cDNA Clone FbxO3

• Synonyms: FBA, FBX3
• Plasmid: pET15b 6HIS SUMO FBXO3
• Parent Plasmid: pET15b 6His SUMO
• Expressed: His SUMO FbxO3
• Genbank: NM_012175.3
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCATGGAGACCGAGACGGCGCCGCTGACCCTAGAGTCGCTGCCCACCGATCCCCTGCTCCTCATCTTATCCTTTTTGGACTATCGGGATCTAATCAACTGTTGTTATGTCAGTCGAAGACTTAGCCAGCTATCAAGTCATGATCCGCTGTGGAGAAGACATTGCAAAAAATACTGGCTGATATCTGAGGAAGAGAAAACACAGAAGAATCAGTGTTGGAAATCTCTCTTCATAGATACTTACTCTGATGTAGGAAGATACATTGACCATTATGCTGCTATTAAAAAGGCCTGGGATGATCTCAAGAAATATTTGGAGCCCAGGTGTCCTCGGATGGTTTTATCTCTGAAAGAGGGTGCTCGAGAGGAAGACCTCGATGCTGTGGAAGCGCAGATTGGCTGCAAGCTTCCTGACGATTATCGATGTTCATACCGAATTCACAATGGACAGAAGTTAGTGGTTCCTGGGTTATTGGGAAGCATGGCACTGTCTAATCACTATCGTTCTGAAGATTTGTTAGACGTCGATACAGCTGCCGGAGGATTCCAGCAGAGACAGGGACTGAAATACTGTCTCCCTTTAACTTTTTGCATACATACTGGTTTGAGTCAGTACATAGCAGTGGAAGCTGCAGAGGGCCGAAACAAAAATGAAGTTTTCTACCAATGTCCAGACCAAATGGCTCGAAATCCAGCTGCTATTGACATGTTTATTATAGGTGCTACTTTTACTGACTGGTTTACCTCTTATGTCAAAAATGTTGTATCAGGTGGCTTCCCCATCATCAGAGACCAAATTTTCAGATATGTTCACGATCCAGAATGTGTAGCAACAACTGGGGATATTACTGTGTCAGTTTCCACATCGTTTCTGCCAGAACTTAGCTCTGTACATCCACCCCACTATTTCTTCACATACCGAATCAGGATTGAAATGTCAAAAGATGCACTTCCTGAGAAGGCCTGTCAGTTGGACAGTCGCTATTGGAGAATAACAAATGCTAAGGGTGACGTGGAAGAAGTTCAAGGACCTGGAGTAGTTGGTGAATTTCCAATCATCAGCCCAGGTCGGGTATATGAATACACAAGCTGTACCACATTCTCTACAACATCAGGATACATGGAAGGATATTATACCTTCCATTTTCTTTACTTTAAAGACAAGATCTTTAATGTTGCCATTCCCCGATTCCATATGGCATGTCCAACATTCAGGGTGTCTATAGCCCGATTGGAAATGGGTCCTGATGAATATGAAGAGATGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAAGACGAGGATGATGATTCAGCAGATATGGATGAATCAGATGAAGATGATGAAGAGGAGAGACGGAGGAGAGTCTTTGATGTTCCCATTCGCAGACGCCGCTGCTCACGCCTTTTTTAGGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMAAMETETAPLTLESLPTDPLLLILSFLDYRDLINCCYVSRRLSQLSSHDPLWRRHCKKYWLISEEEKTQKNQCWKSLFIDTYSDVGRYIDHYAAIKKAWDDLKKYLEPRCPRMVLSLKEGAREEDLDAVEAQIGCKLPDDYRCSYRIHNGQKLVVPGLLGSMALSNHYRSEDLLDVDTAAGGFQQRQGLKYCLPLTFCIHTGLSQYIAVEAAEGRNKNEVFYQCPDQMARNPAAIDMFIIGATFTDWFTSYVKNVVSGGFPIIRDQIFRYVHDPECVATTGDITVSVSTSFLPELSSVHPPHYFFTYRIRIEMSKDALPEKACQLDSRYWRITNAKGDVEEVQGPGVVGEFPIISPGRVYEYTSCTTFSTTSGYMEGYYTFHFLYFKDKIFNVAIPRFHMACPTFRVSIARLEMGPDEYEEMEEEEEEEEEEDEDDDSADMDESDEDDEEERRRRVFDVPIRRRRCSRLF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU26313
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: linker after SENP cleavage deleted