cDNA Clone CK1δ

• Plasmid: pCS2+ HA-CK1 delta
• Parent Plasmid: pCS2+ HA
• Expressed: HA-CK1δ
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGAGCTGAGAGTCGGGAACAGGTACCGGCTGGGCCGGAAGATCGGCAGCGGCTCCTTCGGAGACATCTATCTCGGTACGGACATTGCTGCAGGAGAAGAGGTTGCCATCAAGCTTGAATGTGTCAAAACCAAACACCCTCAGCTCCACATTGAGAGCAAAATCTACAAGATGATGCAGGGAGGAGTGGGCATCCCCACCATCAGATGGTGCGGGGCAGAGGGGGACTACAACGTCATGGTGATGGAGCTGCTGGG-GCCAAGCCTGGAGGACC-TCTTCAAC-T-TCTGCTCCAGGAAATTCAGCCTCAAAACCGTCCTGCTG-CTTGCTGACCAAATGATCAGTCGCATCGAATACATT-CATTCAAAGAACTTCATCCACCGGGATGTGAAGCCAGACAAC-TTCCTCATGGGCCTGGGGAAGAAGGGCAACCTGGTGTACATCATCGACTTC-GGGCTGGCCAAGAAGTACCGGGATGCACGCACCCACCAGCACATCCCCTATCGTGAGAACAAGAACCTCACGGGGACGGCGCGGTACGCCTCCATCAACACGCACCTTGGAATTGAACAATCCCG-AAGAGATGACTTGGAGTCTCTGGGCTACGTGCTAATGTACTTCAACCTGGGCTCTCTCCCCTGGCAGGGGCTGAAGGCTG-CCACCAAGAGACAG-AAATACGAAAGGATTAGCGAGAAGAAAATGTCCA-CCCCCATCGAAGTGTTGTGTAAAGGCTA-CCCTTCCGAATTTGCCACATACCTGAATTTCTGCCGTTCC-TTGCGTTTTGACG-ACAAGCCTGACTACT-CGTACCTGCGGCAGC-TTTTCCGGAATCT-GTTCCAT-CGCCAGGGCTTCTCCTATG-ACTACGTGTT-CGACTGGAACATG-CTCAAATTTGGTGCCAG--CCGGGCCG-CCGATG-A-CGCCGAGCGGG-AGCGC-AG-GGACC-G--AGA-GGAGCGGC-TG-AGACAC-TCGC-GG-AACCC--GGCTACCC--GC-G-G-CC-TCCC-TTCCACA-GC-CTCCGGCCGCCTG-CGGGGGACGCAGGAAGTGGCTCCCCCCACACCCCTCACCCCTACCTCACACACGGCTA-ACACCTCCCCCCGGCCCGTCTCCGGCATGGAGAGAGAGCGGAAAGTGAGTATGCGGCTGCACCGCGGGGCCCCCGTCAACATCTCCTCGTCCGACCTCACAGGCCGACAAGATACCTCTCGCATGTCCACCTCACAGAATAGCATTCCTTTCGAACACCACGGCAAGTAGCTGCTCGTCTCCCATCGGAAGGCAGCACTGGATTCCTGGTCGGGTGGCTTCCAGTGGTCTTCAGTCTGTCGTGCACCGATGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMELRVGNRYRLGRKIGSGSFGDIYLGTDIAAGEEVAIKLECVKTKHPQLHIESKIYKMMQGGVGIPTIRWCGAEGDYNVMVMELLGPS
LEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGI
EQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVF
DWNMLKFGASRAADDAERERRDREERLRHSRNPATRGLPSTASGRLRGTQEVAPPTPLTPTSHTANTSPRPVSGMERERKVSMRLHRGAPVNISSSDLTG
RQDTSRMSTSQNSIPFEHHGK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28189
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: pCS2+-HA 28162 parental. stop codon at nt 1234. Not1 site further downstream