGAAATGCTTGAGAGATTAGAAAAACAGAAGCGGTATGAGGACCAAGAGTTAACTGGCAAAAACCTTCCAGCATTCAGATTGGTGGATACCCCTGAAGGGCTGCCCAACACGCTGTTTGGGGATGTGGCCATGGTGGTGGAATTCTTGAGCTGTTATTCTGGGCTACTTTTACCAGATGCTCAGTATCCTATTACTGCTGTGTCCCTTATGGAAGCCTTGAGTGCAGATAAGGGTGGCTTTTTATACCTTAACAGGGTGTTGGTCATCCTCTTACAGACCCTCCTACAAGATGAGATAGCAGAAGACTATGGTGAATTGGGAATGAAGCTGTCAGAAATCCCCTTGACTCTGCATTCTGTTTCAGAGCTGGTGCGGCTCTGCTTGCGCAGATCTGATGTTCAGGAGGAAAGCGAGGGCTCAGACACAGATGACAATAAAGATTCAGCTGCATTTGAGGATAATGAGGTACAAGATGAGTTCCTAGAAAAGCTGGAGACCTCTGAATTTTTTGAGCTGACGTCAGAGGAGAAGCTACAGATCTTGACAGCACTGTGCCACCGGATCCTCATGACATACTCAGTGCAAGACCACATGGAGACCAGACAGCAGATGTCTGCAGAGTTGTGGAAGGAACGGCTTGCTGTGTTGAAGGAAGAAAATGATAAGAAGAGAGCAGAGAAACAGAAACGGAAAGAAATGGAAGCCAAAAATAAAGAAAATGGAAAAGTTGAGAATGGGTTAGGCAAAACTGATAGGAAAAAAGAAATTGTGAAGTTTGAGCCCCAAGTAGATACAGAAGCTGAAGACATGATTAGTGCTGTGAAGAGCAGAAGGTTGCTTGCCATTCAAGCTAAGAAGGAACGGGAAATCCAGGAAAGAGAAATGAAAGTGAAACTGGAACGCCAAGCTGAAGAAGAACGAATACGGAAACACAAAGCAGCTGCTGAGAAAGCTTTCCAGGAAGGGATTGCCAAGGCCAAACTAGTCATGCGCAGGACTCCTATTGGCACAGATCGAAACCATAATAGATACTGGCTCTTCTCAGATGAAGTTCCAGGATTATTCATTGAAAAAGGCTGGGTACATGACAGCATTGACTACCGATTCAACCATCACTGCAAAGACCACACAGTCTCTGGTGATGAGGATTACTGTCCTCGCAGTAAGAAAGCAAACTTAGGTAAAAATGCAAGCATGAACACACAACATGGAACAGCAACAGAAGTTGCTGTAGAGACAACCACACCCAAACAAGGACAGAACCTATGGTTTTTATGTGATAGTCAAAAGGAGCTGGATGAGTTGCTAAACTGTCTTCACCCTCAGGGAATAAGAGAAAGTCAACTTAAAGAGAGACTAGAGAAGAGGTACCAGGACATTATTCACTCTATTCATCTAGCACGGAAGCCAAATTTGGGTCTAAAATCTTGTGATGGCAACCAGGAGCTTTTAAACTTCCTTCGTAGTGATCTCATTGAAGTTGCAACAAGGTTACAAAAAGGAGGACTTGGATATGTGGAAGAAACATCAGAATTTGAAGCCCGGGTCATTTCATTAGAGAAATTGAAGGATTTTGGTGAGTGTGTGATTGCCCTTCAGGCCAGTGTCATAAAGAAATTTCTCCAAGGCTTCATGGCTCCCAAGCAAAAGAGAAGAAAACTCCAAAGTGAAGATTCAGCAAAAACTGAGGAAGTGGATGAAGAGAAGAAAATGGTAGAGGAAGCAAAGGTTGCATCTGCACTGGAGAAATGGAAGACAGCAATCCGGGAAGCTCAGACTTTCTCCAGGATGCACGTGCTGCTTGGGATGCTTGATGCCTGTATCAAGTGGGATATGTCCGCAGAAAATGCTAGGTGCAAAGTTTGTCGAAAGAAAGGTGAGGATGACAAATTGATCTTGTGTGATGAGTGTAATAAAGCCTTCCACCTGTTTTGTCTGAGGCCGGCCCTCTATGAAGTACCAGATGGTGAGTGGCAGTGCCCAGCTTGCCAGCCCGCTACTGCCAGGCGCAACTCCCGTGGCAGGAACTATACTGAAGAGTCTGCTTCTGAGGACAGTGAAGATGATGAGAGTGATGAAGAGGAGGAGGAGGAAGAAGAGGAGGAGGAGGAAGAAGATTATGAGGTGGCTGGTTTGCGATTGAGACCTCGAAAGACCATCCGGGGCAAGCACAGCGTCATCCCCCCTGCAGCAAGGTCAGGCCGGCGCCCGGGTAAGAAGCCACACTCTACCAGGAGGTCTCAGCCCAAGGCACCACCTGTGGATGATGCTGAGGTGGATGAGCTGGTGCTTCAGACCAAGCGGAGCTCCCGGAGGCAAAGCCTGGAGCTGCAGAAGTGTGAAGAGATCCTCCACAAGATCGTGAAGTACCGCTTCAGCTGGCCCTTCAGGGAGCCTGTGACCAGAGATGAGGCCGAGGACTACTATGATGTGATCACGCACCCCATGGACTTTCAGACAGTGCAGAACAAATGTTCCTGTGGGAGCTACCGCTCTGTGCAGGAGTTTCTTACTGACATGAAGCAAGTGTTTACCAATGCTGAGGTTTACAACTGCCGTGGCAGCCATGTGCTAAGCTGCATGGTGAAGACAGAACAGTGTCTAGTGGCTCTGTTGCATAAACACCTTCCTGGCCACCCATATGTCCGCAGGAAGCGCAAGAAGTTTCCTGATAGGCTTGCTGAAGATGAAGGGGACAGTGAGCCAGAGGCCGTTGGACAGTCCAGGGGACGAAGACAGAAGAAGTAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSFEEFASLEMAPLLGRKPFPLVKPLPGEEPLFTIPHTQEAFRTREEYEARLERYSERIWTCKSTGSSQLTHKEAWEEEQEVAELLKEEF
PAWYEKLVLEMVHHNTASLEKLVDTAWLEIMTKYAVGEECDFEVGKEKMLKVKIVKIHPLEKVDEEATEKKSDGACDSPSSDKENSSQIAQDHQKKETVV
KEDEGRRESINDRARRSPRKLPTSLKKGERKWAPPKFLPHKYDVKLQNEDKIISNVPADSLIRTERPPNKEIVRYFIRHNALRAGTGENAPWVVEDELVK
KYSLPSKFSDFLLDPYKYMTLNPSTKRKNTGSPDRKPSKKSKTDNSSLSSPLNPKLWCHVHLKKSLSGSPLKVKNSKNSKSPEEHLEEMMKMMSPNKLHT
NFHIPKKGPPAKKPGKHSDKPLKAKGRSKGILNGQKSTGNSKSPKKGLKTPKTKMKQMTLLDMAKGTQKMTRAPRNSGGTPRTSSKPHKHLPPAALHLIA
YYKENKDREDKRSALSCVISKTARLLSSEDRARLPEELRSLVQKRYELLEHKKRWASMSEEQRKEYLKKKREELKKKLKEKAKERREKEMLERLEKQKRY
EDQELTGKNLPAFRLVDTPEGLPNTLFGDVAMVVEFLSCYSGLLLPDAQYPITAVSLMEALSADKGGFLYLNRVLVILLQTLLQDEIAEDYGELGMKLSE
IPLTLHSVSELVRLCLRRSDVQEESEGSDTDDNKDSAAFEDNEVQDEFLEKLETSEFFELTSEEKLQILTALCHRILMTYSVQDHMETRQQMSAELWKER
LAVLKEENDKKRAEKQKRKEMEAKNKENGKVENGLGKTDRKKEIVKFEPQVDTEAEDMISAVKSRRLLAIQAKKEREIQEREMKVKLERQAEEERIRKHK
AAAEKAFQEGIAKAKLVMRRTPIGTDRNHNRYWLFSDEVPGLFIEKGWVHDSIDYRFNHHCKDHTVSGDEDYCPRSKKANLGKNASMNTQHGTATEVAVE
TTTPKQGQNLWFLCDSQKELDELLNCLHPQGIRESQLKERLEKRYQDIIHSIHLARKPNLGLKSCDGNQELLNFLRSDLIEVATRLQKGGLGYVEETSEF
EARVISLEKLKDFGECVIALQASVIKKFLQGFMAPKQKRRKLQSEDSAKTEEVDEEKKMVEEAKVASALEKWKTAIREAQTFSRMHVLLGMLDACIKWDM
SAENARCKVCRKKGEDDKLILCDECNKAFHLFCLRPALYEVPDGEWQCPACQPATARRNSRGRNYTEESASEDSEDDESDEEEEEEEEEEEEEDYEVAGL
RLRPRKTIRGKHSVIPPAARSGRRPGKKPHSTRRSQPKAPPVDDAEVDELVLQTKRSSRRQSLELQKCEEILHKIVKYRFSWPFREPVTRDEAEDYYDVI
THPMDFQTVQNKCSCGSYRSVQEFLTDMKQVFTNAEVYNCRGSHVLSCMVKTEQCLVALLHKHLPGHPYVRRKRKKFPDRLAEDEGDSEPEAVGQSRGRR
QKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5D HA BAZ1B119.17 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU52697
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains two silent nucleotide changes as compared to the reference sequence; G>A 2037 and G>A 2634 bp.; BAZ1B sequence is unstable in bacteria, grow at 30oC to prevent rearrangement.
• Low Temp: No