cDNA Clone Rubicon

• Plasmid: pCMV5D GFP Rubicon
• Parent Plasmid: pCMV5D GFP
• Expressed: GFP Rubicon
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGCGGCCGGAGGGCGCGGGAATGGAGCTCGGAGGCGGCGAGGAGCGCCTGCCTGAGGAGAGCAGGAGGGAGCACTGGCAGTTGCTGGGTAATTTGAAGACGACGGTGGAGGGTTTGGTATCAACCAACAGCCCCAACGTCTGGTCTAAGTATGGTGGCTTGGAGCGGCTTTGCAGGGACATGCAGAGCATCCTCTATCACGGGCTTATCCGTGACCAGGCGTGCCGCCGCCAGACGGATTACTGGCAGTTCGTGAAAGACATCCGGTGGCTCAGTCCCCACTCAGCCCTTCACGTGGAGAAGTTCATCAGCGTGCACGAGAACGACCAGAGCAGTGCTGATGGTGCCAGTGAACGTGCTGTTGCCGAGCTGTGGCTGCAGCACAGCCTGCAGTACCACTGCCTCTCAGCCCAGCTCCGGCCCCTGCTCGGGGATAGACAGTATATCAGAAAATTCTACACAGATGCTGCCTTCCTGCTAAGTGACGCTCATGTCACGGCCATGCTGCAGTGCCTGGAAGCAGTGGAACAGAACAACCCCCGCCTCCTGGCTCAGATCGATGCGTCCATGTTTGCCAGAAAGCACGAGAGCCCGCTCCTGGTGACAAAGAGCCAGAGCCTGACAGCCCTGCCCAGTTCCACATACACCCCTCCAAACAGCTATGCTCAGCATTCCTACTTTGGGTCCTTCTCTAGCCTCCACCAATCCGTGCCCAACAATGGCTCAGAGAGAAGATCTACTTCCTTTCCACTCTCTGGCCCTCCCCGGAAACCTCAAGAAAGCAGAGGGCACGTCTCACCAGCAGAGGATCAAACCATCCAAGCCCCCCCAGTTTCAGTCTCTGCACTAGCCAGGGATTCCCCTTTGACCCCAAATGAAATGAGCTCCAGTACTCTGACCAGCCCCATAGAGGCATCCTGGGTCAGCAGCCAGAATGATTCCCCAGGTGATGCCAGTGAGGGGCCTGAGTACCTGGCCATTGGCAACTTGGACCCCCGAGGCCGGACTGCCAGCTGTCAGAGTCACAGCAGCAATGCCGAGAGCAGCAGTTCCAATTTGTTCTCCTCCAGCAGCTCCCAGAAGCCAGATTCTGCTGCCTCTTCCTTAGGGGACCAGGAAGGAGGTGGGGAGAGCCAGCTGTCCAGTGTCCTCCGCAGGTCCAGCTTCTCAGAGGGGCAGACACTCACTGTCACCAGTGGGGCAAAGAAAAGCCACATTCGCTCCCATTCGGATACCAGCATTGCCTCCAGGGGAGCTCCAGAATCCTGCAATGATAAGGCGAAGTTGAGAGGCCCTTTGCCCTACTCTGGTCAAAGCAGTGAAGTCAGCACACCCAGCTCTCTGTACATGGAATATGAAGGTGGTCGGTACCTGTGCTCAGGGGAAGGCATGTTCCGAAGACCATCAGAAGGACAGTCCCTCATCAGCTACCTCTCTGAGCAAGACTTCGGCAGCTGTGCCGACCTGGAAAAGGAGAATGCCCACTTCAGCATCTCAGAGTCCTTAATTGCTGCCATCGAGCTAATGAAGTGCAACATGATGAGCCAGTGCCTAGAGGAGGAGGAAGTGGAAGAGGAAGACAGTGATAGAGAGATCCAGGAGCTGAAGCAGAAGATCCGCCTTCGGCGCCAGCAAATCCGCACCAAGAACCTGCTCCCCATGTACCAGGAGGCTGAGCACGGAAGCTTTCGGGTCACCTCCAGCAGCTCCCAGTTCAGCTCACGTGATTCGGCACAGCTCTCTGACTCTGGCTCTGCTGATGAGGTTGATGAATTTGAAATCCAAGATGCTGACATCAGAAGGAACACAGCCTCAAGCAGCAAATCCTTCGTTTCCTCCCAGTCCTTCTCCCACTGCTTCCTGCACTCCACGTCTGCTGAGGCGGTGGCCATGGGGCTCCTGAAGCAGTTTGAGGGGATGCAGCTTCCAGCCGCCTCGGAGCTGGAGTGGCTTGTCCCGGAGCATGATGCCCCTCAGAAGCTCCTGCCCATTCCTGACTCACTGCCCATCTCACCGGATGACGGGCAGCACGCTGACATCTACAAGCTGCGGATTCGTGTTCGTGGCAACTTGGAGTGGGCCCCGCCCCGGCCTCAGATAATTTTTAATGTTCATCCAGCCCCAACGAGGAAAATTGCCGTGGCCAAGCAGAATTACCGCTGTGCAGGATGTGGCATCCGGACTGACCCTGATTACATCAAGCGACTGCGGTACTGTGAGTACCTGGGCAAGTACTTCTGCCAGTGCTGCCACGAGAATGCCCAGATGGCCATCCCCAGCCGGGTTCTGCGCAAGTGGGACTTCAGCAAGTACTACGTCAGCAACTTCTCCAAGGACCTGCTCATTAAGATCTGGAATGATCCTCTCTTCAACGTGCAGGACATAAACAGTGCCCTCTATAGGAAGGTCAAGCTGCTCAATCAAGTCCGGCTGCTGCGGGTCCAGCTGTGTCACATGAAGAACATGTTCAAGACTTGCCGACTGGCCAAGGAGCTTCTGGATTCCTTTGACACAGTCCCAGGCCACCTGACAGAGGACCTCCACCTGTACTCACTGAATGACCTGACTGCGACCAGGAAGGGGGAGCTGGGGCCCCGGCTTGCTGAGCTCACCAGGGCAGGGGCTACCCATGTGGAGAGATGCATGCTCTGCCAAGCCAAAGGCTTCATCTGTGAGTTCTGTCAGAATGAGGATGACATCATCTTTCCCTTTGAGCTCCATAAGTGCCGGACCTGTGAAGAGTGTAAAGCGTGTTACCATAAAGCCTGCTTCAAGTCTGGAAGCTGTCCGCGCTGCGAGCGGCTGCAGGCCCGGCGGGAGGCACTGGCCAGGCAGAGCCTGGAGTCTTACCTGTCAGACTACGAGGAGGAGCCCGCGGAAGCGCTGGCCCTGGAAGCCGCCGTCCTGGAGGCCACCTGA

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMRPEGAGMELGGGEERLPEESRREHWQLLGNLKTTVEGLVSTNSPNVWSKYGGLERLCRDMQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU26645
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation A96